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Neonatal pneumococcal conjugate vaccine immunization primes T cells for preferential Th2 cytokine expression: a randomized controlled trial in Papua New Guinea.

van den Biggelaar AH, Richmond PC, Pomat WS, Phuanukoonnon S, Nadal-Sims MA, Devitt CJ, Siba PM, Lehmann D, Holt PG - Vaccine (2009)

Bottom Line: Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only.T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls.Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

View Article: PubMed Central - PubMed

Affiliation: Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, PO Box 855, West Perth, WA 6872, Australia. Anitav@ichr.uwa.edu.au

ABSTRACT
The effects of neonatal immunization with 7-valent pneumococcal conjugate vaccine (7vPCV) on development of T-cell memory and general immune maturation were studied in a cohort of Papua New Guinean newborns. Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only. T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls. Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

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Co-production of CRM197-specific Th1 and Th2 responses in neonatal, infant and control groups. Children were considered to produce Th1 responses to the 7vPCV protein carrier CRM197 when in vitro CRM197-induced IFN-γ responses were at least four times the background level, and Th2 responses if CRM197-induced IL-5 and/or IL-13 responses were at least four times the background. For each study group the proportion of children with no memory responses (hatched), Th2 without Th1 (light grey), mixed Th1/Th2 (dark grey) and Th1 without Th2 (black) is presented.
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fig2: Co-production of CRM197-specific Th1 and Th2 responses in neonatal, infant and control groups. Children were considered to produce Th1 responses to the 7vPCV protein carrier CRM197 when in vitro CRM197-induced IFN-γ responses were at least four times the background level, and Th2 responses if CRM197-induced IL-5 and/or IL-13 responses were at least four times the background. For each study group the proportion of children with no memory responses (hatched), Th2 without Th1 (light grey), mixed Th1/Th2 (dark grey) and Th1 without Th2 (black) is presented.

Mentions: As illustrated in Fig. 2, there was no difference in the proportion of Th1-CRM197 responders in the three groups, but in the neonatal group most of the Th1 responders (86%) produced Th2 cytokine responses (IL5-CRM197 and/or IL13-CRM197) in conjunction, whereas only 14% (6% of the total study group) produced Th1 responses ‘in isolation’ compared to 32% of the Th1 responders in the infant group (p = 0.072) (16% of total infant group) and 78% of the Th1 responders in the control group (34% of total control group) (p < 0.001).


Neonatal pneumococcal conjugate vaccine immunization primes T cells for preferential Th2 cytokine expression: a randomized controlled trial in Papua New Guinea.

van den Biggelaar AH, Richmond PC, Pomat WS, Phuanukoonnon S, Nadal-Sims MA, Devitt CJ, Siba PM, Lehmann D, Holt PG - Vaccine (2009)

Co-production of CRM197-specific Th1 and Th2 responses in neonatal, infant and control groups. Children were considered to produce Th1 responses to the 7vPCV protein carrier CRM197 when in vitro CRM197-induced IFN-γ responses were at least four times the background level, and Th2 responses if CRM197-induced IL-5 and/or IL-13 responses were at least four times the background. For each study group the proportion of children with no memory responses (hatched), Th2 without Th1 (light grey), mixed Th1/Th2 (dark grey) and Th1 without Th2 (black) is presented.
© Copyright Policy
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC2697326&req=5

fig2: Co-production of CRM197-specific Th1 and Th2 responses in neonatal, infant and control groups. Children were considered to produce Th1 responses to the 7vPCV protein carrier CRM197 when in vitro CRM197-induced IFN-γ responses were at least four times the background level, and Th2 responses if CRM197-induced IL-5 and/or IL-13 responses were at least four times the background. For each study group the proportion of children with no memory responses (hatched), Th2 without Th1 (light grey), mixed Th1/Th2 (dark grey) and Th1 without Th2 (black) is presented.
Mentions: As illustrated in Fig. 2, there was no difference in the proportion of Th1-CRM197 responders in the three groups, but in the neonatal group most of the Th1 responders (86%) produced Th2 cytokine responses (IL5-CRM197 and/or IL13-CRM197) in conjunction, whereas only 14% (6% of the total study group) produced Th1 responses ‘in isolation’ compared to 32% of the Th1 responders in the infant group (p = 0.072) (16% of total infant group) and 78% of the Th1 responders in the control group (34% of total control group) (p < 0.001).

Bottom Line: Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only.T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls.Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

View Article: PubMed Central - PubMed

Affiliation: Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, PO Box 855, West Perth, WA 6872, Australia. Anitav@ichr.uwa.edu.au

ABSTRACT
The effects of neonatal immunization with 7-valent pneumococcal conjugate vaccine (7vPCV) on development of T-cell memory and general immune maturation were studied in a cohort of Papua New Guinean newborns. Neonatal 7vPCV priming (followed by a dose at 1 and 2 months of age) was associated with enhanced Th2, but not Th1, cytokine responses to CRM(197) compared to 7vPCV at 1 and 2 months of age only. T cell responses to non-7vPCV vaccine antigens were similar in all groups, but TLR-mediated IL-6 and IL-10 responses were enhanced in 7vPCV vaccinated compared to controls. Neonatal 7vPCV vaccination primes T cell responses with a polarization towards Th2 with no bystander effects on other T cell responses.

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