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p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway.

Shi Y, Song Y, Wang Y, Liang X, Hu Y, Guan X, Cheng J, Yang K - J. Biomed. Biotechnol. (2009)

Bottom Line: It has antiandrogenic effect.The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC).Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

ABSTRACT
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p'-DDE-induced toxicity in male reproductive system. The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p'-DDE treatment in these cells. The activation of NF-kappaB was enhanced with the increase of p,p'-DDE dose. Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

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Related in: MedlinePlus

Effect of p,p′-DDE on the viability of rat Sertoli cells with MTT assay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) can be reduced by the mitochondrial dehydrogenase of viable cells to blue formazan product which can be measured spectrophotometrically. Cells were treated with 0.3% DMSO, 10, 30, 50 or 70 μM p,p′-DDE for 24 hours, and the optical density (OD) was measured by MTT. Cellular viability (%) was calculated using the following equation: cellular viability (%)=(ODtreatment/ODcontrol) × 100. Significant difference is (*) P < .05, compared with the control group.
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fig1: Effect of p,p′-DDE on the viability of rat Sertoli cells with MTT assay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) can be reduced by the mitochondrial dehydrogenase of viable cells to blue formazan product which can be measured spectrophotometrically. Cells were treated with 0.3% DMSO, 10, 30, 50 or 70 μM p,p′-DDE for 24 hours, and the optical density (OD) was measured by MTT. Cellular viability (%) was calculated using the following equation: cellular viability (%)=(ODtreatment/ODcontrol) × 100. Significant difference is (*) P < .05, compared with the control group.

Mentions: Sertoli cells were treated with 10, 30, 50, or 70 μM p,p′-DDE for 24 hours and analyzed with MTT assay. As shown in Figure 1, the viability of Sertoli cells was reduced after treatment with 50 or 70 μM p,p′-DDE (P < .05). In 70 μM p,p′-DDE-treated group, the cellular viability was about 53.54% of that in control group. Based on this result, p,p′-DDE at concentration of 10, 30, or 50 μM was used in subsequent experiments.


p,p'-DDE induces apoptosis of rat Sertoli cells via a FasL-dependent pathway.

Shi Y, Song Y, Wang Y, Liang X, Hu Y, Guan X, Cheng J, Yang K - J. Biomed. Biotechnol. (2009)

Effect of p,p′-DDE on the viability of rat Sertoli cells with MTT assay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) can be reduced by the mitochondrial dehydrogenase of viable cells to blue formazan product which can be measured spectrophotometrically. Cells were treated with 0.3% DMSO, 10, 30, 50 or 70 μM p,p′-DDE for 24 hours, and the optical density (OD) was measured by MTT. Cellular viability (%) was calculated using the following equation: cellular viability (%)=(ODtreatment/ODcontrol) × 100. Significant difference is (*) P < .05, compared with the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2717566&req=5

fig1: Effect of p,p′-DDE on the viability of rat Sertoli cells with MTT assay. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) can be reduced by the mitochondrial dehydrogenase of viable cells to blue formazan product which can be measured spectrophotometrically. Cells were treated with 0.3% DMSO, 10, 30, 50 or 70 μM p,p′-DDE for 24 hours, and the optical density (OD) was measured by MTT. Cellular viability (%) was calculated using the following equation: cellular viability (%)=(ODtreatment/ODcontrol) × 100. Significant difference is (*) P < .05, compared with the control group.
Mentions: Sertoli cells were treated with 10, 30, 50, or 70 μM p,p′-DDE for 24 hours and analyzed with MTT assay. As shown in Figure 1, the viability of Sertoli cells was reduced after treatment with 50 or 70 μM p,p′-DDE (P < .05). In 70 μM p,p′-DDE-treated group, the cellular viability was about 53.54% of that in control group. Based on this result, p,p′-DDE at concentration of 10, 30, or 50 μM was used in subsequent experiments.

Bottom Line: It has antiandrogenic effect.The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC).Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

View Article: PubMed Central - PubMed

Affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

ABSTRACT
One,1-dichloro-2,2 bis(p-chlorophenyl) ethylene (p,p'-DDE), the major metabolite of 2,2-bis(4-Chlorophenyl)-1,1,1-trichloroethane (DDT), is a known persistent organic pollutant and male reproductive toxicant. It has antiandrogenic effect. However, the mechanism by which p,p'-DDE exposure causes male reproductive toxicity remains unknown. In the present study, rat Sertoli cells were used to investigate the molecular mechanism involved in p,p'-DDE-induced toxicity in male reproductive system. The results indicated that p,p'-DDE exposure at over 30 muM showed the induction of apoptotic cell death. p,p'-DDE could induce increases in FasL mRNA and protein, which could be blocked by an antioxidant agent, N-acetyl-l-cysteine (NAC). In addition, caspase-3 and -8 were activated by p,p'-DDE treatment in these cells. The activation of NF-kappaB was enhanced with the increase of p,p'-DDE dose. Taken together, these results suggested that exposure to p,p'-DDE might induce apoptosis of rat Sertoli cells through a FasL-dependent pathway.

Show MeSH
Related in: MedlinePlus