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Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH
Sequence alignment of La1 like peptides. SJEs are clusters from this work. The others are P0C5F3 (Venom peptide La1, Liocheles australasiae), Q4PMM0 (Putative secreted salivary protein, Ixodes scapularis), and Q95P92(TXLP1, Mesobuthus martensii).
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Figure 8: Sequence alignment of La1 like peptides. SJEs are clusters from this work. The others are P0C5F3 (Venom peptide La1, Liocheles australasiae), Q4PMM0 (Putative secreted salivary protein, Ixodes scapularis), and Q95P92(TXLP1, Mesobuthus martensii).

Mentions: La1 is the most abundant venom peptide obtained from the scorpion Liocheles australasiae[4], which was once considered to be a member of the family Hemiscorpiidae, but now has been classified into the family Ischnuridae[1]. Acturally, this type of venom peptides was firstly characterized from the scorpion Mesobuthus martensii at the transcript level. Until now, there have been no clues to their biological function. This work revealed six clusters of La1-like peptides, including four contigs and two singletons (Figure 8). In terms of primary sequence similarity and the position of eight cysteines, they are homologous to several known peptides, including secretory peptides from the salivary gland of Ixodes scapularis ticks[49]. This demonstrates that La1-like peptides have an ancient origin[50].


Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Sequence alignment of La1 like peptides. SJEs are clusters from this work. The others are P0C5F3 (Venom peptide La1, Liocheles australasiae), Q4PMM0 (Putative secreted salivary protein, Ixodes scapularis), and Q95P92(TXLP1, Mesobuthus martensii).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713264&req=5

Figure 8: Sequence alignment of La1 like peptides. SJEs are clusters from this work. The others are P0C5F3 (Venom peptide La1, Liocheles australasiae), Q4PMM0 (Putative secreted salivary protein, Ixodes scapularis), and Q95P92(TXLP1, Mesobuthus martensii).
Mentions: La1 is the most abundant venom peptide obtained from the scorpion Liocheles australasiae[4], which was once considered to be a member of the family Hemiscorpiidae, but now has been classified into the family Ischnuridae[1]. Acturally, this type of venom peptides was firstly characterized from the scorpion Mesobuthus martensii at the transcript level. Until now, there have been no clues to their biological function. This work revealed six clusters of La1-like peptides, including four contigs and two singletons (Figure 8). In terms of primary sequence similarity and the position of eight cysteines, they are homologous to several known peptides, including secretory peptides from the salivary gland of Ixodes scapularis ticks[49]. This demonstrates that La1-like peptides have an ancient origin[50].

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH