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Assess suitability of hydroaeroponic culture to establish tripartite symbiosis between different AMF species, beans, and rhizobia.

Tajini F, Suriyakup P, Vailhe H, Jansa J, Drevon JJ - BMC Plant Biol. (2009)

Bottom Line: Both rhizobial and mycorrhizal symbioses can act synergistically in benefits on plant.The effect of bean genotype on both rhizobial and mycorrhizal symbioses with Glomus was subsequently assessed with the common bean recombinant inbreed line 7, 28, 83, 115 and 147, and the cultivar Flamingo.Significant differences among colonization and nodulation of the roots and growth among genotypes were found.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut National de Recherche Agronomique, UMR1222 Ecologie Fonctionnelle & Biogéochimie des Sols, INRA-IRD-SupAgro, 2 place Viala, 34060 Montpellier Cedex, France. fatmatajini@yahoo.fr

ABSTRACT

Background: Like other species of the Phaseoleae tribe, common bean (Phaseolus vulgaris L.) has the potential to establish symbiosis with rhizobia and to fix the atmospheric dinitrogen (N2) for its N nutrition. Common bean has also the potential to establish symbiosis with arbuscular mycorrhizal fungi (AMF) that improves the uptake of low mobile nutrients such as phosphorus, from the soil. Both rhizobial and mycorrhizal symbioses can act synergistically in benefits on plant.

Results: The tripartite symbiosis of common bean with rhizobia and arbuscular mycorrhizal fungi (AMF) was assessed in hydroaeroponic culture with common bean (Phaseolus vulgaris L.), by comparing the effects of three fungi spp. on growth, nodulation and mycorrhization of the roots under sufficient versus deficient P supplies, after transfer from initial sand culture. Although Glomus intraradices Schenck & Smith colonized intensely the roots of common bean in both sand and hydroaeroponic cultures, Gigaspora rosea Nicolson & Schenck only established well under sand culture conditions, and no root-colonization was found with Acaulospora mellea Spain & Schenck under either culture conditions. Interestingly, mycorrhization by Glomus was also obtained by contact with mycorrhized Stylosanthes guianensis (Aubl.) sw in sand culture under deficient P before transfer into hydroaeroponic culture. The effect of bean genotype on both rhizobial and mycorrhizal symbioses with Glomus was subsequently assessed with the common bean recombinant inbreed line 7, 28, 83, 115 and 147, and the cultivar Flamingo. Significant differences among colonization and nodulation of the roots and growth among genotypes were found.

Conclusion: The hydroaeroponic culture is a valuable tool for further scrutinizing the physiological interactions and nutrient partitioning within the tripartite symbiosis.

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Related in: MedlinePlus

Effect of Glomus, Gigaspora and Acaulospora on: shoot phosphorus content (%) and phosphorus use efficiency of common bean. Common bean genotype 115 (A and A') inoculated with Glomus, Gigaspora and Acaulospora, and recombinant inbred genotypes 115, 147, 83, 7, 28 and Flamingo (B and B') inoculated with Glomus, grown in hydroaeroponic culture under P sufficiency (open bars) versus P deficiency (grey bars) or in sand culture (black bars). Data are means ± SD of three replicates harvested at 50 days after sowing. For each cultivation system, different letters indicate significant differences between treatment means.
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Figure 4: Effect of Glomus, Gigaspora and Acaulospora on: shoot phosphorus content (%) and phosphorus use efficiency of common bean. Common bean genotype 115 (A and A') inoculated with Glomus, Gigaspora and Acaulospora, and recombinant inbred genotypes 115, 147, 83, 7, 28 and Flamingo (B and B') inoculated with Glomus, grown in hydroaeroponic culture under P sufficiency (open bars) versus P deficiency (grey bars) or in sand culture (black bars). Data are means ± SD of three replicates harvested at 50 days after sowing. For each cultivation system, different letters indicate significant differences between treatment means.

Mentions: Phosphorus content of the shoot of RIL115 was affected by the cultivation system (p < 0.001), but not by AMF species identity (p = 0.23) nor by the interaction of the two factors (p = 0.48) in the 1st experiment. Phosphorus content in hydroaeroponics under P sufficiency compared to sand was nearly 3 fold higher with Glomus (0.50% ± 0.12 versus 0.17% ± 0.02), 2.7 fold higher with Gigaspora (0.48% ± 0.11 versus 0.17% ± 0.02) and 2 fold higher with Acaulospora (0.30% ± 0.08 versus 0.14% ± 0.04) (Fig. 4A). Under P deficiency the phosphorus content was reduced by half with Glomus and Gigaspora (Fig. 4A). As a consequence, the P use efficiency (PUE) was strongly affected by the cultivation system (p < 0.001) and by AMF species identity (p < 0.05) but no by the interaction of the two factors (p = 0.61). The P use efficiency was 2 and 3 fold higher in hydroaeroponics under P deficiency than in sand, and higher with Glomus (0.45 ± 0.12 g DW mg-1 P) than with Gigaspora or Acaulospora (0.36 ± 0.04 or 0.30 ± 0.11 g DW mg-1 P) under P deficiency (Fig. 4A').


Assess suitability of hydroaeroponic culture to establish tripartite symbiosis between different AMF species, beans, and rhizobia.

Tajini F, Suriyakup P, Vailhe H, Jansa J, Drevon JJ - BMC Plant Biol. (2009)

Effect of Glomus, Gigaspora and Acaulospora on: shoot phosphorus content (%) and phosphorus use efficiency of common bean. Common bean genotype 115 (A and A') inoculated with Glomus, Gigaspora and Acaulospora, and recombinant inbred genotypes 115, 147, 83, 7, 28 and Flamingo (B and B') inoculated with Glomus, grown in hydroaeroponic culture under P sufficiency (open bars) versus P deficiency (grey bars) or in sand culture (black bars). Data are means ± SD of three replicates harvested at 50 days after sowing. For each cultivation system, different letters indicate significant differences between treatment means.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2713235&req=5

Figure 4: Effect of Glomus, Gigaspora and Acaulospora on: shoot phosphorus content (%) and phosphorus use efficiency of common bean. Common bean genotype 115 (A and A') inoculated with Glomus, Gigaspora and Acaulospora, and recombinant inbred genotypes 115, 147, 83, 7, 28 and Flamingo (B and B') inoculated with Glomus, grown in hydroaeroponic culture under P sufficiency (open bars) versus P deficiency (grey bars) or in sand culture (black bars). Data are means ± SD of three replicates harvested at 50 days after sowing. For each cultivation system, different letters indicate significant differences between treatment means.
Mentions: Phosphorus content of the shoot of RIL115 was affected by the cultivation system (p < 0.001), but not by AMF species identity (p = 0.23) nor by the interaction of the two factors (p = 0.48) in the 1st experiment. Phosphorus content in hydroaeroponics under P sufficiency compared to sand was nearly 3 fold higher with Glomus (0.50% ± 0.12 versus 0.17% ± 0.02), 2.7 fold higher with Gigaspora (0.48% ± 0.11 versus 0.17% ± 0.02) and 2 fold higher with Acaulospora (0.30% ± 0.08 versus 0.14% ± 0.04) (Fig. 4A). Under P deficiency the phosphorus content was reduced by half with Glomus and Gigaspora (Fig. 4A). As a consequence, the P use efficiency (PUE) was strongly affected by the cultivation system (p < 0.001) and by AMF species identity (p < 0.05) but no by the interaction of the two factors (p = 0.61). The P use efficiency was 2 and 3 fold higher in hydroaeroponics under P deficiency than in sand, and higher with Glomus (0.45 ± 0.12 g DW mg-1 P) than with Gigaspora or Acaulospora (0.36 ± 0.04 or 0.30 ± 0.11 g DW mg-1 P) under P deficiency (Fig. 4A').

Bottom Line: Both rhizobial and mycorrhizal symbioses can act synergistically in benefits on plant.The effect of bean genotype on both rhizobial and mycorrhizal symbioses with Glomus was subsequently assessed with the common bean recombinant inbreed line 7, 28, 83, 115 and 147, and the cultivar Flamingo.Significant differences among colonization and nodulation of the roots and growth among genotypes were found.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut National de Recherche Agronomique, UMR1222 Ecologie Fonctionnelle & Biogéochimie des Sols, INRA-IRD-SupAgro, 2 place Viala, 34060 Montpellier Cedex, France. fatmatajini@yahoo.fr

ABSTRACT

Background: Like other species of the Phaseoleae tribe, common bean (Phaseolus vulgaris L.) has the potential to establish symbiosis with rhizobia and to fix the atmospheric dinitrogen (N2) for its N nutrition. Common bean has also the potential to establish symbiosis with arbuscular mycorrhizal fungi (AMF) that improves the uptake of low mobile nutrients such as phosphorus, from the soil. Both rhizobial and mycorrhizal symbioses can act synergistically in benefits on plant.

Results: The tripartite symbiosis of common bean with rhizobia and arbuscular mycorrhizal fungi (AMF) was assessed in hydroaeroponic culture with common bean (Phaseolus vulgaris L.), by comparing the effects of three fungi spp. on growth, nodulation and mycorrhization of the roots under sufficient versus deficient P supplies, after transfer from initial sand culture. Although Glomus intraradices Schenck & Smith colonized intensely the roots of common bean in both sand and hydroaeroponic cultures, Gigaspora rosea Nicolson & Schenck only established well under sand culture conditions, and no root-colonization was found with Acaulospora mellea Spain & Schenck under either culture conditions. Interestingly, mycorrhization by Glomus was also obtained by contact with mycorrhized Stylosanthes guianensis (Aubl.) sw in sand culture under deficient P before transfer into hydroaeroponic culture. The effect of bean genotype on both rhizobial and mycorrhizal symbioses with Glomus was subsequently assessed with the common bean recombinant inbreed line 7, 28, 83, 115 and 147, and the cultivar Flamingo. Significant differences among colonization and nodulation of the roots and growth among genotypes were found.

Conclusion: The hydroaeroponic culture is a valuable tool for further scrutinizing the physiological interactions and nutrient partitioning within the tripartite symbiosis.

Show MeSH
Related in: MedlinePlus