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Role of the EGF +61A>G polymorphism in melanoma pathogenesis: an experience on a large series of Italian cases and controls.

Casula M, Alaibac M, Pizzichetta MA, Bono R, Ascierto PA, Stanganelli I, Canzanella S, Palomba G, Zattra E, Italian Melanoma Intergroup (IMI)Palmieri G - BMC Dermatol. (2009)

Bottom Line: To further evaluate this association, we conducted a case-control study in a clinic-based Italian population.Individuals with less than 10 (N = 127) or more than 100 (N = 128) benign nevi, and patients with cutaneous melanoma (N = 418) were investigated for the EGF +61A>G polymorphism, using an automated sequencing approach.Our findings further suggest that EGF +61A>G polymorphism may have a limited impact on predisposition and/or pathogenesis of melanoma and its prevalence may vary in different populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unit of Cancer Genetics, Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, Sassari, Italy. casulam@yahoo.it

ABSTRACT

Background: A single nucleotide polymorphism (61A>G) in the epidermal growth factor (EGF) gene has been implicated in both melanoma pathogenesis and increased melanoma risk. To further evaluate this association, we conducted a case-control study in a clinic-based Italian population.

Methods: Individuals with less than 10 (N = 127) or more than 100 (N = 128) benign nevi, and patients with cutaneous melanoma (N = 418) were investigated for the EGF +61A>G polymorphism, using an automated sequencing approach.

Results: Overall, no difference in EGF genotype frequencies was observed among subjects with different number of nevi as well as when non-melanoma healthy controls were compared with the melanoma patients. However, a heterogeneous distribution of the frequencies of the G/G genotype was detected among cases and controls originating from North Italy (21.1 and 18.3%, respectively) vs. those from South Italy (12.6 and 17.1%, respectively).

Conclusion: Our findings further suggest that EGF +61A>G polymorphism may have a limited impact on predisposition and/or pathogenesis of melanoma and its prevalence may vary in different populations.

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Related in: MedlinePlus

Sequencing results for the single nucleotide polymorphism at position 61 of the EGF gene. Electropherograms show the nucleotide sequences corresponding to the three different genotypes; arrows indicate the nucleotide position within the sequence.
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Figure 1: Sequencing results for the single nucleotide polymorphism at position 61 of the EGF gene. Electropherograms show the nucleotide sequences corresponding to the three different genotypes; arrows indicate the nucleotide position within the sequence.

Mentions: Figure 1 shows the nucleotide sequences for the three expected genotypes at position 61 of the EGF gene: homozygosity A/A, heterozygosity A/G, homozygosity G/G.


Role of the EGF +61A>G polymorphism in melanoma pathogenesis: an experience on a large series of Italian cases and controls.

Casula M, Alaibac M, Pizzichetta MA, Bono R, Ascierto PA, Stanganelli I, Canzanella S, Palomba G, Zattra E, Italian Melanoma Intergroup (IMI)Palmieri G - BMC Dermatol. (2009)

Sequencing results for the single nucleotide polymorphism at position 61 of the EGF gene. Electropherograms show the nucleotide sequences corresponding to the three different genotypes; arrows indicate the nucleotide position within the sequence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719594&req=5

Figure 1: Sequencing results for the single nucleotide polymorphism at position 61 of the EGF gene. Electropherograms show the nucleotide sequences corresponding to the three different genotypes; arrows indicate the nucleotide position within the sequence.
Mentions: Figure 1 shows the nucleotide sequences for the three expected genotypes at position 61 of the EGF gene: homozygosity A/A, heterozygosity A/G, homozygosity G/G.

Bottom Line: To further evaluate this association, we conducted a case-control study in a clinic-based Italian population.Individuals with less than 10 (N = 127) or more than 100 (N = 128) benign nevi, and patients with cutaneous melanoma (N = 418) were investigated for the EGF +61A>G polymorphism, using an automated sequencing approach.Our findings further suggest that EGF +61A>G polymorphism may have a limited impact on predisposition and/or pathogenesis of melanoma and its prevalence may vary in different populations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Unit of Cancer Genetics, Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, Sassari, Italy. casulam@yahoo.it

ABSTRACT

Background: A single nucleotide polymorphism (61A>G) in the epidermal growth factor (EGF) gene has been implicated in both melanoma pathogenesis and increased melanoma risk. To further evaluate this association, we conducted a case-control study in a clinic-based Italian population.

Methods: Individuals with less than 10 (N = 127) or more than 100 (N = 128) benign nevi, and patients with cutaneous melanoma (N = 418) were investigated for the EGF +61A>G polymorphism, using an automated sequencing approach.

Results: Overall, no difference in EGF genotype frequencies was observed among subjects with different number of nevi as well as when non-melanoma healthy controls were compared with the melanoma patients. However, a heterogeneous distribution of the frequencies of the G/G genotype was detected among cases and controls originating from North Italy (21.1 and 18.3%, respectively) vs. those from South Italy (12.6 and 17.1%, respectively).

Conclusion: Our findings further suggest that EGF +61A>G polymorphism may have a limited impact on predisposition and/or pathogenesis of melanoma and its prevalence may vary in different populations.

Show MeSH
Related in: MedlinePlus