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Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney.

Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ - Fungal Genet. Biol. (2008)

Bottom Line: Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge.To date few microarray studies have been performed on Candida albicans cells derived from infected tissues.When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.

ABSTRACT
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

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Comparison of qRT-PCR and microarray measurements of fold-regulation for six C. albicans SC5314 genes.
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fig1: Comparison of qRT-PCR and microarray measurements of fold-regulation for six C. albicans SC5314 genes.

Mentions: Relative to the RPMI 1640-grown control cells, 58 C. albicans genes were reproducibly induced by 2-fold or more in kidney lesions compared to the control cells in four independent replicate experiments (Table 2). These included genes involved in the assimilation of fatty acids and other alternative carbon sources (ACO1, ACS1, CIT1, FAA4, MLS1, POX4, SDH12), adhesion (ALS1, ALS2, ALS4), stress adaptation (CTA1, ENA22) and many genes of unknown function. In total, 50 genes were down-regulated in kidney lesions compared to control cells (Table 3). The down-regulated genes included functions associated with morphogenesis (ECE1, HYR1, RBT5), fermentation (CDC19, HGT11, HXK2, HXT5, HXT61, HXT62), protein biosynthesis (BEL1, RPL18, RPS13, RPS21) and genes associated with the cell surface (ALS10, HYR1, IHD1, PGA54, PGA59, PGA10, PHR1, RBT5, SUN41). To test the validity of these microarray datasets, we examined the expression levels of six genes by qRT-PCR. In all cases the qRT-PCR data displayed a high degree of concordance with the microarray data (Fig. 1).


Genome-wide analysis of Candida albicans gene expression patterns during infection of the mammalian kidney.

Walker LA, Maccallum DM, Bertram G, Gow NA, Odds FC, Brown AJ - Fungal Genet. Biol. (2008)

Comparison of qRT-PCR and microarray measurements of fold-regulation for six C. albicans SC5314 genes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2698078&req=5

fig1: Comparison of qRT-PCR and microarray measurements of fold-regulation for six C. albicans SC5314 genes.
Mentions: Relative to the RPMI 1640-grown control cells, 58 C. albicans genes were reproducibly induced by 2-fold or more in kidney lesions compared to the control cells in four independent replicate experiments (Table 2). These included genes involved in the assimilation of fatty acids and other alternative carbon sources (ACO1, ACS1, CIT1, FAA4, MLS1, POX4, SDH12), adhesion (ALS1, ALS2, ALS4), stress adaptation (CTA1, ENA22) and many genes of unknown function. In total, 50 genes were down-regulated in kidney lesions compared to control cells (Table 3). The down-regulated genes included functions associated with morphogenesis (ECE1, HYR1, RBT5), fermentation (CDC19, HGT11, HXK2, HXT5, HXT61, HXT62), protein biosynthesis (BEL1, RPL18, RPS13, RPS21) and genes associated with the cell surface (ALS10, HYR1, IHD1, PGA54, PGA59, PGA10, PHR1, RBT5, SUN41). To test the validity of these microarray datasets, we examined the expression levels of six genes by qRT-PCR. In all cases the qRT-PCR data displayed a high degree of concordance with the microarray data (Fig. 1).

Bottom Line: Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge.To date few microarray studies have been performed on Candida albicans cells derived from infected tissues.When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK.

ABSTRACT
Global analysis of the molecular responses of microbial pathogens to their mammalian hosts represents a major challenge. To date few microarray studies have been performed on Candida albicans cells derived from infected tissues. In this study we examined the C. albicans SC5314 transcriptome from renal infections in the rabbit. Genes involved in adhesion, stress adaptation and the assimilation of alternative carbon sources were up-regulated in these cells compared with control cells grown in RPMI 1640, whereas genes involved in morphogenesis, fermentation and translation were down-regulated. When we compared the congenic virulent C. albicans strains NGY152 and SC5314, there was minimal overlap between their transcriptomes during kidney infections. This suggests that much of the gene regulation observed during infections is not essential for virulence. Indeed, we observed a poor correlation between the transcriptome and phenome for those genes that were regulated during kidney infection and that have been virulence tested.

Show MeSH
Related in: MedlinePlus