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Translocation detection in lymphoma diagnosis by split-signal FISH: a standardised approach.

van Rijk A, Mason D, Jones M, Cabeçadas J, Crespo M, Cigudosa JC, Garcia JF, Leoncini L, Cocco M, Hansmann ML, Mottok A, Copie Bergman C, Baia M, Anagnostou D, Pouliou E, Hamilton Dutoit S, Hjøllund Christiansen M, Svenstrup Poulsen T, Hauge Matthiesen S, van Dongen J, van Krieken JH - J Hematop (2008)

Bottom Line: The histopathological diagnosis is generally considered difficult and prone to mistakes.Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications.The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology-824, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands, a.vanrijk@pathol.umcn.nl.

ABSTRACT
Lymphomas originating from the lymphatic system comprise about 30 entities classified according to the World Health Organization (WHO). The histopathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in different lymphoma entities, their detection will be increasingly important. Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications. The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities. Therefore, 16 fluorescent probes and 10 WHO entities, supplemented with reactive cases, were selected. The results of the Euro-FISH programme show that all probes were correctly cytogenetically located, that the standardised protocol is robust, resulting in reliable results in approximately 90% of cases, and that the procedure could be implemented in every laboratory, bringing the relatively easy interpretation of split-signal probes within the reach of many pathology laboratories.

No MeSH data available.


Related in: MedlinePlus

Selection of probes validated in stage 0. a BCL10 localises to chromosomal band 1p22; b BCL6 localises to chromosomal band 3q27; c ALK localises to chromosomal band 2p23. a and b A normal DAPI (DNA) fluorescence staining combined with the FISH probe signal. c An inverted DAPI staining in combination with the FISH probe signal
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Fig2: Selection of probes validated in stage 0. a BCL10 localises to chromosomal band 1p22; b BCL6 localises to chromosomal band 3q27; c ALK localises to chromosomal band 2p23. a and b A normal DAPI (DNA) fluorescence staining combined with the FISH probe signal. c An inverted DAPI staining in combination with the FISH probe signal

Mentions: During stage 0, metaphase slides made from B lymphocytes of healthy donors were used to validate all 16 probes in duplicate. Since eight laboratories each tested four probes, duplicates were independently scored. In order to properly validate the probes, five metaphases per probe per laboratory were analysed. All probes localised to the expected position (Table 1) and no irregularities were found. A selection of the probes validated in this stage is shown in Fig. 2.Fig. 2


Translocation detection in lymphoma diagnosis by split-signal FISH: a standardised approach.

van Rijk A, Mason D, Jones M, Cabeçadas J, Crespo M, Cigudosa JC, Garcia JF, Leoncini L, Cocco M, Hansmann ML, Mottok A, Copie Bergman C, Baia M, Anagnostou D, Pouliou E, Hamilton Dutoit S, Hjøllund Christiansen M, Svenstrup Poulsen T, Hauge Matthiesen S, van Dongen J, van Krieken JH - J Hematop (2008)

Selection of probes validated in stage 0. a BCL10 localises to chromosomal band 1p22; b BCL6 localises to chromosomal band 3q27; c ALK localises to chromosomal band 2p23. a and b A normal DAPI (DNA) fluorescence staining combined with the FISH probe signal. c An inverted DAPI staining in combination with the FISH probe signal
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2713488&req=5

Fig2: Selection of probes validated in stage 0. a BCL10 localises to chromosomal band 1p22; b BCL6 localises to chromosomal band 3q27; c ALK localises to chromosomal band 2p23. a and b A normal DAPI (DNA) fluorescence staining combined with the FISH probe signal. c An inverted DAPI staining in combination with the FISH probe signal
Mentions: During stage 0, metaphase slides made from B lymphocytes of healthy donors were used to validate all 16 probes in duplicate. Since eight laboratories each tested four probes, duplicates were independently scored. In order to properly validate the probes, five metaphases per probe per laboratory were analysed. All probes localised to the expected position (Table 1) and no irregularities were found. A selection of the probes validated in this stage is shown in Fig. 2.Fig. 2

Bottom Line: The histopathological diagnosis is generally considered difficult and prone to mistakes.Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications.The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology-824, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands, a.vanrijk@pathol.umcn.nl.

ABSTRACT
Lymphomas originating from the lymphatic system comprise about 30 entities classified according to the World Health Organization (WHO). The histopathological diagnosis is generally considered difficult and prone to mistakes. Since non-random chromosomal translocations are specifically involved in different lymphoma entities, their detection will be increasingly important. Hence, a split-signal fluorescence in situ hybridisation (FISH) procedure would be helpful in discriminating the most difficult classifications. The Euro-FISH programme, a concerted action of nine European laboratories, has validated a robust, standardised protocol to improve the diagnostic approach on lymphoma entities. Therefore, 16 fluorescent probes and 10 WHO entities, supplemented with reactive cases, were selected. The results of the Euro-FISH programme show that all probes were correctly cytogenetically located, that the standardised protocol is robust, resulting in reliable results in approximately 90% of cases, and that the procedure could be implemented in every laboratory, bringing the relatively easy interpretation of split-signal probes within the reach of many pathology laboratories.

No MeSH data available.


Related in: MedlinePlus