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Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH
Sequence alignment of trypsin inhibitor like (TIL) peptides. SJEs are clusters from this work. The others are Q17PK3 (Cysteine-rich venom protein, Aedes aegypti), Q1HRK9 (TIL domain-containing cysteine-rich salivary secreted peptide, Aedes aegypti), and Q86RQ7 (Venom peptide BmKAPi, Mesobuthus martensii).
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Figure 6: Sequence alignment of trypsin inhibitor like (TIL) peptides. SJEs are clusters from this work. The others are Q17PK3 (Cysteine-rich venom protein, Aedes aegypti), Q1HRK9 (TIL domain-containing cysteine-rich salivary secreted peptide, Aedes aegypti), and Q86RQ7 (Venom peptide BmKAPi, Mesobuthus martensii).

Mentions: A trypsin inhibitor like venom peptide, BmKAPi, has previously been characterized from the scorpion Mesobuthus martensii[38]. The trypsin inhibitor like domain (Pfam: PF01826) contains ten cysteine residues that form five disulphide bonds[39]. However, the exact function of trypsin inhibitor like peptides from scorpion venoms hasn't been clarified[40]. Four clusters (three contigs and one singleton, 15 ESTs) were identified to encode trypsin inhibitor like peptides (Figure 6).


Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Sequence alignment of trypsin inhibitor like (TIL) peptides. SJEs are clusters from this work. The others are Q17PK3 (Cysteine-rich venom protein, Aedes aegypti), Q1HRK9 (TIL domain-containing cysteine-rich salivary secreted peptide, Aedes aegypti), and Q86RQ7 (Venom peptide BmKAPi, Mesobuthus martensii).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713264&req=5

Figure 6: Sequence alignment of trypsin inhibitor like (TIL) peptides. SJEs are clusters from this work. The others are Q17PK3 (Cysteine-rich venom protein, Aedes aegypti), Q1HRK9 (TIL domain-containing cysteine-rich salivary secreted peptide, Aedes aegypti), and Q86RQ7 (Venom peptide BmKAPi, Mesobuthus martensii).
Mentions: A trypsin inhibitor like venom peptide, BmKAPi, has previously been characterized from the scorpion Mesobuthus martensii[38]. The trypsin inhibitor like domain (Pfam: PF01826) contains ten cysteine residues that form five disulphide bonds[39]. However, the exact function of trypsin inhibitor like peptides from scorpion venoms hasn't been clarified[40]. Four clusters (three contigs and one singleton, 15 ESTs) were identified to encode trypsin inhibitor like peptides (Figure 6).

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH