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Interleukin-13 and its receptors in idiopathic interstitial pneumonia: clinical implications for lung function.

Park SW, Ahn MH, Jang HK, Jang AS, Kim DJ, Koh ES, Park JS, Uh ST, Kim YH, Park JS, Paik SH, Shin HK, Youm W, Park CS - J. Korean Med. Sci. (2009)

Bottom Line: IL-13 levels were significantly higher in IPF patients than the others (P<0.05).IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients.IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

View Article: PubMed Central - PubMed

Affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.

ABSTRACT
Idiopathic interstitial pneumonia (IIP) is characterized by varying degrees of interstitial fibrosis. IL-13 and IL-4 are strong inducers of tissue fibrosis, whereas IFN-gamma has antifibrotic potential. However, the roles of these substances in IIP remain unknown. IL-13, IL-4, and IFN-gamma were measured in the BAL fluid of 16 idiopathic pulmonary fibrosis (IPF) patients, 10 nonspecific interstitial pneumonia (NSIP) patients, and 8 normal controls. The expression of IL-13 and IL-13Ralpha1/alpha2 in lung tissues was analyzed using ELISA and immunohistochemistry. IL-13 levels were significantly higher in IPF patients than the others (P<0.05). IL-4 levels were higher in both IPF and NSIP patients than in normal controls (P<0.05), and IFN-gamma levels were lower in NSIP patients than in normal controls (P=0.047). IL-13 levels correlated inversely with FVC% (r=-0.47, P=0.043) and DLCO% (r=-0.58, P=0.014) in IPF and NSIP patients. IL-13 was strongly expressed in the smooth muscle, bronchial epithelium, alveolar macrophages and endothelium of IPF patients. IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients. IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

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IL-4, IL-13, and IFN-γ levels in the BAL fluids of IPF and NSIP patients and of normal controls. Horizontal bars represent the mean levels of the three groups.
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Figure 1: IL-4, IL-13, and IFN-γ levels in the BAL fluids of IPF and NSIP patients and of normal controls. Horizontal bars represent the mean levels of the three groups.

Mentions: IL-13 activity was detected in the BAL fluid of all patients and in seven of the eight control subjects. The IL-13 levels were higher in IPF patients than in NSIP patients (249.0±83.1 vs. 137.7±37.9 pg/mL, P=0.032) and controls (249.0±83.1 vs. 49.0±13.1 pg/mL, P=0.012). The IL-13 levels were also higher in NSIP patients than in controls (P=0.041; Fig. 1A). IL-4 activity was detected in 12 of 16 IPF patients, eight of ten NSIP patients, and three of eight controls. The mean IL-4 level was significantly higher in the IPF and NSIP groups than in the control group (16.3±4.5 and 12.3±3.35 pg/mL vs. 4.67±2.28 pg/mL, P=0.008 and P=0.034, respectively). The IL-4 level was not significantly different between the IPF and NSIP groups (P>0.05; Fig. 1B). IFN-γ activity was detected in 14 of 16 IPF patients, nine of ten NSIP patients, and seven of eight controls. Both patient groups had low INF-γ levels compared with the control group (7.72±3.02 and 3.82±1.59 pg/mL vs. 11.32±3.64 pg/mL; Fig. 1C), but only the difference between NSIP patients and controls was significant (P=0.047). The IL-13 level was inversely correlated with DLCO (% pred) (n=26, r=-0.58, P=0.014) and FVC (% pred) (n=26, r=-0.47, P=0.043) in NSIP and IPF patients (Fig. 2). Neither the IL-4 nor INF-γ level correlated with the physiological parameters (data not shown).


Interleukin-13 and its receptors in idiopathic interstitial pneumonia: clinical implications for lung function.

Park SW, Ahn MH, Jang HK, Jang AS, Kim DJ, Koh ES, Park JS, Uh ST, Kim YH, Park JS, Paik SH, Shin HK, Youm W, Park CS - J. Korean Med. Sci. (2009)

IL-4, IL-13, and IFN-γ levels in the BAL fluids of IPF and NSIP patients and of normal controls. Horizontal bars represent the mean levels of the three groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2719183&req=5

Figure 1: IL-4, IL-13, and IFN-γ levels in the BAL fluids of IPF and NSIP patients and of normal controls. Horizontal bars represent the mean levels of the three groups.
Mentions: IL-13 activity was detected in the BAL fluid of all patients and in seven of the eight control subjects. The IL-13 levels were higher in IPF patients than in NSIP patients (249.0±83.1 vs. 137.7±37.9 pg/mL, P=0.032) and controls (249.0±83.1 vs. 49.0±13.1 pg/mL, P=0.012). The IL-13 levels were also higher in NSIP patients than in controls (P=0.041; Fig. 1A). IL-4 activity was detected in 12 of 16 IPF patients, eight of ten NSIP patients, and three of eight controls. The mean IL-4 level was significantly higher in the IPF and NSIP groups than in the control group (16.3±4.5 and 12.3±3.35 pg/mL vs. 4.67±2.28 pg/mL, P=0.008 and P=0.034, respectively). The IL-4 level was not significantly different between the IPF and NSIP groups (P>0.05; Fig. 1B). IFN-γ activity was detected in 14 of 16 IPF patients, nine of ten NSIP patients, and seven of eight controls. Both patient groups had low INF-γ levels compared with the control group (7.72±3.02 and 3.82±1.59 pg/mL vs. 11.32±3.64 pg/mL; Fig. 1C), but only the difference between NSIP patients and controls was significant (P=0.047). The IL-13 level was inversely correlated with DLCO (% pred) (n=26, r=-0.58, P=0.014) and FVC (% pred) (n=26, r=-0.47, P=0.043) in NSIP and IPF patients (Fig. 2). Neither the IL-4 nor INF-γ level correlated with the physiological parameters (data not shown).

Bottom Line: IL-13 levels were significantly higher in IPF patients than the others (P<0.05).IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients.IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

View Article: PubMed Central - PubMed

Affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.

ABSTRACT
Idiopathic interstitial pneumonia (IIP) is characterized by varying degrees of interstitial fibrosis. IL-13 and IL-4 are strong inducers of tissue fibrosis, whereas IFN-gamma has antifibrotic potential. However, the roles of these substances in IIP remain unknown. IL-13, IL-4, and IFN-gamma were measured in the BAL fluid of 16 idiopathic pulmonary fibrosis (IPF) patients, 10 nonspecific interstitial pneumonia (NSIP) patients, and 8 normal controls. The expression of IL-13 and IL-13Ralpha1/alpha2 in lung tissues was analyzed using ELISA and immunohistochemistry. IL-13 levels were significantly higher in IPF patients than the others (P<0.05). IL-4 levels were higher in both IPF and NSIP patients than in normal controls (P<0.05), and IFN-gamma levels were lower in NSIP patients than in normal controls (P=0.047). IL-13 levels correlated inversely with FVC% (r=-0.47, P=0.043) and DLCO% (r=-0.58, P=0.014) in IPF and NSIP patients. IL-13 was strongly expressed in the smooth muscle, bronchial epithelium, alveolar macrophages and endothelium of IPF patients. IL-13Ralpha1, rather than IL-13Ralpha2, was strongly expressed in the smooth muscle, bronchial epithelium, and endothelium of IPF patients. IL-13 and its receptors may contribute to the pathogenesis of fibrosis in IIP and appear to be related to the severity of the disease.

Show MeSH
Related in: MedlinePlus