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Hepatopulmonary syndrome induced by common bile duct ligation in a rabbit model: correlation between pulmonary vascular dilatation on thin-section CT and angiography and serum nitrite concentration or endothelial nitric oxide synthase (eNOS)1 expression.

Lee KN, Yoon SK, Lee JW, Kim KN, Park BH, Kwak JY, Jeong JS, Kim YH - Korean J Radiol (2004 Jul-Sep)

Bottom Line: Thin-section CT scans of the lung and pulmonary angiography were obtained 3 weeks after CBDL (n = 6), or a sham operation (n = 4), and intrapulmonary vasodilatation was assessed.The diameter and tortuosity of peripheral vessels in the right lower lobe by thin-section CT and angiography at the same level of the right lower lobe in all subjects were correlated to serum nitrite concentration and eNOS (endothelial nitric oxide synthase) expression as determined by immunostaining.Dilated pulmonary vessels were significantly correlated with an increased eNOS expression (r = 0.94, p < 0.0001), and the severity of pulmonary vessel tortuosity was found to be well correlated with serum nitrite concentration (r = 0.90, p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Diagnostic Radiology, Dong-A University College of Medicine, Seo-gu, Busan, Korea. kinamlee@chollian.net

ABSTRACT

Objective: To investigate the correlation between radiologic vascular dilatation and serum nitrite concentration and eNOS expression in the endothelial cell and pneumocyte in a rabbit model of hepatopulmonary syndrome induced by common bile duct ligation (CBDL).

Materials and methods: Thin-section CT scans of the lung and pulmonary angiography were obtained 3 weeks after CBDL (n = 6), or a sham operation (n = 4), and intrapulmonary vasodilatation was assessed. The diameter and tortuosity of peripheral vessels in the right lower lobe by thin-section CT and angiography at the same level of the right lower lobe in all subjects were correlated to serum nitrite concentration and eNOS (endothelial nitric oxide synthase) expression as determined by immunostaining.

Results: The diameters of pulmonary vessels on thin-section CT were well correlated with nitrite concentrations in serum (r = 0.92, p < 0.001). Dilated pulmonary vessels were significantly correlated with an increased eNOS expression (r = 0.94, p < 0.0001), and the severity of pulmonary vessel tortuosity was found to be well correlated with serum nitrite concentration (r = 0.90, p < 0.001).

Conclusion: The peripheral pulmonary vasculature in hepatopulmonary syndrome induced by CBLD was dilated on thin-section CT and on angiographs. Our findings suggest that peripheral pulmonary vascular dilatations are correlated with serum nitrite concentrations and pulmonary eNOS expression.

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Correlation between peripheral pulmonary vessel diameter and serum nitrite concentration in 6 animals 3 weeks after common bile duct ligation and in four animals in the control group. Peripheral pulmonary vessel diameter was found to correlate significantly with serum nitrite concentration (r = 0.92, p < 0.001).
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Figure 4: Correlation between peripheral pulmonary vessel diameter and serum nitrite concentration in 6 animals 3 weeks after common bile duct ligation and in four animals in the control group. Peripheral pulmonary vessel diameter was found to correlate significantly with serum nitrite concentration (r = 0.92, p < 0.001).

Mentions: In animals three weeks after CBDL we observed dilated and tortuous pulmonary vessels. Dilated vessels ranged from 1.3 to 2.2 mm in diameter (mean diameter ± standard deviation, 1.6 mm±0.3) in the right lower lobes (Figs. 1A, 2A); in contrast, the mean diameter of vessels in control animals that underwent a sham operation was 0.9 mm±0.1 (Fig. 3A). The mean diameters and standard errors of pulmonary vessel measurements in the right lower lobes of hepatopulmonary syndrome (HPS) by CT studies as determined by readers 1 and 2 were 1.6 mm±0.2 and 1.5 mm±0.5, respectively. Mean diameters and standard errors of vessel measurements in the right lower lobes of control animals by CT as determined readers 1 and 2 were 0.8 mm±0.2 and 0.9 mm±0.1, respectively. Interobserver agreement was thus moderate in terms of pulmonary vessel measurement in HPS and in controls, with weighted kappa coefficients of 0.58 (p < 0.05) and 0.52 (p < 0.05). We found that the diameters of pulmonary vessels by CT were well correlated with both nitrite concentration in serum (r = 0.92, p < 0.001) and with an elevated eNOS expression (r = 0.94, p < 0.0001) (Table 1, Figs. 1C, 2C, 3C, 4).


