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Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney.

Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ - Fungal Genet. Biol. (2008)

Bottom Line: Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge.To date few microarray studies have been performed on Candida albicans cells derived from infected tissues.When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.

ABSTRACT
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

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Comparison of the in vivo transcriptome (i.e. the subset of C. albicans SC5314 genes that were regulated during renal infections) with the in vivo phenome (i.e. the subset of C. albicans genes that affect the virulence of C. albicans, as defined by the Candida Genome Database @ August 2007) (Supplementary data).
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fig6: Comparison of the in vivo transcriptome (i.e. the subset of C. albicans SC5314 genes that were regulated during renal infections) with the in vivo phenome (i.e. the subset of C. albicans genes that affect the virulence of C. albicans, as defined by the Candida Genome Database @ August 2007) (Supplementary data).

Mentions: Our data suggest that changes in expression occur during infection, but that many of these changes may not be essential for infection (Section 3.3). To test this we examined the overlap between the subset of C. albicans genes whose expression was induced in vivo (Table 2) and the subset of C. albicans genes that are essential for virulence (i.e. those genes that have been annotated as having an impact upon virulence by the Candida Genome Database: www.candidagenome.org) (Fig. 6; Supplementary data). Four of the 148 C. albicans genes that have been shown to contribute to virulence were up-regulated in the rabbit kidney lesions. These were ALS1 and ALS2 (both GPI-anchored cell surface adhesins: Hoyer et al., 1995, 2001), CTA1 (which encodes catalase that contributes to oxidative stress protection: Wysong et al., 1998), and a gene of unknown function (orf19.1239). However, only a relatively small proportion of C. albicans genes have been virulence tested and the “phenome” of C. albicans is still very much incomplete. Indeed according to the Candida Genome database, only five of the C. albicans genes that were up-regulated in the rabbit kidney lesions have been virulence tested to date (Table 2). Four of these five genes are required for virulence.


Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney.

Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ - Fungal Genet. Biol. (2008)

Comparison of the in vivo transcriptome (i.e. the subset of C. albicans SC5314 genes that were regulated during renal infections) with the in vivo phenome (i.e. the subset of C. albicans genes that affect the virulence of C. albicans, as defined by the Candida Genome Database @ August 2007) (Supplementary data).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2698078&req=5

fig6: Comparison of the in vivo transcriptome (i.e. the subset of C. albicans SC5314 genes that were regulated during renal infections) with the in vivo phenome (i.e. the subset of C. albicans genes that affect the virulence of C. albicans, as defined by the Candida Genome Database @ August 2007) (Supplementary data).
Mentions: Our data suggest that changes in expression occur during infection, but that many of these changes may not be essential for infection (Section 3.3). To test this we examined the overlap between the subset of C. albicans genes whose expression was induced in vivo (Table 2) and the subset of C. albicans genes that are essential for virulence (i.e. those genes that have been annotated as having an impact upon virulence by the Candida Genome Database: www.candidagenome.org) (Fig. 6; Supplementary data). Four of the 148 C. albicans genes that have been shown to contribute to virulence were up-regulated in the rabbit kidney lesions. These were ALS1 and ALS2 (both GPI-anchored cell surface adhesins: Hoyer et al., 1995, 2001), CTA1 (which encodes catalase that contributes to oxidative stress protection: Wysong et al., 1998), and a gene of unknown function (orf19.1239). However, only a relatively small proportion of C. albicans genes have been virulence tested and the “phenome” of C. albicans is still very much incomplete. Indeed according to the Candida Genome database, only five of the C. albicans genes that were up-regulated in the rabbit kidney lesions have been virulence tested to date (Table 2). Four of these five genes are required for virulence.

Bottom Line: Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge.To date few microarray studies have been performed on Candida albicans cells derived from infected tissues.When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.

ABSTRACT
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

Show MeSH
Related in: MedlinePlus