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Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney.

Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ - Fungal Genet. Biol. (2008)

Bottom Line: Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge.To date few microarray studies have been performed on Candida albicans cells derived from infected tissues.When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.

ABSTRACT
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

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Comparison of genome-wide expression patterns for C. albicans NGY152 from different kidneys from the same animal versus kidneys from different animals. Mean correlation coefficients (±SD) for the pairwise comparisons of kidney infections using the whole microarray dataset for each kidney: **, significant at p < 0.01.
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fig5: Comparison of genome-wide expression patterns for C. albicans NGY152 from different kidneys from the same animal versus kidneys from different animals. Mean correlation coefficients (±SD) for the pairwise comparisons of kidney infections using the whole microarray dataset for each kidney: **, significant at p < 0.01.

Mentions: We compared the microarray data from individual kidneys infected with C. albicans NGY152. This was done by calculating pair-wise correlation coefficients for the global expression patterns for each kidney against all of the other kidneys. The mean correlation coefficient for the left and right kidneys from the same rabbit was significantly higher than for the mean correlation coefficient for kidneys from different rabbits (Fig. 5; Supplementary data). This indicates that the C. albicans expression profiles for cells infecting different kidneys in the same animal were more similar than the expression profiles from different animals (i.e. there is more biological variation between animals than between kidneys in the same animal). This is consistent with the idea that the behaviour of C. albicans is affected by the properties of the host and that variation between individual hosts can affect the expression profile of the pathogen. Our observation is also consistent with experimental variation in survival time that is generally observed for individual animals infected with equivalent inocula in mammalian models of disseminated candidiasis (MacCallum and Odds, 2005).


Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney.

Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ - Fungal Genet. Biol. (2008)

Comparison of genome-wide expression patterns for C. albicans NGY152 from different kidneys from the same animal versus kidneys from different animals. Mean correlation coefficients (±SD) for the pairwise comparisons of kidney infections using the whole microarray dataset for each kidney: **, significant at p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2698078&req=5

fig5: Comparison of genome-wide expression patterns for C. albicans NGY152 from different kidneys from the same animal versus kidneys from different animals. Mean correlation coefficients (±SD) for the pairwise comparisons of kidney infections using the whole microarray dataset for each kidney: **, significant at p < 0.01.
Mentions: We compared the microarray data from individual kidneys infected with C. albicans NGY152. This was done by calculating pair-wise correlation coefficients for the global expression patterns for each kidney against all of the other kidneys. The mean correlation coefficient for the left and right kidneys from the same rabbit was significantly higher than for the mean correlation coefficient for kidneys from different rabbits (Fig. 5; Supplementary data). This indicates that the C. albicans expression profiles for cells infecting different kidneys in the same animal were more similar than the expression profiles from different animals (i.e. there is more biological variation between animals than between kidneys in the same animal). This is consistent with the idea that the behaviour of C. albicans is affected by the properties of the host and that variation between individual hosts can affect the expression profile of the pathogen. Our observation is also consistent with experimental variation in survival time that is generally observed for individual animals infected with equivalent inocula in mammalian models of disseminated candidiasis (MacCallum and Odds, 2005).

Bottom Line: Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge.To date few microarray studies have been performed on Candida albicans cells derived from infected tissues.When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.

ABSTRACT
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

Show MeSH
Related in: MedlinePlus