Hepatopulmonary syndrome induced by common bile duct ligation in a rabbit model: correlation between pulmonary vascular dilatation on thin-section CT and angiography and serum nitrite concentration or endothelial nitric oxide synthase (eNOS)1 expression.

Lee KN, Yoon SK, Lee JW, Kim KN, Park BH, Kwak JY, Jeong JS, Kim YH - Korean J Radiol (2004 Jul-Sep)

Correlation between peripheral pulmonary vessel diameter and serum nitrite concentration in 6 animals 3 weeks after common bile duct ligation and in four animals in the control group. Peripheral pulmonary vessel diameter was found to correlate significantly with serum nitrite concentration (r = 0.92, p < 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2698156&req=5

Figure 4: Correlation between peripheral pulmonary vessel diameter and serum nitrite concentration in 6 animals 3 weeks after common bile duct ligation and in four animals in the control group. Peripheral pulmonary vessel diameter was found to correlate significantly with serum nitrite concentration (r = 0.92, p < 0.001).
Mentions: In animals three weeks after CBDL we observed dilated and tortuous pulmonary vessels. Dilated vessels ranged from 1.3 to 2.2 mm in diameter (mean diameter ± standard deviation, 1.6 mm±0.3) in the right lower lobes (Figs. 1A, 2A); in contrast, the mean diameter of vessels in control animals that underwent a sham operation was 0.9 mm±0.1 (Fig. 3A). The mean diameters and standard errors of pulmonary vessel measurements in the right lower lobes of hepatopulmonary syndrome (HPS) by CT studies as determined by readers 1 and 2 were 1.6 mm±0.2 and 1.5 mm±0.5, respectively. Mean diameters and standard errors of vessel measurements in the right lower lobes of control animals by CT as determined readers 1 and 2 were 0.8 mm±0.2 and 0.9 mm±0.1, respectively. Interobserver agreement was thus moderate in terms of pulmonary vessel measurement in HPS and in controls, with weighted kappa coefficients of 0.58 (p < 0.05) and 0.52 (p < 0.05). We found that the diameters of pulmonary vessels by CT were well correlated with both nitrite concentration in serum (r = 0.92, p < 0.001) and with an elevated eNOS expression (r = 0.94, p < 0.0001) (Table 1, Figs. 1C, 2C, 3C, 4).

Bottom Line: Thin-section CT scans of the lung and pulmonary angiography were obtained 3 weeks after CBDL (n = 6), or a sham operation (n = 4), and intrapulmonary vasodilatation was assessed.The diameter and tortuosity of peripheral vessels in the right lower lobe by thin-section CT and angiography at the same level of the right lower lobe in all subjects were correlated to serum nitrite concentration and eNOS (endothelial nitric oxide synthase) expression as determined by immunostaining.Dilated pulmonary vessels were significantly correlated with an increased eNOS expression (r = 0.94, p < 0.0001), and the severity of pulmonary vessel tortuosity was found to be well correlated with serum nitrite concentration (r = 0.90, p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Diagnostic Radiology, Dong-A University College of Medicine, Seo-gu, Busan, Korea. kinamlee@chollian.net

ABSTRACT

Objective: To investigate the correlation between radiologic vascular dilatation and serum nitrite concentration and eNOS expression in the endothelial cell and pneumocyte in a rabbit model of hepatopulmonary syndrome induced by common bile duct ligation (CBDL).

Materials and methods: Thin-section CT scans of the lung and pulmonary angiography were obtained 3 weeks after CBDL (n = 6), or a sham operation (n = 4), and intrapulmonary vasodilatation was assessed. The diameter and tortuosity of peripheral vessels in the right lower lobe by thin-section CT and angiography at the same level of the right lower lobe in all subjects were correlated to serum nitrite concentration and eNOS (endothelial nitric oxide synthase) expression as determined by immunostaining.

Results: The diameters of pulmonary vessels on thin-section CT were well correlated with nitrite concentrations in serum (r = 0.92, p < 0.001). Dilated pulmonary vessels were significantly correlated with an increased eNOS expression (r = 0.94, p < 0.0001), and the severity of pulmonary vessel tortuosity was found to be well correlated with serum nitrite concentration (r = 0.90, p < 0.001).

Conclusion: The peripheral pulmonary vasculature in hepatopulmonary syndrome induced by CBLD was dilated on thin-section CT and on angiographs. Our findings suggest that peripheral pulmonary vascular dilatations are correlated with serum nitrite concentrations and pulmonary eNOS expression.

Show MeSH
Related in: MedlinePlus