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A novel bacterial isolate Stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticles.

Nangia Y, Wangoo N, Goyal N, Shekhawat G, Suri CR - Microb. Cell Fact. (2009)

Bottom Line: Gold nanoparticles were characterized and found to be of ~40 nm size.Electrophoresis, Zeta potential and FTIR measurements confirmed that the particles are capped with negatively charged phosphate groups from NADP rendering them stable in aqueous medium.The process of synthesis of well-dispersed nanoparticles using a novel microorganism isolated from the gold enriched soil sample has been reported in this study, leading to the development of an easy bioprocess for synthesis of GNPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh 160036, India. raman@imtech.res.in.

ABSTRACT

Background: The synthesis of gold nanoparticles (GNPs) has received considerable attention with their potential applications in various life sciences related applications. Recently, there has been tremendous excitement in the study of nanoparticles synthesis by using some natural biological system, which has led to the development of various biomimetic approaches for the growth of advanced nanomaterials. In the present study, we have demonstrated the synthesis of gold nanoparticles by a novel bacterial strain isolated from a site near the famous gold mines in India. A promising mechanism for the biosynthesis of GNPs by this strain and their stabilization via charge capping was investigated.

Results: A bacterial isolate capable of gold nanoparticle synthesis was isolated and identified as a novel strain of Stenotrophomonas malophilia (AuRed02) based on its morphology and an analysis of its 16S rDNA gene sequence. After 8 hrs of incubation, monodisperse preparation of gold nanoparticles was obtained. Gold nanoparticles were characterized and found to be of ~40 nm size. Electrophoresis, Zeta potential and FTIR measurements confirmed that the particles are capped with negatively charged phosphate groups from NADP rendering them stable in aqueous medium.

Conclusion: The process of synthesis of well-dispersed nanoparticles using a novel microorganism isolated from the gold enriched soil sample has been reported in this study, leading to the development of an easy bioprocess for synthesis of GNPs. This is the first study in which an extensive characterization of the indigenous bacterium isolated from the actual gold enriched soil was conducted. Promising mechanism for the biosynthesis of GNPs by the strain and their stabilization via charge capping is suggested, which involves an NADPH-dependent reductase enzyme that reduces Au3+ to Au0 through electron shuttle enzymatic metal reduction process.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree made in MEGA 3.1 software using Neighbor joining method.
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Figure 2: Phylogenetic tree made in MEGA 3.1 software using Neighbor joining method.

Mentions: The 16S rDNA sequence analysis revealed that strain AuRed02 clustered homologue being Pseudomonas Sp. (Acc. No. FJ211222). At species level, strain AuRed02 shows the highest level of sequence similarity with Stenotrophomonas maltophilia strain: BL-15 (99%) (AB194325) as shown in phylogenetic tree (Fig. 2).


A novel bacterial isolate Stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticles.

Nangia Y, Wangoo N, Goyal N, Shekhawat G, Suri CR - Microb. Cell Fact. (2009)

Phylogenetic tree made in MEGA 3.1 software using Neighbor joining method.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719591&req=5

Figure 2: Phylogenetic tree made in MEGA 3.1 software using Neighbor joining method.
Mentions: The 16S rDNA sequence analysis revealed that strain AuRed02 clustered homologue being Pseudomonas Sp. (Acc. No. FJ211222). At species level, strain AuRed02 shows the highest level of sequence similarity with Stenotrophomonas maltophilia strain: BL-15 (99%) (AB194325) as shown in phylogenetic tree (Fig. 2).

Bottom Line: Gold nanoparticles were characterized and found to be of ~40 nm size.Electrophoresis, Zeta potential and FTIR measurements confirmed that the particles are capped with negatively charged phosphate groups from NADP rendering them stable in aqueous medium.The process of synthesis of well-dispersed nanoparticles using a novel microorganism isolated from the gold enriched soil sample has been reported in this study, leading to the development of an easy bioprocess for synthesis of GNPs.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh 160036, India. raman@imtech.res.in.

ABSTRACT

Background: The synthesis of gold nanoparticles (GNPs) has received considerable attention with their potential applications in various life sciences related applications. Recently, there has been tremendous excitement in the study of nanoparticles synthesis by using some natural biological system, which has led to the development of various biomimetic approaches for the growth of advanced nanomaterials. In the present study, we have demonstrated the synthesis of gold nanoparticles by a novel bacterial strain isolated from a site near the famous gold mines in India. A promising mechanism for the biosynthesis of GNPs by this strain and their stabilization via charge capping was investigated.

Results: A bacterial isolate capable of gold nanoparticle synthesis was isolated and identified as a novel strain of Stenotrophomonas malophilia (AuRed02) based on its morphology and an analysis of its 16S rDNA gene sequence. After 8 hrs of incubation, monodisperse preparation of gold nanoparticles was obtained. Gold nanoparticles were characterized and found to be of ~40 nm size. Electrophoresis, Zeta potential and FTIR measurements confirmed that the particles are capped with negatively charged phosphate groups from NADP rendering them stable in aqueous medium.

Conclusion: The process of synthesis of well-dispersed nanoparticles using a novel microorganism isolated from the gold enriched soil sample has been reported in this study, leading to the development of an easy bioprocess for synthesis of GNPs. This is the first study in which an extensive characterization of the indigenous bacterium isolated from the actual gold enriched soil was conducted. Promising mechanism for the biosynthesis of GNPs by the strain and their stabilization via charge capping is suggested, which involves an NADPH-dependent reductase enzyme that reduces Au3+ to Au0 through electron shuttle enzymatic metal reduction process.

No MeSH data available.


Related in: MedlinePlus