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Comparative chromosome mapping of repetitive sequences. Implications for genomic evolution in the fish, Hoplias malabaricus.

Cioffi MB, Martins C, Bertollo LA - BMC Genet. (2009)

Bottom Line: The results showed that several chromosomal markers had conserved location in the four karyomorphs.These data therefore reinforced and confirmed the proposed grouping of karyomorphs A-D in Group I and highlight a closer relationship between karyomorphs A-B and C-D.Moreover, the mapping pattern of some markers on some autosomes and on the chromosomes of the XY and X1X2Y systems provided new evidence concerning the possible origin of the sex chromosomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Universidade Federal de São Carlos, Departamento de Genética e Evolução, São Carlos, SP, Brazil. mbello.ufscar@gmail.com

ABSTRACT

Background: Seven karyomorphs of the fish, Hoplias malabaricus (A-G) were previously included in two major groups, Group I (A, B, C, D) and Group II (E, F, G), based on their similar karyotype structure. In this paper, karyomorphs from Group I were analyzed by means of distinct chromosomal markers, including silver-stained nucleolar organizer regions (Ag-NORs) and chromosomal location of repetitive sequences (18S and 5S rDNA, and satellite 5SHindIII-DNA), through fluorescence in situ hybridization (FISH), in order to evaluate the evolutionary relationships among them.

Results: The results showed that several chromosomal markers had conserved location in the four karyomorphs. In addition, some other markers were only conserved in corresponding chromosomes of karyomorphs A-B and C-D. These data therefore reinforced and confirmed the proposed grouping of karyomorphs A-D in Group I and highlight a closer relationship between karyomorphs A-B and C-D. Moreover, the mapping pattern of some markers on some autosomes and on the chromosomes of the XY and X1X2Y systems provided new evidence concerning the possible origin of the sex chromosomes.

Conclusion: The in situ investigation of repetitive DNA sequences adds new informative characters useful in comparative genomics at chromosomal level and provides insights into the evolutionary relationships among Hoplias malabaricus karyomorphs.

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Related in: MedlinePlus

Karyotypes of Hoplias malabaricus (karyomorphs A-D) arranged from chromosomes probed with 5SHindIII-DNA satellite sequences (yellow signals) and counterstained with propidium iodide. The sex chromosomes of karyomorphs B and D are boxed. Bar = 5 μm.
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Figure 1: Karyotypes of Hoplias malabaricus (karyomorphs A-D) arranged from chromosomes probed with 5SHindIII-DNA satellite sequences (yellow signals) and counterstained with propidium iodide. The sex chromosomes of karyomorphs B and D are boxed. Bar = 5 μm.

Mentions: Sites of 5SHindIII-DNA, 5S rDNA and 18S rDNA were clearly detected by the FISH procedures, allowing their clear identification and location in the chromosomes of H. malabaricus (Figures 1, 2, 3 and 4). These data were organized in the form of idiograms (Figure 5) to facilitate the comparative analysis between the karyomorphs.


Comparative chromosome mapping of repetitive sequences. Implications for genomic evolution in the fish, Hoplias malabaricus.

Cioffi MB, Martins C, Bertollo LA - BMC Genet. (2009)

Karyotypes of Hoplias malabaricus (karyomorphs A-D) arranged from chromosomes probed with 5SHindIII-DNA satellite sequences (yellow signals) and counterstained with propidium iodide. The sex chromosomes of karyomorphs B and D are boxed. Bar = 5 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713275&req=5

Figure 1: Karyotypes of Hoplias malabaricus (karyomorphs A-D) arranged from chromosomes probed with 5SHindIII-DNA satellite sequences (yellow signals) and counterstained with propidium iodide. The sex chromosomes of karyomorphs B and D are boxed. Bar = 5 μm.
Mentions: Sites of 5SHindIII-DNA, 5S rDNA and 18S rDNA were clearly detected by the FISH procedures, allowing their clear identification and location in the chromosomes of H. malabaricus (Figures 1, 2, 3 and 4). These data were organized in the form of idiograms (Figure 5) to facilitate the comparative analysis between the karyomorphs.

Bottom Line: The results showed that several chromosomal markers had conserved location in the four karyomorphs.These data therefore reinforced and confirmed the proposed grouping of karyomorphs A-D in Group I and highlight a closer relationship between karyomorphs A-B and C-D.Moreover, the mapping pattern of some markers on some autosomes and on the chromosomes of the XY and X1X2Y systems provided new evidence concerning the possible origin of the sex chromosomes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Universidade Federal de São Carlos, Departamento de Genética e Evolução, São Carlos, SP, Brazil. mbello.ufscar@gmail.com

ABSTRACT

Background: Seven karyomorphs of the fish, Hoplias malabaricus (A-G) were previously included in two major groups, Group I (A, B, C, D) and Group II (E, F, G), based on their similar karyotype structure. In this paper, karyomorphs from Group I were analyzed by means of distinct chromosomal markers, including silver-stained nucleolar organizer regions (Ag-NORs) and chromosomal location of repetitive sequences (18S and 5S rDNA, and satellite 5SHindIII-DNA), through fluorescence in situ hybridization (FISH), in order to evaluate the evolutionary relationships among them.

Results: The results showed that several chromosomal markers had conserved location in the four karyomorphs. In addition, some other markers were only conserved in corresponding chromosomes of karyomorphs A-B and C-D. These data therefore reinforced and confirmed the proposed grouping of karyomorphs A-D in Group I and highlight a closer relationship between karyomorphs A-B and C-D. Moreover, the mapping pattern of some markers on some autosomes and on the chromosomes of the XY and X1X2Y systems provided new evidence concerning the possible origin of the sex chromosomes.

Conclusion: The in situ investigation of repetitive DNA sequences adds new informative characters useful in comparative genomics at chromosomal level and provides insights into the evolutionary relationships among Hoplias malabaricus karyomorphs.

Show MeSH
Related in: MedlinePlus