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Anaplasma phagocytophilum and Anaplasma marginale elicit different gene expression responses in cultured tick cells.

Zivkovic Z, Blouin EF, Manzano-Roman R, Almazán C, Naranjo V, Massung RF, Jongejan F, Kocan KM, de la Fuente J - Comp. Funct. Genomics (2009)

Bottom Line: The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms, Anaplasma phagocytophilum and Anaplasma marginale that multiply in both vertebrate and tick host cells.The results of these studies demonstrated modulation of tick gene expression by A. phagocytophilum and provided evidence of different gene expression responses in tick cells infected with A. phagocytophilum and A. marginale.These differences in Anaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.

View Article: PubMed Central - PubMed

Affiliation: Utrecht Centre for Tick-Borne Diseases (UCTD), Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.

ABSTRACT
The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms, Anaplasma phagocytophilum and Anaplasma marginale that multiply in both vertebrate and tick host cells. Recently, we showed that A. marginale affects the expression of tick genes that are involved in tick survival and pathogen infection and multiplication. However, the gene expression profile in A. phagocytophilum-infected tick cells is currently poorly characterized. The objectives of this study were to characterize tick gene expression profile in Ixodes scapularis ticks and cultured ISE6 cells in response to infection with A. phagocypthilum and to compare tick gene expression responses in A. phagocytophilum- and A. marginale-infected tick cells by microarray and real-time RT-PCR analyses. The results of these studies demonstrated modulation of tick gene expression by A. phagocytophilum and provided evidence of different gene expression responses in tick cells infected with A. phagocytophilum and A. marginale. These differences in Anaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.

No MeSH data available.


Related in: MedlinePlus

Differential gene expression in A. phagocytophilum-infected I. scapularis ticks and cultured tick ISE6 cells. Real-time RT-PCR was done on uninfected and infected I. scapularis nymphs (three groups each of uninfected ticks, infected ticks (Gaillard strain; black bars) and infected ticks (Dawson strain; white bars) with 10 nymphs each) and uninfected and NY18 isolate-infected tick ISE6 cells (three independent cultures each; red bars). Bars represent the ratio between infected normalized Ct values/uninfected average normalized Ct values (+SD). The mRNA levels were normalized against tick β-actin (ACT) and compared between infected and uninfected ticks and tick cells by Student's t-test (*P ≤ .05).
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fig1: Differential gene expression in A. phagocytophilum-infected I. scapularis ticks and cultured tick ISE6 cells. Real-time RT-PCR was done on uninfected and infected I. scapularis nymphs (three groups each of uninfected ticks, infected ticks (Gaillard strain; black bars) and infected ticks (Dawson strain; white bars) with 10 nymphs each) and uninfected and NY18 isolate-infected tick ISE6 cells (three independent cultures each; red bars). Bars represent the ratio between infected normalized Ct values/uninfected average normalized Ct values (+SD). The mRNA levels were normalized against tick β-actin (ACT) and compared between infected and uninfected ticks and tick cells by Student's t-test (*P ≤ .05).

Mentions: The mRNA levels of selected genes differentially expressed in A. phagocytophilum-infected tick ISE6 cells were evaluated by real-time RT-PCR in infected and uninfected cells (Figure 1). Similar to microarray hybridization results, the analysis of mRNA levels by real-time RT-PCR showed significant upregulation of C4B10 (von Willebrand factor) and R1E12 (ribosomal protein L32) and downregulation of C3B2 (aspartic protease) in tick ISE6 cells infected with A. phagocytophilum (Figure 1). The mRNA levels of genes identified previously as differentially expressed in tick IDE8 cells and ticks infected with A. marginale [9] were also evaluated by real-time RT-PCR in infected and uninfected tick ISE6 cells. The mRNA levels of genes differentially expressed in A. marginale-infected ISE6 cells were similar to those reported previously in infected IDE8 cells [9] and data not shown. The results in A. phagocytophilum-infected ISE6 cells showed that pathogen infection significantly upregulated the expression of U2A8 (signal sequence receptor delta), 1I5B9 (ixodegrin-2A RGD containing protein), and 1I4G12 (unknown function) and downregulated the expression of 2I3A7 (NADH-ubiquinoe oxidoreductase) and 1I1H6 (glutathione S-transferase (GST)) in tick ISE6 cells (Figure 1).


