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No detectable maternal effects of elevated CO(2) on Arabidopsis thaliana over 15 generations.

Teng N, Jin B, Wang Q, Hao H, Ceulemans R, Kuang T, Lin J - PLoS ONE (2009)

Bottom Line: We found that within an individual generation, elevated CO(2) significantly advanced plant flowering, increased photosynthetic rate, increased the size and number of starch grains per chloroplast, reduced stomatal density, stomatal conductance, and transpiration rate, and resulted in a higher reproductive mass.In addition, a reciprocal sowing experiment demonstrated that elevated CO(2) did not produce detectable maternal effects on the offspring after fifteen generations.Taken together, these results suggested that the maternal effects of elevated CO(2) failed to extend to the offspring due to the potential lack of genetic variation for CO(2) responsiveness, and future plants may not evolve specific adaptations to elevated CO(2) concentrations.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

ABSTRACT
Maternal environment has been demonstrated to produce considerable impact on offspring growth. However, few studies have been carried out to investigate multi-generational maternal effects of elevated CO(2) on plant growth and development. Here we present the first report on the responses of plant reproductive, photosynthetic, and cellular characteristics to elevated CO(2) over 15 generations using Arabidopsis thaliana as a model system. We found that within an individual generation, elevated CO(2) significantly advanced plant flowering, increased photosynthetic rate, increased the size and number of starch grains per chloroplast, reduced stomatal density, stomatal conductance, and transpiration rate, and resulted in a higher reproductive mass. Elevated CO(2) did not significantly influence silique length and number of seeds per silique. Across 15 generations grown at elevated CO(2) concentrations, however, there were no significant differences in these traits. In addition, a reciprocal sowing experiment demonstrated that elevated CO(2) did not produce detectable maternal effects on the offspring after fifteen generations. Taken together, these results suggested that the maternal effects of elevated CO(2) failed to extend to the offspring due to the potential lack of genetic variation for CO(2) responsiveness, and future plants may not evolve specific adaptations to elevated CO(2) concentrations.

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Days to first flower and number of siliques during the reciprocal sowing experiments.Open and solid bars indicate that seeds were obtained from the plants of the fifteenth generation grown at ambient and elevated CO2 concentrations, respectively. Seed source had no significant effect on days to first flower of plants (A) or number of siliques per plant (B).
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pone-0006035-g005: Days to first flower and number of siliques during the reciprocal sowing experiments.Open and solid bars indicate that seeds were obtained from the plants of the fifteenth generation grown at ambient and elevated CO2 concentrations, respectively. Seed source had no significant effect on days to first flower of plants (A) or number of siliques per plant (B).

Mentions: To evaluate whether Arabidopsis plants exhibited an adaptive response to elevated CO2, we conducted a reciprocal sowing experiment in which seeds from the fifteenth generation in each treatment were grown at both ambient and elevated CO2 concentrations. As a result, we did not detect significant interactions between the maternal CO2 environment and the CO2 transplant environment (Figure 5, and Tables 1, 2, 3). Plants from fifteenth-generation seeds grown under ambient and elevated CO2 were similar, with no significant differences in several traits between the two populations under either CO2 treatment regime. In other words, at a given CO2 concentration, the traits of both populations were similar to those observed at that CO2 level during the selection experiment. For example, the average time to first flowering was about 44 days in both populations when grown at ambient CO2 during the sowing experiment and was similar to that at ambient CO2 during the selection experiment (Figures 1A, 5A). Similarly, the average time to first flowering was about 40.5 days in both populations when plants were grown at elevated CO2, which was not significantly different from that at elevated CO2 during the selection experiment (Figures 1A, 5A). There were similar patterns for the change in silique number per plant, stomatal density in both adaxial and abaxial leaf surfaces, stomatal conductance, transpiration rate, photosynthetic rate, and chloroplast features during the sowing experiment (Figure 5, and Tables 1, 2, 3).


No detectable maternal effects of elevated CO(2) on Arabidopsis thaliana over 15 generations.

Teng N, Jin B, Wang Q, Hao H, Ceulemans R, Kuang T, Lin J - PLoS ONE (2009)

Days to first flower and number of siliques during the reciprocal sowing experiments.Open and solid bars indicate that seeds were obtained from the plants of the fifteenth generation grown at ambient and elevated CO2 concentrations, respectively. Seed source had no significant effect on days to first flower of plants (A) or number of siliques per plant (B).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2698214&req=5

pone-0006035-g005: Days to first flower and number of siliques during the reciprocal sowing experiments.Open and solid bars indicate that seeds were obtained from the plants of the fifteenth generation grown at ambient and elevated CO2 concentrations, respectively. Seed source had no significant effect on days to first flower of plants (A) or number of siliques per plant (B).
Mentions: To evaluate whether Arabidopsis plants exhibited an adaptive response to elevated CO2, we conducted a reciprocal sowing experiment in which seeds from the fifteenth generation in each treatment were grown at both ambient and elevated CO2 concentrations. As a result, we did not detect significant interactions between the maternal CO2 environment and the CO2 transplant environment (Figure 5, and Tables 1, 2, 3). Plants from fifteenth-generation seeds grown under ambient and elevated CO2 were similar, with no significant differences in several traits between the two populations under either CO2 treatment regime. In other words, at a given CO2 concentration, the traits of both populations were similar to those observed at that CO2 level during the selection experiment. For example, the average time to first flowering was about 44 days in both populations when grown at ambient CO2 during the sowing experiment and was similar to that at ambient CO2 during the selection experiment (Figures 1A, 5A). Similarly, the average time to first flowering was about 40.5 days in both populations when plants were grown at elevated CO2, which was not significantly different from that at elevated CO2 during the selection experiment (Figures 1A, 5A). There were similar patterns for the change in silique number per plant, stomatal density in both adaxial and abaxial leaf surfaces, stomatal conductance, transpiration rate, photosynthetic rate, and chloroplast features during the sowing experiment (Figure 5, and Tables 1, 2, 3).

Bottom Line: We found that within an individual generation, elevated CO(2) significantly advanced plant flowering, increased photosynthetic rate, increased the size and number of starch grains per chloroplast, reduced stomatal density, stomatal conductance, and transpiration rate, and resulted in a higher reproductive mass.In addition, a reciprocal sowing experiment demonstrated that elevated CO(2) did not produce detectable maternal effects on the offspring after fifteen generations.Taken together, these results suggested that the maternal effects of elevated CO(2) failed to extend to the offspring due to the potential lack of genetic variation for CO(2) responsiveness, and future plants may not evolve specific adaptations to elevated CO(2) concentrations.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

ABSTRACT
Maternal environment has been demonstrated to produce considerable impact on offspring growth. However, few studies have been carried out to investigate multi-generational maternal effects of elevated CO(2) on plant growth and development. Here we present the first report on the responses of plant reproductive, photosynthetic, and cellular characteristics to elevated CO(2) over 15 generations using Arabidopsis thaliana as a model system. We found that within an individual generation, elevated CO(2) significantly advanced plant flowering, increased photosynthetic rate, increased the size and number of starch grains per chloroplast, reduced stomatal density, stomatal conductance, and transpiration rate, and resulted in a higher reproductive mass. Elevated CO(2) did not significantly influence silique length and number of seeds per silique. Across 15 generations grown at elevated CO(2) concentrations, however, there were no significant differences in these traits. In addition, a reciprocal sowing experiment demonstrated that elevated CO(2) did not produce detectable maternal effects on the offspring after fifteen generations. Taken together, these results suggested that the maternal effects of elevated CO(2) failed to extend to the offspring due to the potential lack of genetic variation for CO(2) responsiveness, and future plants may not evolve specific adaptations to elevated CO(2) concentrations.

Show MeSH