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p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway.

Shi Y, Song Y, Wang Y, Liang X, Hu Y, Guan X, Cheng J, Yang K - J. Biomed. Biotechnol. (2009)

Bottom Line: It has antiandrogenic effect.The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC).Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

ABSTRACT
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p'-DDE-induced toxicity in male reproductive system. The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p'-DDE treatment in these cells. The activation of NF-kappaB was enhanced with the increase of p,p'-DDE dose. Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

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Effect of p,p′-DDE on NF-κB activation in rat Sertoli cells by laser scanning confocal microscope (40×). Representative images for NF-κB activation in control (a), 10 μM (b), 30 μM (c), and 50 μM (d) p,p′-DDE-treated groups. There was a dose-dependent increase in nuclear translocation of NF-κB in Sertoli cells. In 50 μM p,p′-DDE-treated group, the nuclear translocation was more pronounced.
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fig6: Effect of p,p′-DDE on NF-κB activation in rat Sertoli cells by laser scanning confocal microscope (40×). Representative images for NF-κB activation in control (a), 10 μM (b), 30 μM (c), and 50 μM (d) p,p′-DDE-treated groups. There was a dose-dependent increase in nuclear translocation of NF-κB in Sertoli cells. In 50 μM p,p′-DDE-treated group, the nuclear translocation was more pronounced.

Mentions: Nuclear translocation of NF-κB in rat Sertoli cells was observed by laser scanning confocal microscopy. As seen in Figure 6, morphologically, p65 protein and nuclei exhibited red and blue fluorescence, respectively, and could be simultaneously viewed under laser scanning confocal microscope. The activation of NF-κB enhanced with the increase of p,p′-DDE dose.


p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway.

Shi Y, Song Y, Wang Y, Liang X, Hu Y, Guan X, Cheng J, Yang K - J. Biomed. Biotechnol. (2009)

Effect of p,p′-DDE on NF-κB activation in rat Sertoli cells by laser scanning confocal microscope (40×). Representative images for NF-κB activation in control (a), 10 μM (b), 30 μM (c), and 50 μM (d) p,p′-DDE-treated groups. There was a dose-dependent increase in nuclear translocation of NF-κB in Sertoli cells. In 50 μM p,p′-DDE-treated group, the nuclear translocation was more pronounced.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2717566&req=5

fig6: Effect of p,p′-DDE on NF-κB activation in rat Sertoli cells by laser scanning confocal microscope (40×). Representative images for NF-κB activation in control (a), 10 μM (b), 30 μM (c), and 50 μM (d) p,p′-DDE-treated groups. There was a dose-dependent increase in nuclear translocation of NF-κB in Sertoli cells. In 50 μM p,p′-DDE-treated group, the nuclear translocation was more pronounced.
Mentions: Nuclear translocation of NF-κB in rat Sertoli cells was observed by laser scanning confocal microscopy. As seen in Figure 6, morphologically, p65 protein and nuclei exhibited red and blue fluorescence, respectively, and could be simultaneously viewed under laser scanning confocal microscope. The activation of NF-κB enhanced with the increase of p,p′-DDE dose.

Bottom Line: It has antiandrogenic effect.The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC).Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

ABSTRACT
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p'-DDE-induced toxicity in male reproductive system. The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p'-DDE treatment in these cells. The activation of NF-kappaB was enhanced with the increase of p,p'-DDE dose. Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

Show MeSH
Related in: MedlinePlus