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Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

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ESTs distribution by cluster size. For instance, there are 6 clusters of size 6, accounting for a sum of 36 ESTs.
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Figure 1: ESTs distribution by cluster size. For instance, there are 6 clusters of size 6, accounting for a sum of 36 ESTs.

Mentions: The titer of the non-amplified cDNA library is 3.5 × 106 cfu/ml with more than 98% recombination efficiency. The random sequencing of this library gave readable sequences for a total of 871 clones. After being processed as described in the "Materials and Methods" section, the high quality EST sequences were submitted into the dbEST (accession numbers: GH547439–GH548309). The average length of these processed sequences was 546 bp. After being grouped with stringent parameters, the ESTs formed 293 clusters of unique sequences, including 199 singletons and 94 contigs consisting of two or more ESTs (Figure 1). In this study, the terms "contig" and "singleton" were used as the same meaning as described in Egassembler[17].


Transcriptome analysis of the venom gland of the scorpion Scorpiops jendeki: implication for the evolution of the scorpion venom arsenal.

Ma Y, Zhao R, He Y, Li S, Liu J, Wu Y, Cao Z, Li W - BMC Genomics (2009)

ESTs distribution by cluster size. For instance, there are 6 clusters of size 6, accounting for a sum of 36 ESTs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713264&req=5

Figure 1: ESTs distribution by cluster size. For instance, there are 6 clusters of size 6, accounting for a sum of 36 ESTs.
Mentions: The titer of the non-amplified cDNA library is 3.5 × 106 cfu/ml with more than 98% recombination efficiency. The random sequencing of this library gave readable sequences for a total of 871 clones. After being processed as described in the "Materials and Methods" section, the high quality EST sequences were submitted into the dbEST (accession numbers: GH547439–GH548309). The average length of these processed sequences was 546 bp. After being grouped with stringent parameters, the ESTs formed 293 clusters of unique sequences, including 199 singletons and 94 contigs consisting of two or more ESTs (Figure 1). In this study, the terms "contig" and "singleton" were used as the same meaning as described in Egassembler[17].

Bottom Line: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion.This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins.Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, PR China. mayibao@163.com

ABSTRACT

Background: The family Euscorpiidae, which covers Europe, Asia, Africa, and America, is one of the most widely distributed scorpion groups. However, no studies have been conducted on the venom of a Euscorpiidae species yet. In this work, we performed a transcriptomic approach for characterizing the venom components from a Euscorpiidae scorpion, Scorpiops jendeki.

Results: There are ten known types of venom peptides and proteins obtained from Scorpiops jendeki. Great diversity is observed in primary sequences of most highly expressed types. The most highly expressed types are cytolytic peptides and serine proteases. Neurotoxins specific for sodium channels, which are major groups of venom components from Buthidae scorpions, are not detected in this study. In addition to those known types of venom peptides and proteins, we also obtain nine atypical types of venom molecules which haven't been observed in any other scorpion species studied to date.

Conclusion: This work provides the first set of cDNAs from Scorpiops jendeki, and one of the few transcriptomic analyses from a scorpion. This allows the characterization of a large number of venom molecules, belonging to either known or atypical types of scorpion venom peptides and proteins. Besides, our work could provide some clues to the evolution of the scorpion venom arsenal by comparison with venom data from other scorpion lineages.

Show MeSH