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Expressions of uroplakins in the mouse urinary bladder with cyclophosphamide-induced cystitis.

Choi SH, Byun Y, Lee G - J. Korean Med. Sci. (2009)

Bottom Line: These all peaked within 12 hr post injection and they tended to decrease thereafter.In conclusion, CP reduced the expression of UPs.The reduction of the UPs mRNA and protein was time dependent, and this peaked within 12 hr after CP injection.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Dankook University College of Medicine, Cheonan, Korea.

ABSTRACT
Even though uroplakins (UPs) are believed to serve a strong protective barrier against toxic materials, cyclophosphamide (CP) causes extensive cystitis. We investigated the expression of UPs in the urothelium in CP induced mouse cystitis. A total of 27 ICR female mice received a single intraperitoneal injection of 200 mg CP/kg. Nine CP-treated mice and 6 controls were sequentially killed at 12, 24, and 72 hr post injection. Extensive cystitis and an increased vesical weight were seen. These all peaked within 12 hr post injection and they tended to decrease thereafter. The level of all the UPs mRNA, the protein expressions of UP II and III on immunoblotting study, and the expression of UP III on immunolocalization study were maximally suppressed within 12 hr; this partially recovered at 24 hr, and this completely recovered at 72 hr post CP injection. In conclusion, CP reduced the expression of UPs. The reduction of the UPs mRNA and protein was time dependent, and this peaked within 12 hr after CP injection. However, the damage was rapidly repaired within 24 hr. This study demonstrates a dynamic process, an extensive reduction and rapid recovery, for the UPs expression of the mouse urinary bladder after CP injection.

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Related in: MedlinePlus

Immunoblot analyses of uroplakin II and III. Cyclophosphamide reduced the expression of uroplakin II and III. Note the protein labelings of uroplakins were maximally decreased at 12 hr post CP injection, and tended to increase thereafter. As an internal standard, β-actin (Cell Signaling Technology, Danvers, MA, U.S.A.) was used.
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Figure 4: Immunoblot analyses of uroplakin II and III. Cyclophosphamide reduced the expression of uroplakin II and III. Note the protein labelings of uroplakins were maximally decreased at 12 hr post CP injection, and tended to increase thereafter. As an internal standard, β-actin (Cell Signaling Technology, Danvers, MA, U.S.A.) was used.

Mentions: A single dose of CP caused mucosal injury in the urothelium, and this was followed by a regenerative process. Significant increases in bladder wet weight were noted within 12 hr post injection, and this tended to decrease thereafter. When compared with normal bladder (Fig. 1A), the bladder revealed extensive cystitis that was characterized by acute inflammation with vascular congestion, edema and hemorrhage at 12 hr post injection (Fig. 1B). However, the submucosal edema was reduced at 24 hr (Fig. 1C) and the urothelium almost completely restored at 72 hr (Fig. 1D). A strong uroplakin III expression appeared along the bladder epithelium in the control bladder (Fig. 2A). At 12 hr after CP injection, there was a significantly decrease or loss of the uroplakin III expression in the intact bladder mucosa (Fig. 2B). However, this expression was weakly restored at 24 hr (Fig. 2C) and it was completely recovered at 72 hr (Fig. 2D). The messenger RNA expressions of all the uroplakins were significantly decreased after 12 hr and they completely recovered at 24 hr post CP injection. The protein expressions of uroplakin II and III were significantly decreased after 12 hr and they were restored 24 hr after CP injection (Fig. 3, 4).


Expressions of uroplakins in the mouse urinary bladder with cyclophosphamide-induced cystitis.

Choi SH, Byun Y, Lee G - J. Korean Med. Sci. (2009)

Immunoblot analyses of uroplakin II and III. Cyclophosphamide reduced the expression of uroplakin II and III. Note the protein labelings of uroplakins were maximally decreased at 12 hr post CP injection, and tended to increase thereafter. As an internal standard, β-actin (Cell Signaling Technology, Danvers, MA, U.S.A.) was used.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719198&req=5

Figure 4: Immunoblot analyses of uroplakin II and III. Cyclophosphamide reduced the expression of uroplakin II and III. Note the protein labelings of uroplakins were maximally decreased at 12 hr post CP injection, and tended to increase thereafter. As an internal standard, β-actin (Cell Signaling Technology, Danvers, MA, U.S.A.) was used.
Mentions: A single dose of CP caused mucosal injury in the urothelium, and this was followed by a regenerative process. Significant increases in bladder wet weight were noted within 12 hr post injection, and this tended to decrease thereafter. When compared with normal bladder (Fig. 1A), the bladder revealed extensive cystitis that was characterized by acute inflammation with vascular congestion, edema and hemorrhage at 12 hr post injection (Fig. 1B). However, the submucosal edema was reduced at 24 hr (Fig. 1C) and the urothelium almost completely restored at 72 hr (Fig. 1D). A strong uroplakin III expression appeared along the bladder epithelium in the control bladder (Fig. 2A). At 12 hr after CP injection, there was a significantly decrease or loss of the uroplakin III expression in the intact bladder mucosa (Fig. 2B). However, this expression was weakly restored at 24 hr (Fig. 2C) and it was completely recovered at 72 hr (Fig. 2D). The messenger RNA expressions of all the uroplakins were significantly decreased after 12 hr and they completely recovered at 24 hr post CP injection. The protein expressions of uroplakin II and III were significantly decreased after 12 hr and they were restored 24 hr after CP injection (Fig. 3, 4).

Bottom Line: These all peaked within 12 hr post injection and they tended to decrease thereafter.In conclusion, CP reduced the expression of UPs.The reduction of the UPs mRNA and protein was time dependent, and this peaked within 12 hr after CP injection.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Dankook University College of Medicine, Cheonan, Korea.

ABSTRACT
Even though uroplakins (UPs) are believed to serve a strong protective barrier against toxic materials, cyclophosphamide (CP) causes extensive cystitis. We investigated the expression of UPs in the urothelium in CP induced mouse cystitis. A total of 27 ICR female mice received a single intraperitoneal injection of 200 mg CP/kg. Nine CP-treated mice and 6 controls were sequentially killed at 12, 24, and 72 hr post injection. Extensive cystitis and an increased vesical weight were seen. These all peaked within 12 hr post injection and they tended to decrease thereafter. The level of all the UPs mRNA, the protein expressions of UP II and III on immunoblotting study, and the expression of UP III on immunolocalization study were maximally suppressed within 12 hr; this partially recovered at 24 hr, and this completely recovered at 72 hr post CP injection. In conclusion, CP reduced the expression of UPs. The reduction of the UPs mRNA and protein was time dependent, and this peaked within 12 hr after CP injection. However, the damage was rapidly repaired within 24 hr. This study demonstrates a dynamic process, an extensive reduction and rapid recovery, for the UPs expression of the mouse urinary bladder after CP injection.

Show MeSH
Related in: MedlinePlus