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Interleukin-13 and its receptors in idiopathic interstitial pneumonia: clinical implications for lung function.

Park SW, Ahn MH, Jang HK, Jang AS, Kim DJ, Koh ES, Park JS, Uh ST, Kim YH, Park JS, Paik SH, Shin HK, Youm W, Park CS - J. Korean Med. Sci. (2009)

Bottom Line: IL-13 levels were significantly higher in IPF patients than the others (P<0.05).IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients.IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

View Article: PubMed Central - PubMed

Affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.

ABSTRACT
Idiopathic interstitial pneumonia (IIP) is characterized by varying degrees of interstitial fibrosis. IL-13 and IL-4 are strong inducers of tissue fibrosis, whereas IFN-gamma has antifibrotic potential. However, the roles of these substances in IIP remain unknown. IL-13, IL-4, and IFN-gamma were measured in the BAL fluid of 16 idiopathic pulmonary fibrosis (IPF) patients, 10 nonspecific interstitial pneumonia (NSIP) patients, and 8 normal controls. The expression of IL-13 and IL-13Ralpha1/alpha2 in lung tissues was analyzed using ELISA and immunohistochemistry. IL-13 levels were significantly higher in IPF patients than the others (P<0.05). IL-4 levels were higher in both IPF and NSIP patients than in normal controls (P<0.05), and IFN-gamma levels were lower in NSIP patients than in normal controls (P=0.047). IL-13 levels correlated inversely with FVC% (r=-0.47, P=0.043) and DLCO% (r=-0.58, P=0.014) in IPF and NSIP patients. IL-13 was strongly expressed in the smooth muscle, bronchial epithelium, alveolar macrophages and endothelium of IPF patients. IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients. IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

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Immunohistochemical analysis of IL-13 and IL-13Rα1/IL-13Rα2 expression in lung tissues from patients with IPF and from controls. IL-13 was only negligibly expressed in controls (A) but was strongly expressed in the smooth muscle (B), bronchial epithelium, especially the hyperplastic regenerating epithelium (C), alveolar macrophages (D), endothelium (E), and interstitum (F) of IPF patients. IL-13Rα1 was strongly expressed by the bronchial epithelium and smooth muscle of IPF patients (G). IL-13Rα2 was weakly expressed in the same regions of IPF patients (H). Magnification: ×20 (A), ×40 (B), ×100 (C, F-H), and ×200 (D, E).
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Figure 3: Immunohistochemical analysis of IL-13 and IL-13Rα1/IL-13Rα2 expression in lung tissues from patients with IPF and from controls. IL-13 was only negligibly expressed in controls (A) but was strongly expressed in the smooth muscle (B), bronchial epithelium, especially the hyperplastic regenerating epithelium (C), alveolar macrophages (D), endothelium (E), and interstitum (F) of IPF patients. IL-13Rα1 was strongly expressed by the bronchial epithelium and smooth muscle of IPF patients (G). IL-13Rα2 was weakly expressed in the same regions of IPF patients (H). Magnification: ×20 (A), ×40 (B), ×100 (C, F-H), and ×200 (D, E).

Mentions: Immunohistochemical staining of IL-13, IL-13Rα1, and IL-13Rα2 was performed in the lung tissues of controls (n=4), IPF patients (n=6), and NSIP patients showing fibrosis (n=6). To avoid the smoking effect, never smokers were selected from the study subjects. Control subjects had undergone a lobectomy to remove localized solitary pulmonary nodules (two lung cancer and two tuberculoma). Both IL-13 and IL-13Rα1 were strongly expressed in the bronchial epithelial cells, alveolar macrophages, endothelial cells, and smooth muscle of all patients (Fig. 3), although semiquantitative evaluation showed that their intensities were much stronger in IPF patients than in NSIP patients (Table 2). IL-13Rα2 showed the same pattern of expression as IL-13Rα1 but weaker staining in both patient groups (Table 2, Fig. 3). Neither IL-13Rα1 nor IL-13Rα2 expression was detected in the four control lung tissues.


