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EC-18, a synthetic monoacetyldiacylglyceride, inhibits hematogenous metastasis of KIGB-5 biliary cancer cell in hamster model.

Kim MH, Chang HM, Kim TW, Lee SK, Park JS, Kim YH, Lee TY, Jang SJ, Suh CW, Lee TS, Kim SH, Lee SG - J. Korean Med. Sci. (2009)

Bottom Line: EC-18 (monoacetyldiacylglyceride) stimulates T cell production of IL-2, IL-4, IL-12, IFN-gamma, and GM-CSF in vitro.EC-18 treated groups showed only a few microscopic lung lesions and no evidence of metastatic lesion with highest dose whereas widespread gross lung lesions were observed in untreated control.In conclusion, EC-18 has a significant anti-tumor effect in this experimental model of biliary cancer suggesting potential for clinical application to this difficult cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.

ABSTRACT
EC-18 (monoacetyldiacylglyceride) stimulates T cell production of IL-2, IL-4, IL-12, IFN-gamma, and GM-CSF in vitro. To study the effects of these cytokines stimulated by EC-18 on cancer cells, we applied hamster biliary cancer model, a difficult cancer to treat. Cancer (KIGB-5) cells were given intravenously to produce hematogenous metastatic lung lesions which were treated with EC-18 at 10, 25, and 50 mg/kg/day respectively. The fourth group was untreated control. At 4th, 8th, and 12th week the lungs were examined. EC-18 treated groups showed only a few microscopic lung lesions and no evidence of metastatic lesion with highest dose whereas widespread gross lung lesions were observed in untreated control. To investigate whether the anti-tumor effect of EC-18 is associated with suppression of tumor cell Toll-like receptor 4 (TLR-4) expression in addition to stimulation of the immune cells, KIGB-5 cells were exposed to LPS with or without EC-18. TLR-4 mRNA and protein expression, measured by reverse transcriptase PCR (RT-PCR), real-time quantitative PCR and western blot analysis, showed suppression of TLR-4 expression in KIGB-5 cells treated with EC-18 compared with control. In conclusion, EC-18 has a significant anti-tumor effect in this experimental model of biliary cancer suggesting potential for clinical application to this difficult cancer.

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Related in: MedlinePlus

IL-2 secretion by 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) treated T-cells. Brown spots indicate IL-2 producing cells. Data are expressed as mean±S.E.M. *P<0.05 compared with the control group.
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Figure 1: IL-2 secretion by 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) treated T-cells. Brown spots indicate IL-2 producing cells. Data are expressed as mean±S.E.M. *P<0.05 compared with the control group.

Mentions: Following 5 days in culture with EC-18 1 µg/mL, the number of IL-2 producing cells was counted AID Elispot Reader System. The number of IL-2 producing T4 cells was increased 1.52-fold with EC-18 treatment which was statistically significant (P<0.05). The difference in the number of IL-2 producing T8 cells was not statistically significant (P<0.26) (Fig. 1).


EC-18, a synthetic monoacetyldiacylglyceride, inhibits hematogenous metastasis of KIGB-5 biliary cancer cell in hamster model.

Kim MH, Chang HM, Kim TW, Lee SK, Park JS, Kim YH, Lee TY, Jang SJ, Suh CW, Lee TS, Kim SH, Lee SG - J. Korean Med. Sci. (2009)

IL-2 secretion by 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) treated T-cells. Brown spots indicate IL-2 producing cells. Data are expressed as mean±S.E.M. *P<0.05 compared with the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2698195&req=5

Figure 1: IL-2 secretion by 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (EC-18) treated T-cells. Brown spots indicate IL-2 producing cells. Data are expressed as mean±S.E.M. *P<0.05 compared with the control group.
Mentions: Following 5 days in culture with EC-18 1 µg/mL, the number of IL-2 producing cells was counted AID Elispot Reader System. The number of IL-2 producing T4 cells was increased 1.52-fold with EC-18 treatment which was statistically significant (P<0.05). The difference in the number of IL-2 producing T8 cells was not statistically significant (P<0.26) (Fig. 1).

Bottom Line: EC-18 (monoacetyldiacylglyceride) stimulates T cell production of IL-2, IL-4, IL-12, IFN-gamma, and GM-CSF in vitro.EC-18 treated groups showed only a few microscopic lung lesions and no evidence of metastatic lesion with highest dose whereas widespread gross lung lesions were observed in untreated control.In conclusion, EC-18 has a significant anti-tumor effect in this experimental model of biliary cancer suggesting potential for clinical application to this difficult cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.

ABSTRACT
EC-18 (monoacetyldiacylglyceride) stimulates T cell production of IL-2, IL-4, IL-12, IFN-gamma, and GM-CSF in vitro. To study the effects of these cytokines stimulated by EC-18 on cancer cells, we applied hamster biliary cancer model, a difficult cancer to treat. Cancer (KIGB-5) cells were given intravenously to produce hematogenous metastatic lung lesions which were treated with EC-18 at 10, 25, and 50 mg/kg/day respectively. The fourth group was untreated control. At 4th, 8th, and 12th week the lungs were examined. EC-18 treated groups showed only a few microscopic lung lesions and no evidence of metastatic lesion with highest dose whereas widespread gross lung lesions were observed in untreated control. To investigate whether the anti-tumor effect of EC-18 is associated with suppression of tumor cell Toll-like receptor 4 (TLR-4) expression in addition to stimulation of the immune cells, KIGB-5 cells were exposed to LPS with or without EC-18. TLR-4 mRNA and protein expression, measured by reverse transcriptase PCR (RT-PCR), real-time quantitative PCR and western blot analysis, showed suppression of TLR-4 expression in KIGB-5 cells treated with EC-18 compared with control. In conclusion, EC-18 has a significant anti-tumor effect in this experimental model of biliary cancer suggesting potential for clinical application to this difficult cancer.

Show MeSH
Related in: MedlinePlus