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Neonatal pneumococcal conjugate vaccine immunization primes T cells for preferential Th2 cytokine expression: a randomized controlled trial in Papua New Guinea.

van den Biggelaar AH, Richmond PC, Pomat WS, Phuanukoonnon S, Nadal-Sims MA, Devitt CJ, Siba PM, Lehmann D, Holt PG - Vaccine (2009)

Bottom Line: Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only.T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls.Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

View Article: PubMed Central - PubMed

Affiliation: Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, PO Box 855, West Perth, WA 6872, Australia. Anitav@ichr.uwa.edu.au

ABSTRACT
The effects of neonatal immunization with 7-valent pneumococcal conjugate vaccine (7vPCV) on development of T-cell memory and general immune maturation were studied in a cohort of Papua New Guinean newborns. Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only. T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls. Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

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LPS-induced expression of inflammatory and cytotoxic mediators. PBMC of 3-month-old children in the neonatal 7vPCV (white bar), infant 7vPCV (grey bar) and control group (black bar) were stimulated in vitro with lipopolysaccharide (LPS). MRNA expression of IL-23, type-I interferon, Granzyme B and lymphotoxin-α were measured and normalized (ratio) for the expression of the housekeeping gene UBE2D2. Bars represent the geometric means and standard errors of geometric means of normalized mRNA expression in cells cultured in medium only (control) and LPS.
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fig4: LPS-induced expression of inflammatory and cytotoxic mediators. PBMC of 3-month-old children in the neonatal 7vPCV (white bar), infant 7vPCV (grey bar) and control group (black bar) were stimulated in vitro with lipopolysaccharide (LPS). MRNA expression of IL-23, type-I interferon, Granzyme B and lymphotoxin-α were measured and normalized (ratio) for the expression of the housekeeping gene UBE2D2. Bars represent the geometric means and standard errors of geometric means of normalized mRNA expression in cells cultured in medium only (control) and LPS.

Mentions: We further investigated the possible modulation of other inflammatory mediators including type-I interferon, IL-23, Granzyme B and lymphotoxin-α in response to LPS stimulation, but other than a reduced expression of IL-23 in the 7vPCV vaccination groups, no differences were found (Fig. 4). All three groups showed an enhanced release of mature IL-1β in response to LPS stimulation, but there were no significant differences between the groups (neonatal 315.5 ± 101.4 pg/ml, infant 334.1 ± 163.9 pg/ml, 303.6 ± 103.7 pg/ml) (p = 0.955).


Neonatal pneumococcal conjugate vaccine immunization primes T cells for preferential Th2 cytokine expression: a randomized controlled trial in Papua New Guinea.

van den Biggelaar AH, Richmond PC, Pomat WS, Phuanukoonnon S, Nadal-Sims MA, Devitt CJ, Siba PM, Lehmann D, Holt PG - Vaccine (2009)

LPS-induced expression of inflammatory and cytotoxic mediators. PBMC of 3-month-old children in the neonatal 7vPCV (white bar), infant 7vPCV (grey bar) and control group (black bar) were stimulated in vitro with lipopolysaccharide (LPS). MRNA expression of IL-23, type-I interferon, Granzyme B and lymphotoxin-α were measured and normalized (ratio) for the expression of the housekeeping gene UBE2D2. Bars represent the geometric means and standard errors of geometric means of normalized mRNA expression in cells cultured in medium only (control) and LPS.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2697326&req=5

fig4: LPS-induced expression of inflammatory and cytotoxic mediators. PBMC of 3-month-old children in the neonatal 7vPCV (white bar), infant 7vPCV (grey bar) and control group (black bar) were stimulated in vitro with lipopolysaccharide (LPS). MRNA expression of IL-23, type-I interferon, Granzyme B and lymphotoxin-α were measured and normalized (ratio) for the expression of the housekeeping gene UBE2D2. Bars represent the geometric means and standard errors of geometric means of normalized mRNA expression in cells cultured in medium only (control) and LPS.
Mentions: We further investigated the possible modulation of other inflammatory mediators including type-I interferon, IL-23, Granzyme B and lymphotoxin-α in response to LPS stimulation, but other than a reduced expression of IL-23 in the 7vPCV vaccination groups, no differences were found (Fig. 4). All three groups showed an enhanced release of mature IL-1β in response to LPS stimulation, but there were no significant differences between the groups (neonatal 315.5 ± 101.4 pg/ml, infant 334.1 ± 163.9 pg/ml, 303.6 ± 103.7 pg/ml) (p = 0.955).

Bottom Line: Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only.T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls.Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

View Article: PubMed Central - PubMed

Affiliation: Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, PO Box 855, West Perth, WA 6872, Australia. Anitav@ichr.uwa.edu.au

ABSTRACT
The effects of neonatal immunization with 7-valent pneumococcal conjugate vaccine (7vPCV) on development of T-cell memory and general immune maturation were studied in a cohort of Papua New Guinean newborns. Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only. T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls. Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

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