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p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway.

Shi Y, Song Y, Wang Y, Liang X, Hu Y, Guan X, Cheng J, Yang K - J. Biomed. Biotechnol. (2009)

Bottom Line: It has antiandrogenic effect.The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC).Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

ABSTRACT
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p'-DDE-induced toxicity in male reproductive system. The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p'-DDE treatment in these cells. The activation of NF-kappaB was enhanced with the increase of p,p'-DDE dose. Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

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Morphological observation of apoptotic effects of Sertoli cells treated with p,p′-DDE with or without NAC under a fluorescence microscope. Cells in control group (a) and cells treated with 10, 30, 50 μM p,p′-DDE, and 50 μM p,p′-DDE with NAC (b, c, d, and e) were observed by AO/EB staining. Original magnification is ×40.
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fig2: Morphological observation of apoptotic effects of Sertoli cells treated with p,p′-DDE with or without NAC under a fluorescence microscope. Cells in control group (a) and cells treated with 10, 30, 50 μM p,p′-DDE, and 50 μM p,p′-DDE with NAC (b, c, d, and e) were observed by AO/EB staining. Original magnification is ×40.

Mentions: AO/EB double staining: morphologically, the early apoptotic cells, because of membrane integrity, exhibited stained green with AO, some nuclei exhibited bright condensed or fragmented chromatin, and the late apoptotic cells, because of the lost membrane integrity, were stained red with EB. Sertoli cells with 30 or 50 μM p,p′-DDE treatment, due to the increase in cell membrane permeability, showed green and red fluorescence and exhibited nucleus fragment, which could be blocked by preincubation with NAC (Figure 2).


p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway.

Shi Y, Song Y, Wang Y, Liang X, Hu Y, Guan X, Cheng J, Yang K - J. Biomed. Biotechnol. (2009)

Morphological observation of apoptotic effects of Sertoli cells treated with p,p′-DDE with or without NAC under a fluorescence microscope. Cells in control group (a) and cells treated with 10, 30, 50 μM p,p′-DDE, and 50 μM p,p′-DDE with NAC (b, c, d, and e) were observed by AO/EB staining. Original magnification is ×40.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2717566&req=5

fig2: Morphological observation of apoptotic effects of Sertoli cells treated with p,p′-DDE with or without NAC under a fluorescence microscope. Cells in control group (a) and cells treated with 10, 30, 50 μM p,p′-DDE, and 50 μM p,p′-DDE with NAC (b, c, d, and e) were observed by AO/EB staining. Original magnification is ×40.
Mentions: AO/EB double staining: morphologically, the early apoptotic cells, because of membrane integrity, exhibited stained green with AO, some nuclei exhibited bright condensed or fragmented chromatin, and the late apoptotic cells, because of the lost membrane integrity, were stained red with EB. Sertoli cells with 30 or 50 μM p,p′-DDE treatment, due to the increase in cell membrane permeability, showed green and red fluorescence and exhibited nucleus fragment, which could be blocked by preincubation with NAC (Figure 2).

Bottom Line: It has antiandrogenic effect.The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC).Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

ABSTRACT
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p'-DDE-induced toxicity in male reproductive system. The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p'-DDE treatment in these cells. The activation of NF-kappaB was enhanced with the increase of p,p'-DDE dose. Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

Show MeSH
Related in: MedlinePlus