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Atherosclerotic progression attenuates the expression of Nogo-B in autopsied coronary artery: pathology and virtual histology intravascular ultrasound analysis.

Lee WS, Kim SW, Hong SA, Lee TJ, Park ES, Kim HJ, Lee KJ, Kim TH, Kim CJ, Ryu WS - J. Korean Med. Sci. (2009)

Bottom Line: TkCFAs and TCFAs were defined as advanced FA.Histopathology and immunohistochemical stains demonstrated that early or late FAs had smaller necrotic cores, less empty space of decalcification, and greater Nogo-B expression compared to advanced FAs (vs. early FA, P=0.013; vs. late FA, P=0.008, respectively).Local reduction of Nogo-B may contribute to plaque formation and/or instability.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, Korea. wslee1227@dreamwiz.com

ABSTRACT
The relation of Nogo-B to atherosclerotic plaque progression is not well understood. Thus, the purpose of this study was to assess the expression of Nogo-B in fibroatheromas (FA) of different stages, classified using virtual histology intravascular ultrasound (VH-IVUS) analysis in 19 autopsied cases of non-sudden cardiac death. VH-IVUS imaging analysis was performed 30 mm from the ostium of each coronary artery. VH-IVUS revealed 11 early FAs (34.5+/-8.3 yr), 12 late FAs (42.6+/-16.6 yr), 8 thick-cap FAs (TkCFAs) (46.4+/-11.1 yr), and 6 thin-cap FAs (TCFAs) (51.8+/-6.8 yr). TkCFAs and TCFAs were defined as advanced FA. FA progression advanced with age (P=0.04). VH-IVUS analysis of small, early FAs showed smaller necrotic cores and relatively less calcium compared to more advanced FAs with large necrotic cores (P<0.001). Histopathology and immunohistochemical stains demonstrated that early or late FAs had smaller necrotic cores, less empty space of decalcification, and greater Nogo-B expression compared to advanced FAs (vs. early FA, P=0.013; vs. late FA, P=0.008, respectively). These findings suggest that FA progression is inversely associated with Nogo-B expression. Local reduction of Nogo-B may contribute to plaque formation and/or instability.

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Related in: MedlinePlus

Comparative VH-IVUS and microscopic findings between early fibroatheromas (FA) or late FAs and advanced FAs. Early FAs (A, B) had smaller necrotic cores compared to advanced FAs (F, G) with larger lipid pools. Late FAs (C) had few calcium components, while advanced FAs (H) had a large amount of calcium and decalcified empty spaces (arrows). The expression of CD68-positive macrophages (arrows) was strong in advanced FA (I) rather than in late FA (D). MMP-9 activity (arrows) was also prominent in advanced FA (J) as opposed to late FA (E). (B and G, H&E staining at ×40 magnification; C and H, von Kossa staining at ×40 and ×100 magnification, respectively; D and I, CD68 immunohistochemical staining at ×100 magnification; E and J, MMP-9 immunohistochemical staining at ×100 magnification).
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Figure 2: Comparative VH-IVUS and microscopic findings between early fibroatheromas (FA) or late FAs and advanced FAs. Early FAs (A, B) had smaller necrotic cores compared to advanced FAs (F, G) with larger lipid pools. Late FAs (C) had few calcium components, while advanced FAs (H) had a large amount of calcium and decalcified empty spaces (arrows). The expression of CD68-positive macrophages (arrows) was strong in advanced FA (I) rather than in late FA (D). MMP-9 activity (arrows) was also prominent in advanced FA (J) as opposed to late FA (E). (B and G, H&E staining at ×40 magnification; C and H, von Kossa staining at ×40 and ×100 magnification, respectively; D and I, CD68 immunohistochemical staining at ×100 magnification; E and J, MMP-9 immunohistochemical staining at ×100 magnification).

Mentions: H-E staining with VH-IVUS analysis showed that advanced FAs had larger necrotic cores than early FAs (P<0.001) and late FAs (P<0.001) did (Fig. 2A, B, F, G; Fig. 3A).


Atherosclerotic progression attenuates the expression of Nogo-B in autopsied coronary artery: pathology and virtual histology intravascular ultrasound analysis.

Lee WS, Kim SW, Hong SA, Lee TJ, Park ES, Kim HJ, Lee KJ, Kim TH, Kim CJ, Ryu WS - J. Korean Med. Sci. (2009)

Comparative VH-IVUS and microscopic findings between early fibroatheromas (FA) or late FAs and advanced FAs. Early FAs (A, B) had smaller necrotic cores compared to advanced FAs (F, G) with larger lipid pools. Late FAs (C) had few calcium components, while advanced FAs (H) had a large amount of calcium and decalcified empty spaces (arrows). The expression of CD68-positive macrophages (arrows) was strong in advanced FA (I) rather than in late FA (D). MMP-9 activity (arrows) was also prominent in advanced FA (J) as opposed to late FA (E). (B and G, H&E staining at ×40 magnification; C and H, von Kossa staining at ×40 and ×100 magnification, respectively; D and I, CD68 immunohistochemical staining at ×100 magnification; E and J, MMP-9 immunohistochemical staining at ×100 magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719206&req=5

Figure 2: Comparative VH-IVUS and microscopic findings between early fibroatheromas (FA) or late FAs and advanced FAs. Early FAs (A, B) had smaller necrotic cores compared to advanced FAs (F, G) with larger lipid pools. Late FAs (C) had few calcium components, while advanced FAs (H) had a large amount of calcium and decalcified empty spaces (arrows). The expression of CD68-positive macrophages (arrows) was strong in advanced FA (I) rather than in late FA (D). MMP-9 activity (arrows) was also prominent in advanced FA (J) as opposed to late FA (E). (B and G, H&E staining at ×40 magnification; C and H, von Kossa staining at ×40 and ×100 magnification, respectively; D and I, CD68 immunohistochemical staining at ×100 magnification; E and J, MMP-9 immunohistochemical staining at ×100 magnification).
Mentions: H-E staining with VH-IVUS analysis showed that advanced FAs had larger necrotic cores than early FAs (P<0.001) and late FAs (P<0.001) did (Fig. 2A, B, F, G; Fig. 3A).

Bottom Line: TkCFAs and TCFAs were defined as advanced FA.Histopathology and immunohistochemical stains demonstrated that early or late FAs had smaller necrotic cores, less empty space of decalcification, and greater Nogo-B expression compared to advanced FAs (vs. early FA, P=0.013; vs. late FA, P=0.008, respectively).Local reduction of Nogo-B may contribute to plaque formation and/or instability.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, Korea. wslee1227@dreamwiz.com

ABSTRACT
The relation of Nogo-B to atherosclerotic plaque progression is not well understood. Thus, the purpose of this study was to assess the expression of Nogo-B in fibroatheromas (FA) of different stages, classified using virtual histology intravascular ultrasound (VH-IVUS) analysis in 19 autopsied cases of non-sudden cardiac death. VH-IVUS imaging analysis was performed 30 mm from the ostium of each coronary artery. VH-IVUS revealed 11 early FAs (34.5+/-8.3 yr), 12 late FAs (42.6+/-16.6 yr), 8 thick-cap FAs (TkCFAs) (46.4+/-11.1 yr), and 6 thin-cap FAs (TCFAs) (51.8+/-6.8 yr). TkCFAs and TCFAs were defined as advanced FA. FA progression advanced with age (P=0.04). VH-IVUS analysis of small, early FAs showed smaller necrotic cores and relatively less calcium compared to more advanced FAs with large necrotic cores (P<0.001). Histopathology and immunohistochemical stains demonstrated that early or late FAs had smaller necrotic cores, less empty space of decalcification, and greater Nogo-B expression compared to advanced FAs (vs. early FA, P=0.013; vs. late FA, P=0.008, respectively). These findings suggest that FA progression is inversely associated with Nogo-B expression. Local reduction of Nogo-B may contribute to plaque formation and/or instability.

Show MeSH
Related in: MedlinePlus