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Successful birth of the first frozen oocyte baby in India.

Selvaraj P, Selvaraj K, Srinivasan K - J Hum Reprod Sci (2009)

Bottom Line: Embryos were generated by microinjection of frozen-thawed sperms into thawed human oocytes (intracytoplasmic sperm injection).This resulted in an healthy male baby with a birth weight of 2.54 kg which was born by cesarean section at 35-36 weeks of gestation with normal follow-up.Thus oocyte cryopreservation can be performed with reproducible success leading to a viable offspring.

View Article: PubMed Central - PubMed

Affiliation: Fertility Research Centre, G G Hospital, Chennai - 600 034, India.

ABSTRACT
We report the first pregnancy and birth in India after the transfer of embryos generated from frozen- thawed oocytes. A 29-year-old woman with previous bad obstetric history and an abnormal karyotype, necessitating donor oocyte programme. Embryos were generated by microinjection of frozen-thawed sperms into thawed human oocytes (intracytoplasmic sperm injection). This resulted in an healthy male baby with a birth weight of 2.54 kg which was born by cesarean section at 35-36 weeks of gestation with normal follow-up. Thus oocyte cryopreservation can be performed with reproducible success leading to a viable offspring.

No MeSH data available.


Intracytoplasmic sperm injection
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Figure 0004: Intracytoplasmic sperm injection

Mentions: A survival check carried out under the inverted microscope revealed eight oocytes that survived the thawing process [Figure 2]. Oocytes with intact zona, clear perivitelline space, and visible polar body were considered as normal and were further cultured in the Cleavage media (Quinn's, SAGE In vitro fertilization, Trumbull CT, USA) at 37°C and 5% CO2 for 2 h before injection. Subsequently, the oocytes were injected with frozen–thawed and prepared sample of husband's sperms. Intracytoplasmic sperm injection [Figures 3 and 4][4–9] was the preferred method for fertilization, owing to an expected zona hardening following oocyte cryopreservation. The injected oocytes were further cultured in cleavage media in a Trigas incubator until Pronucleus check 16–18h later. Five embryos (two and three cells, grade II–III) [Figures 5 and 6] were observed and transferred on Day 2 using a Labotect catheter (Labotect GmbH, Gottinggen, Germany). The assessment of embryo grading was performed as described by Veeck L.[10] Why five and not the recommended three embryos? Well, considering our previous experience of inadequate embryos for transfer following thawing and negative result for pregnancy, we decided to transfer at least five of this crop, which, as we mentioned, did not have the conventional cleavage or grade as expected (i.e., four cells, grade I–II). Once the serum β-HCG was positive, an ultrasound on the 38th day revealed a single intrauterine gestational sac. Since then she progressed uneventfully, with early fetal screening tests being normal until the 5th month, when she was diagnosed with mild pregnancy-induced hypertension and was treated with anti-hypertensives. During her level-2 scan at 22 weeks, where the fetal screening was normal, a shortened cervix of 1.5 cm was imaged, suggestive of cervical incompetence. Since then, she was admitted with us for careful surveillance of both the mother and the baby. She did moderate physical activities and her blood pressure was well controlled with anti-hypertensives. An elective cesarean was performed at 35–36 weeks on 27/8/2008 owing to severe oligohydraminos. She delivered a healthy male baby weighing 2.54 kg [Figure 7]. Both the mother and the baby were discharged uneventfully. A general follow-up at 3 months showed normal development for age [Figure 8].


Successful birth of the first frozen oocyte baby in India.

Selvaraj P, Selvaraj K, Srinivasan K - J Hum Reprod Sci (2009)

Intracytoplasmic sperm injection
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2700691&req=5

Figure 0004: Intracytoplasmic sperm injection
Mentions: A survival check carried out under the inverted microscope revealed eight oocytes that survived the thawing process [Figure 2]. Oocytes with intact zona, clear perivitelline space, and visible polar body were considered as normal and were further cultured in the Cleavage media (Quinn's, SAGE In vitro fertilization, Trumbull CT, USA) at 37°C and 5% CO2 for 2 h before injection. Subsequently, the oocytes were injected with frozen–thawed and prepared sample of husband's sperms. Intracytoplasmic sperm injection [Figures 3 and 4][4–9] was the preferred method for fertilization, owing to an expected zona hardening following oocyte cryopreservation. The injected oocytes were further cultured in cleavage media in a Trigas incubator until Pronucleus check 16–18h later. Five embryos (two and three cells, grade II–III) [Figures 5 and 6] were observed and transferred on Day 2 using a Labotect catheter (Labotect GmbH, Gottinggen, Germany). The assessment of embryo grading was performed as described by Veeck L.[10] Why five and not the recommended three embryos? Well, considering our previous experience of inadequate embryos for transfer following thawing and negative result for pregnancy, we decided to transfer at least five of this crop, which, as we mentioned, did not have the conventional cleavage or grade as expected (i.e., four cells, grade I–II). Once the serum β-HCG was positive, an ultrasound on the 38th day revealed a single intrauterine gestational sac. Since then she progressed uneventfully, with early fetal screening tests being normal until the 5th month, when she was diagnosed with mild pregnancy-induced hypertension and was treated with anti-hypertensives. During her level-2 scan at 22 weeks, where the fetal screening was normal, a shortened cervix of 1.5 cm was imaged, suggestive of cervical incompetence. Since then, she was admitted with us for careful surveillance of both the mother and the baby. She did moderate physical activities and her blood pressure was well controlled with anti-hypertensives. An elective cesarean was performed at 35–36 weeks on 27/8/2008 owing to severe oligohydraminos. She delivered a healthy male baby weighing 2.54 kg [Figure 7]. Both the mother and the baby were discharged uneventfully. A general follow-up at 3 months showed normal development for age [Figure 8].

Bottom Line: Embryos were generated by microinjection of frozen-thawed sperms into thawed human oocytes (intracytoplasmic sperm injection).This resulted in an healthy male baby with a birth weight of 2.54 kg which was born by cesarean section at 35-36 weeks of gestation with normal follow-up.Thus oocyte cryopreservation can be performed with reproducible success leading to a viable offspring.

View Article: PubMed Central - PubMed

Affiliation: Fertility Research Centre, G G Hospital, Chennai - 600 034, India.

ABSTRACT
We report the first pregnancy and birth in India after the transfer of embryos generated from frozen- thawed oocytes. A 29-year-old woman with previous bad obstetric history and an abnormal karyotype, necessitating donor oocyte programme. Embryos were generated by microinjection of frozen-thawed sperms into thawed human oocytes (intracytoplasmic sperm injection). This resulted in an healthy male baby with a birth weight of 2.54 kg which was born by cesarean section at 35-36 weeks of gestation with normal follow-up. Thus oocyte cryopreservation can be performed with reproducible success leading to a viable offspring.

No MeSH data available.