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Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae.

Zhang L, Zhu Z, Jing H, Zhang J, Xiong Y, Yan M, Gao S, Wu LF, Xu J, Kan B - BMC Microbiol. (2009)

Bottom Line: Deletion of the tatABC genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines.In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in tatABC mutants, suggesting an indirect effect of the mutation on toxin production.No obvious differences in flagellum biosynthesis and motility were found between the tatABC mutant and the parental strain, showing a variable effect of Tat in different bacteria.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Department of Diarrheal Diseases, Chinese Center for Disease Control and Prevention, Beijing, PR China. zhanglijuan@icdc.cn

ABSTRACT

Background: The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to Vibrio cholerae has not been explored. Here we investigated the functionality of the Tat system in V. cholerae, the etiologic agent of cholera.

Results: In V. cholerae, the tatABC genes function in the translocation of TMAO reductase. Deletion of the tatABC genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in tatABC mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the tatABC mutant and the parental strain, showing a variable effect of Tat in different bacteria.

Conclusion: The Tat system contributes to the survival of V. cholerae in the environment and in vivo, and it may be associated with its virulence.

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Related in: MedlinePlus

Comparison of biofilm formation by strains N16961 and N169-dtatABC cultured under aerobic and anaerobic conditions. For each strain (N16961 and n169-dtatABC), under each condition (aerobic and anaerobic), and at each time point, 7 wells were measured for repeat in one test. And the tests were repeated for three times. T-test was used for the comparison of strains N16961 and N169-dtatABC at each time point and under each condition. P values are less than 0.05 in all of the comparisons.
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Figure 5: Comparison of biofilm formation by strains N16961 and N169-dtatABC cultured under aerobic and anaerobic conditions. For each strain (N16961 and n169-dtatABC), under each condition (aerobic and anaerobic), and at each time point, 7 wells were measured for repeat in one test. And the tests were repeated for three times. T-test was used for the comparison of strains N16961 and N169-dtatABC at each time point and under each condition. P values are less than 0.05 in all of the comparisons.

Mentions: The ability to form biofilm formation is important for environmental survival and is a determining factor of virulence in pathogenic bacteria. To determine biofilm formation for the Tat mutants, we used a crystal violet staining method to quantify the adhering bacteria cultures in 96-well plates. Our findings indicate that under both aerobic and anaerobic conditions, the biofilm formation ability of the Tat mutant distinctly decreased (Fig. 5), which demonstrated that the Tat system of V. cholerae may play an important role in biofilm formation.


Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae.

Zhang L, Zhu Z, Jing H, Zhang J, Xiong Y, Yan M, Gao S, Wu LF, Xu J, Kan B - BMC Microbiol. (2009)

Comparison of biofilm formation by strains N16961 and N169-dtatABC cultured under aerobic and anaerobic conditions. For each strain (N16961 and n169-dtatABC), under each condition (aerobic and anaerobic), and at each time point, 7 wells were measured for repeat in one test. And the tests were repeated for three times. T-test was used for the comparison of strains N16961 and N169-dtatABC at each time point and under each condition. P values are less than 0.05 in all of the comparisons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2698830&req=5

Figure 5: Comparison of biofilm formation by strains N16961 and N169-dtatABC cultured under aerobic and anaerobic conditions. For each strain (N16961 and n169-dtatABC), under each condition (aerobic and anaerobic), and at each time point, 7 wells were measured for repeat in one test. And the tests were repeated for three times. T-test was used for the comparison of strains N16961 and N169-dtatABC at each time point and under each condition. P values are less than 0.05 in all of the comparisons.
Mentions: The ability to form biofilm formation is important for environmental survival and is a determining factor of virulence in pathogenic bacteria. To determine biofilm formation for the Tat mutants, we used a crystal violet staining method to quantify the adhering bacteria cultures in 96-well plates. Our findings indicate that under both aerobic and anaerobic conditions, the biofilm formation ability of the Tat mutant distinctly decreased (Fig. 5), which demonstrated that the Tat system of V. cholerae may play an important role in biofilm formation.

Bottom Line: Deletion of the tatABC genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines.In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in tatABC mutants, suggesting an indirect effect of the mutation on toxin production.No obvious differences in flagellum biosynthesis and motility were found between the tatABC mutant and the parental strain, showing a variable effect of Tat in different bacteria.

View Article: PubMed Central - HTML - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control, Department of Diarrheal Diseases, Chinese Center for Disease Control and Prevention, Beijing, PR China. zhanglijuan@icdc.cn

ABSTRACT

Background: The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to Vibrio cholerae has not been explored. Here we investigated the functionality of the Tat system in V. cholerae, the etiologic agent of cholera.

Results: In V. cholerae, the tatABC genes function in the translocation of TMAO reductase. Deletion of the tatABC genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in tatABC mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the tatABC mutant and the parental strain, showing a variable effect of Tat in different bacteria.

Conclusion: The Tat system contributes to the survival of V. cholerae in the environment and in vivo, and it may be associated with its virulence.

Show MeSH
Related in: MedlinePlus