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Comparative study of the effects of different growth hormone doses on growth and spatial performance of hypophysectomized rats.

Kwak MJ, Park HJ, Nam MH, Kwon OS, Park SY, Lee SY, Kim MJ, Kim SJ, Paik KH, Jin DK - J. Korean Med. Sci. (2009)

Bottom Line: Cognitive function was evaluated using the Morris water maze (MWM) test.The results indicated that GH replacement therapy in HYPOX rats promoted an increase in the body weight and the width of the tibial growth plate in a dose-dependent manner.Therefore it is concluded that rhGH replacement therapy in HYPOX rats stimulates an increase in somatic growth in a dose-dependent manner and also has beneficial effects on cognitive functions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

ABSTRACT
This study was designed to examine the effects of recombinant human growth hormone replacement on somatic growth and cognitive function in hypophysectomized (HYPOX) female Sprague-Dawley rats. Rats (5 per group) were randomized by weight to 3 experimental groups: group 1, administered 200 microg/kg of GH once daily for 9 days; group 2, administered 200 microg/kg of GH twice daily; and group 3, administered saline daily. Somatic growth was evaluated by measurement of body weight daily and of the width of the proximal tibial growth plate of the HYPOX rats. Cognitive function was evaluated using the Morris water maze (MWM) test. The results indicated that GH replacement therapy in HYPOX rats promoted an increase in the body weight and the width of the tibial growth plate in a dose-dependent manner. On the third day of the MWM test, the escape latency in the GH-treated groups 1 and 2 was significantly shorter than that in the control rats (P<0.001 and P=0.032, respectively), suggesting that rhGH improved spatial memory acquisition in the MWM test. Therefore it is concluded that rhGH replacement therapy in HYPOX rats stimulates an increase in somatic growth in a dose-dependent manner and also has beneficial effects on cognitive functions.

Show MeSH
Effects of rhGH on body weight of HYPOX rats. Body weights were measured over 10 days. GH was administered for 9 days. Weight gain in each group was analyzed using the Wilcoxon signed-ranks test. A significant weight gain was evident only in groups 1 and 2. Data are represented as the median and interquartile ranges (5 rats per group).Group 1: rhGH 200 µg/kg subcutaneously once daily, P=0.042.Group 2: rhGH 200 µg/kg subcutaneously twice daily, P=0.043.Group 3: Saline subcutaneously once daily, P=1.000.
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Figure 2: Effects of rhGH on body weight of HYPOX rats. Body weights were measured over 10 days. GH was administered for 9 days. Weight gain in each group was analyzed using the Wilcoxon signed-ranks test. A significant weight gain was evident only in groups 1 and 2. Data are represented as the median and interquartile ranges (5 rats per group).Group 1: rhGH 200 µg/kg subcutaneously once daily, P=0.042.Group 2: rhGH 200 µg/kg subcutaneously twice daily, P=0.043.Group 3: Saline subcutaneously once daily, P=1.000.

Mentions: We compared the relative dose-dependent effects of GH on stimulation of somatic growth in HYPOX rats. The growth parameters measured included weight gain and bone growth (the width of the tibial growth plate). Cumulative body weight gain and the width of the tibial growth plate in the experimental groups are presented in Table 1. The daily body weights of the groups are shown in Fig. 2.


Comparative study of the effects of different growth hormone doses on growth and spatial performance of hypophysectomized rats.

Kwak MJ, Park HJ, Nam MH, Kwon OS, Park SY, Lee SY, Kim MJ, Kim SJ, Paik KH, Jin DK - J. Korean Med. Sci. (2009)

Effects of rhGH on body weight of HYPOX rats. Body weights were measured over 10 days. GH was administered for 9 days. Weight gain in each group was analyzed using the Wilcoxon signed-ranks test. A significant weight gain was evident only in groups 1 and 2. Data are represented as the median and interquartile ranges (5 rats per group).Group 1: rhGH 200 µg/kg subcutaneously once daily, P=0.042.Group 2: rhGH 200 µg/kg subcutaneously twice daily, P=0.043.Group 3: Saline subcutaneously once daily, P=1.000.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719187&req=5

Figure 2: Effects of rhGH on body weight of HYPOX rats. Body weights were measured over 10 days. GH was administered for 9 days. Weight gain in each group was analyzed using the Wilcoxon signed-ranks test. A significant weight gain was evident only in groups 1 and 2. Data are represented as the median and interquartile ranges (5 rats per group).Group 1: rhGH 200 µg/kg subcutaneously once daily, P=0.042.Group 2: rhGH 200 µg/kg subcutaneously twice daily, P=0.043.Group 3: Saline subcutaneously once daily, P=1.000.
Mentions: We compared the relative dose-dependent effects of GH on stimulation of somatic growth in HYPOX rats. The growth parameters measured included weight gain and bone growth (the width of the tibial growth plate). Cumulative body weight gain and the width of the tibial growth plate in the experimental groups are presented in Table 1. The daily body weights of the groups are shown in Fig. 2.

Bottom Line: Cognitive function was evaluated using the Morris water maze (MWM) test.The results indicated that GH replacement therapy in HYPOX rats promoted an increase in the body weight and the width of the tibial growth plate in a dose-dependent manner.Therefore it is concluded that rhGH replacement therapy in HYPOX rats stimulates an increase in somatic growth in a dose-dependent manner and also has beneficial effects on cognitive functions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.

ABSTRACT
This study was designed to examine the effects of recombinant human growth hormone replacement on somatic growth and cognitive function in hypophysectomized (HYPOX) female Sprague-Dawley rats. Rats (5 per group) were randomized by weight to 3 experimental groups: group 1, administered 200 microg/kg of GH once daily for 9 days; group 2, administered 200 microg/kg of GH twice daily; and group 3, administered saline daily. Somatic growth was evaluated by measurement of body weight daily and of the width of the proximal tibial growth plate of the HYPOX rats. Cognitive function was evaluated using the Morris water maze (MWM) test. The results indicated that GH replacement therapy in HYPOX rats promoted an increase in the body weight and the width of the tibial growth plate in a dose-dependent manner. On the third day of the MWM test, the escape latency in the GH-treated groups 1 and 2 was significantly shorter than that in the control rats (P<0.001 and P=0.032, respectively), suggesting that rhGH improved spatial memory acquisition in the MWM test. Therefore it is concluded that rhGH replacement therapy in HYPOX rats stimulates an increase in somatic growth in a dose-dependent manner and also has beneficial effects on cognitive functions.

Show MeSH