Limits...
Anti-malarial activity of 6-(8'Z-pentadecenyl)-salicylic acid from Viola websteri in mice.

Chung IM, Seo SH, Kang EY, Park WH, Moon HI - Malar. J. (2009)

Bottom Line: Petroleum ether extracts of Viola websteri Hemsl (Violaceae) were reported to have anti-plasmodial activity against Plasmodium falciparum in vitro, with this activity being largely attributable to 6-(8'Z-pentadecenyl)-salicylic acid (6-SA).The possible 'repository' activity of 6-SA was assessed using the method described by Peters.In the present study, 6-SA was found to have anti-malarial activity in vivo, when tested against P. berghei in mice. 6-SA at 5, 10 and 25 mg/kg x day exhibited a significant blood schizontocidal activity in four-day early infections, repository evaluations and established infections with a significant mean survival time comparable to that of the standard drug, chloroquine (5 mg/kg.day). 6-SA possesses a moderate anti-malarial activity that could be exploited for malaria therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Diagnostics, College of Korean Medicine, Dongguk University, Gyeong-Ju 780-714, South Korea. himun68@daum.net

ABSTRACT

Background: Petroleum ether extracts of Viola websteri Hemsl (Violaceae) were reported to have anti-plasmodial activity against Plasmodium falciparum in vitro, with this activity being largely attributable to 6-(8'Z-pentadecenyl)-salicylic acid (6-SA).

Methods: The schizontocidal activity of 6-SA on early Plasmodium berghei infections was evaluated in a four-day test. The possible 'repository' activity of 6-SA was assessed using the method described by Peters. The median lethal dose (LD50) of 6-SA, when given intraperitoneally, was also determined using uninfected ICR mice and the method of Lorke.

Results: In the present study, 6-SA was found to have anti-malarial activity in vivo, when tested against P. berghei in mice. 6-SA at 5, 10 and 25 mg/kg x day exhibited a significant blood schizontocidal activity in four-day early infections, repository evaluations and established infections with a significant mean survival time comparable to that of the standard drug, chloroquine (5 mg/kg.day).

Conclusion: 6-SA possesses a moderate anti-malarial activity that could be exploited for malaria therapy.

Show MeSH

Related in: MedlinePlus

Structure of 6-SA isolated from Viola websteri Hemsl.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2713261&req=5

Figure 1: Structure of 6-SA isolated from Viola websteri Hemsl.

Mentions: Malaria is a major tropical disease caused by parasites and is responsible for significant morbidity and mortality worldwide [1]. There is currently a dramatic resurgence of the disease because of the increasing resistance of the vectors to insecticides and the progressive resistance of the causative parasites, particularly Plasmodium falciparum, to anti-malarial drugs [2]. Therefore, there is an urgent need to discover and develop new, effective and safe drugs for the treatment of this disease [3]. Malarial parasites and plant plastids have several common pathways and functions that are fundamentally different from the analogous pathways and functions in humans and may therefore make good targets for anti-malarial drugs [4]. In traditional medicine, Viola is used for the treatment of insect infections, cancer, virus infection, and skin diseases [5]. A previous study [6] revealed that the petroleum ether extracts from dried whole parts of the 15 Viola genera screened, Viola websteri had inhibition values of 31.7 as percentage of parasite inhibition at 25 μg/ml. Petroleum ether extracts from Viola websteri were found to have anti-plasmodial activity against P. falciparum in vitro, with this activity being largely attributable to 6-SA (Figure 1)[7]. In the present study, 6-SA was found to have anti-malarial activity in vivo when tested against Plasmodium berghei in mice.


Anti-malarial activity of 6-(8'Z-pentadecenyl)-salicylic acid from Viola websteri in mice.

Chung IM, Seo SH, Kang EY, Park WH, Moon HI - Malar. J. (2009)

Structure of 6-SA isolated from Viola websteri Hemsl.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713261&req=5

Figure 1: Structure of 6-SA isolated from Viola websteri Hemsl.
Mentions: Malaria is a major tropical disease caused by parasites and is responsible for significant morbidity and mortality worldwide [1]. There is currently a dramatic resurgence of the disease because of the increasing resistance of the vectors to insecticides and the progressive resistance of the causative parasites, particularly Plasmodium falciparum, to anti-malarial drugs [2]. Therefore, there is an urgent need to discover and develop new, effective and safe drugs for the treatment of this disease [3]. Malarial parasites and plant plastids have several common pathways and functions that are fundamentally different from the analogous pathways and functions in humans and may therefore make good targets for anti-malarial drugs [4]. In traditional medicine, Viola is used for the treatment of insect infections, cancer, virus infection, and skin diseases [5]. A previous study [6] revealed that the petroleum ether extracts from dried whole parts of the 15 Viola genera screened, Viola websteri had inhibition values of 31.7 as percentage of parasite inhibition at 25 μg/ml. Petroleum ether extracts from Viola websteri were found to have anti-plasmodial activity against P. falciparum in vitro, with this activity being largely attributable to 6-SA (Figure 1)[7]. In the present study, 6-SA was found to have anti-malarial activity in vivo when tested against Plasmodium berghei in mice.

Bottom Line: Petroleum ether extracts of Viola websteri Hemsl (Violaceae) were reported to have anti-plasmodial activity against Plasmodium falciparum in vitro, with this activity being largely attributable to 6-(8'Z-pentadecenyl)-salicylic acid (6-SA).The possible 'repository' activity of 6-SA was assessed using the method described by Peters.In the present study, 6-SA was found to have anti-malarial activity in vivo, when tested against P. berghei in mice. 6-SA at 5, 10 and 25 mg/kg x day exhibited a significant blood schizontocidal activity in four-day early infections, repository evaluations and established infections with a significant mean survival time comparable to that of the standard drug, chloroquine (5 mg/kg.day). 6-SA possesses a moderate anti-malarial activity that could be exploited for malaria therapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Diagnostics, College of Korean Medicine, Dongguk University, Gyeong-Ju 780-714, South Korea. himun68@daum.net

ABSTRACT

Background: Petroleum ether extracts of Viola websteri Hemsl (Violaceae) were reported to have anti-plasmodial activity against Plasmodium falciparum in vitro, with this activity being largely attributable to 6-(8'Z-pentadecenyl)-salicylic acid (6-SA).

Methods: The schizontocidal activity of 6-SA on early Plasmodium berghei infections was evaluated in a four-day test. The possible 'repository' activity of 6-SA was assessed using the method described by Peters. The median lethal dose (LD50) of 6-SA, when given intraperitoneally, was also determined using uninfected ICR mice and the method of Lorke.

Results: In the present study, 6-SA was found to have anti-malarial activity in vivo, when tested against P. berghei in mice. 6-SA at 5, 10 and 25 mg/kg x day exhibited a significant blood schizontocidal activity in four-day early infections, repository evaluations and established infections with a significant mean survival time comparable to that of the standard drug, chloroquine (5 mg/kg.day).

Conclusion: 6-SA possesses a moderate anti-malarial activity that could be exploited for malaria therapy.

Show MeSH
Related in: MedlinePlus