Interleukin-13 and its receptors in idiopathic interstitial pneumonia: clinical implications for lung function.

Park SW, Ahn MH, Jang HK, Jang AS, Kim DJ, Koh ES, Park JS, Uh ST, Kim YH, Park JS, Paik SH, Shin HK, Youm W, Park CS - J. Korean Med. Sci. (2009)

Immunohistochemical analysis of IL-13 and IL-13Rα1/IL-13Rα2 expression in lung tissues from patients with IPF and from controls. IL-13 was only negligibly expressed in controls (A) but was strongly expressed in the smooth muscle (B), bronchial epithelium, especially the hyperplastic regenerating epithelium (C), alveolar macrophages (D), endothelium (E), and interstitum (F) of IPF patients. IL-13Rα1 was strongly expressed by the bronchial epithelium and smooth muscle of IPF patients (G). IL-13Rα2 was weakly expressed in the same regions of IPF patients (H). Magnification: ×20 (A), ×40 (B), ×100 (C, F-H), and ×200 (D, E).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719183&req=5

Figure 3: Immunohistochemical analysis of IL-13 and IL-13Rα1/IL-13Rα2 expression in lung tissues from patients with IPF and from controls. IL-13 was only negligibly expressed in controls (A) but was strongly expressed in the smooth muscle (B), bronchial epithelium, especially the hyperplastic regenerating epithelium (C), alveolar macrophages (D), endothelium (E), and interstitum (F) of IPF patients. IL-13Rα1 was strongly expressed by the bronchial epithelium and smooth muscle of IPF patients (G). IL-13Rα2 was weakly expressed in the same regions of IPF patients (H). Magnification: ×20 (A), ×40 (B), ×100 (C, F-H), and ×200 (D, E).
Mentions: Immunohistochemical staining of IL-13, IL-13Rα1, and IL-13Rα2 was performed in the lung tissues of controls (n=4), IPF patients (n=6), and NSIP patients showing fibrosis (n=6). To avoid the smoking effect, never smokers were selected from the study subjects. Control subjects had undergone a lobectomy to remove localized solitary pulmonary nodules (two lung cancer and two tuberculoma). Both IL-13 and IL-13Rα1 were strongly expressed in the bronchial epithelial cells, alveolar macrophages, endothelial cells, and smooth muscle of all patients (Fig. 3), although semiquantitative evaluation showed that their intensities were much stronger in IPF patients than in NSIP patients (Table 2). IL-13Rα2 showed the same pattern of expression as IL-13Rα1 but weaker staining in both patient groups (Table 2, Fig. 3). Neither IL-13Rα1 nor IL-13Rα2 expression was detected in the four control lung tissues.

Bottom Line: IL-13 levels were significantly higher in IPF patients than the others (P<0.05).IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients.IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

View Article: PubMed Central - PubMed

Affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.

ABSTRACT
Idiopathic interstitial pneumonia (IIP) is characterized by varying degrees of interstitial fibrosis. IL-13 and IL-4 are strong inducers of tissue fibrosis, whereas IFN-gamma has antifibrotic potential. However, the roles of these substances in IIP remain unknown. IL-13, IL-4, and IFN-gamma were measured in the BAL fluid of 16 idiopathic pulmonary fibrosis (IPF) patients, 10 nonspecific interstitial pneumonia (NSIP) patients, and 8 normal controls. The expression of IL-13 and IL-13Ralpha1/alpha2 in lung tissues was analyzed using ELISA and immunohistochemistry. IL-13 levels were significantly higher in IPF patients than the others (P<0.05). IL-4 levels were higher in both IPF and NSIP patients than in normal controls (P<0.05), and IFN-gamma levels were lower in NSIP patients than in normal controls (P=0.047). IL-13 levels correlated inversely with FVC% (r=-0.47, P=0.043) and DLCO% (r=-0.58, P=0.014) in IPF and NSIP patients. IL-13 was strongly expressed in the smooth muscle, bronchial epithelium, alveolar macrophages and endothelium of IPF patients. IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients. IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

Show MeSH
Related in: MedlinePlus