Anaplasma phagocytophilum and Anaplasma marginale elicit different gene expression responses in cultured tick cells.

Zivkovic Z, Blouin EF, Manzano-Roman R, Almazán C, Naranjo V, Massung RF, Jongejan F, Kocan KM, de la Fuente J - Comp. Funct. Genomics (2009)

Differential gene expression in A. phagocytophilum-infected I. scapularis ticks and cultured tick ISE6 cells. Real-time RT-PCR was done on uninfected and infected I. scapularis nymphs (three groups each of uninfected ticks, infected ticks (Gaillard strain; black bars) and infected ticks (Dawson strain; white bars) with 10 nymphs each) and uninfected and NY18 isolate-infected tick ISE6 cells (three independent cultures each; red bars). Bars represent the ratio between infected normalized Ct values/uninfected average normalized Ct values (+SD). The mRNA levels were normalized against tick β-actin (ACT) and compared between infected and uninfected ticks and tick cells by Student's t-test (*P ≤ .05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2712686&req=5

fig1: Differential gene expression in A. phagocytophilum-infected I. scapularis ticks and cultured tick ISE6 cells. Real-time RT-PCR was done on uninfected and infected I. scapularis nymphs (three groups each of uninfected ticks, infected ticks (Gaillard strain; black bars) and infected ticks (Dawson strain; white bars) with 10 nymphs each) and uninfected and NY18 isolate-infected tick ISE6 cells (three independent cultures each; red bars). Bars represent the ratio between infected normalized Ct values/uninfected average normalized Ct values (+SD). The mRNA levels were normalized against tick β-actin (ACT) and compared between infected and uninfected ticks and tick cells by Student's t-test (*P ≤ .05).
Mentions: The mRNA levels of selected genes differentially expressed in A. phagocytophilum-infected tick ISE6 cells were evaluated by real-time RT-PCR in infected and uninfected cells (Figure 1). Similar to microarray hybridization results, the analysis of mRNA levels by real-time RT-PCR showed significant upregulation of C4B10 (von Willebrand factor) and R1E12 (ribosomal protein L32) and downregulation of C3B2 (aspartic protease) in tick ISE6 cells infected with A. phagocytophilum (Figure 1). The mRNA levels of genes identified previously as differentially expressed in tick IDE8 cells and ticks infected with A. marginale [9] were also evaluated by real-time RT-PCR in infected and uninfected tick ISE6 cells. The mRNA levels of genes differentially expressed in A. marginale-infected ISE6 cells were similar to those reported previously in infected IDE8 cells [9] and data not shown. The results in A. phagocytophilum-infected ISE6 cells showed that pathogen infection significantly upregulated the expression of U2A8 (signal sequence receptor delta), 1I5B9 (ixodegrin-2A RGD containing protein), and 1I4G12 (unknown function) and downregulated the expression of 2I3A7 (NADH-ubiquinoe oxidoreductase) and 1I1H6 (glutathione S-transferase (GST)) in tick ISE6 cells (Figure 1).

Bottom Line: The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms, Anaplasma phagocytophilum and Anaplasma marginale that multiply in both vertebrate and tick host cells.The results of these studies demonstrated modulation of tick gene expression by A. phagocytophilum and provided evidence of different gene expression responses in tick cells infected with A. phagocytophilum and A. marginale.These differences in Anaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.

View Article: PubMed Central - PubMed

Affiliation: Utrecht Centre for Tick-Borne Diseases (UCTD), Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.

ABSTRACT
The genus Anaplasma (Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms, Anaplasma phagocytophilum and Anaplasma marginale that multiply in both vertebrate and tick host cells. Recently, we showed that A. marginale affects the expression of tick genes that are involved in tick survival and pathogen infection and multiplication. However, the gene expression profile in A. phagocytophilum-infected tick cells is currently poorly characterized. The objectives of this study were to characterize tick gene expression profile in Ixodes scapularis ticks and cultured ISE6 cells in response to infection with A. phagocypthilum and to compare tick gene expression responses in A. phagocytophilum- and A. marginale-infected tick cells by microarray and real-time RT-PCR analyses. The results of these studies demonstrated modulation of tick gene expression by A. phagocytophilum and provided evidence of different gene expression responses in tick cells infected with A. phagocytophilum and A. marginale. These differences in Anaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.

No MeSH data available.


Related in: MedlinePlus