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Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH
Sequence alignment of α-KTxs. SJEs are clusters from this work. The others are Q6XLL5 (alpha-KTx 6.10, Opistophthalmus carinatus), and A9QLM3 (LmKTx8, Lychas mucronatus).
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Figure 2: Sequence alignment of α-KTxs. SJEs are clusters from this work. The others are Q6XLL5 (alpha-KTx 6.10, Opistophthalmus carinatus), and A9QLM3 (LmKTx8, Lychas mucronatus).

Mentions: α-KTxs have a wide phylogenetic distribution, and have been obtained from almost all scorpion species studied so far[8]. The newly identified α-KTxs are encoded by seven clusters (six contigs and one singleton, 45 ESTs). Among them, three clusters (SJE076C, SJE093C and SJE094C) code for α-KTxs contrained by 3 disulfide bridges, whereas the other clusters for α-KTxs with four disulfide bridges (Figure 2). For some toxins, the fourth disulfide bridge is of great importance in reaching the correct bioactive conformation[18]. They share the Toxin_2 domain (Pfam: PF00451) with other previously characterized scorpion short-chain toxins which could act on shaker-related channels or Ca2+-activated K+-channels[8].


Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Sequence alignment of α-KTxs. SJEs are clusters from this work. The others are Q6XLL5 (alpha-KTx 6.10, Opistophthalmus carinatus), and A9QLM3 (LmKTx8, Lychas mucronatus).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713264&req=5

Figure 2: Sequence alignment of α-KTxs. SJEs are clusters from this work. The others are Q6XLL5 (alpha-KTx 6.10, Opistophthalmus carinatus), and A9QLM3 (LmKTx8, Lychas mucronatus).
Mentions: α-KTxs have a wide phylogenetic distribution, and have been obtained from almost all scorpion species studied so far[8]. The newly identified α-KTxs are encoded by seven clusters (six contigs and one singleton, 45 ESTs). Among them, three clusters (SJE076C, SJE093C and SJE094C) code for α-KTxs contrained by 3 disulfide bridges, whereas the other clusters for α-KTxs with four disulfide bridges (Figure 2). For some toxins, the fourth disulfide bridge is of great importance in reaching the correct bioactive conformation[18]. They share the Toxin_2 domain (Pfam: PF00451) with other previously characterized scorpion short-chain toxins which could act on shaker-related channels or Ca2+-activated K+-channels[8].

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH