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Dysregulation of heat shock protein 27 expression in oral tongue squamous cell carcinoma.

Wang A, Liu X, Sheng S, Ye H, Peng T, Shi F, Crowe DL, Zhou X - BMC Cancer (2009)

Bottom Line: Statistical analysis revealed that the reduced Hsp27 expression in primary tumor tissue was associated with poor differentiation.Furthermore, the higher expression of Hsp27 was correlated with better overall survival.Our study confirmed that the dysregulation of Hsp27 expression is a frequent event during the progression of OTSCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, USA. anxunwang@yahoo.com

ABSTRACT

Background: Recent proteomic studies identified Hsp27 as a highly over-expressed protein in oral squamous cell carcinoma (OSCC). Clinical studies that attempted to evaluate the prognostic values of Hsp27 yielded inconsistent results, which may be due to inclusion of OSCC cases from multiple anatomic sites. In this study, to determine the utility of Hsp27 for prognosis, we focused on oral tongue SCC (OTSCC), one of the most aggressive forms of OSCC.

Methods: Archival clinical samples of 15 normal oral tongue mucosa, 31 dysplastic lesions, 80 primary OTSCC, and 32 lymph node metastases were examined for Hsp27 expression by immunohistochemistry (IHC). Statistical analyses were carried out to assess the prognostic value of Hsp27 expression for patients with this disease.

Results: Dysregulation of Hsp27 expression was observed in dysplastic lesions, primary OTSCC, and lymph node metastases, and appears to be associated with disease progression. Statistical analysis revealed that the reduced Hsp27 expression in primary tumor tissue was associated with poor differentiation. Furthermore, the higher expression of Hsp27 was correlated with better overall survival.

Conclusion: Our study confirmed that the dysregulation of Hsp27 expression is a frequent event during the progression of OTSCC. The expression of Hsp27 appears to be an independent prognostic marker for patients with this disease.

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Related in: MedlinePlus

Immunohistochemistry analyses of Hsp27 expression on normal tongue, dysplastic lesions, primary oral tongue squamous cell carcinoma (OTSCC) and lymph node metastasis tissue samples. Immunohistochemistry analyses for Hsp27 were performed as described in Material and Methods on A: normal tongue mucosa (n = 15), B: dysplastic lesions (n = 31), C: well differentiated primary OTSCC (n = 46), D: moderately differentiated primary OTSCC (n = 20), E: poorly differentiated primary OTSCC (n = 14), and F: lymph node metastasis (n = 32). Representative Images (×200) were shown.
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Figure 1: Immunohistochemistry analyses of Hsp27 expression on normal tongue, dysplastic lesions, primary oral tongue squamous cell carcinoma (OTSCC) and lymph node metastasis tissue samples. Immunohistochemistry analyses for Hsp27 were performed as described in Material and Methods on A: normal tongue mucosa (n = 15), B: dysplastic lesions (n = 31), C: well differentiated primary OTSCC (n = 46), D: moderately differentiated primary OTSCC (n = 20), E: poorly differentiated primary OTSCC (n = 14), and F: lymph node metastasis (n = 32). Representative Images (×200) were shown.

Mentions: As illustrated in Figure 1, predominantly cytoplasmic staining for Hsp27 was observed in suprabasal keratinocytes in normal mucosa (Figure 1A). In general, the basal keratinocytes were not immunolabelled by anti-Hsp27 antibody. Among 15 normal mucosa that we examined, 4 cases (26.67%) showed no Hsp27 staining, 8 cases (53.33%) showed low staining, 2 cases (13.33%) showed moderate staining, and 1 case (6.67%) showed high staining. In dysplastic lesions (Figure 1B), the Hsp27 staining was observed in both suprabasal and basal keratinocytes. Of the 31 dysplastic lesions, 1 case (3.23%) showed no Hsp27 staining, 5 cases (16.13%) showed low staining, 11 cases (35.48%) showed moderate staining, and 14 cases (45.16%) showed high staining. In SCC (Figure 1C, D, E), diffuse Hsp27 staining was observed, where the high Hsp27 expression appears to be associated with differentiated areas. Of the 80 cases of primary OTSCC cases, 1 case (1.25%) showed no Hsp27 staining, 20 cases (25%) showed low staining, 42 cases (52.5%) showed moderate staining, and 17 cases (21.25%) showed high staining. For the 32 cases of lymph node metastatic disease (Figure 1F), low staining was observed in 8 cases (25%), moderate staining was observed in 12 cases (37.5%), and high staining was observed in 12 cases (37.5%). Thus, the dysregulation of Hsp27 consists of both the changes in cell population expressing this gene and the alterations in expression level. This change in Hsp27 expression pattern is consistent with our previous observations [22].


Dysregulation of heat shock protein 27 expression in oral tongue squamous cell carcinoma.

Wang A, Liu X, Sheng S, Ye H, Peng T, Shi F, Crowe DL, Zhou X - BMC Cancer (2009)

Immunohistochemistry analyses of Hsp27 expression on normal tongue, dysplastic lesions, primary oral tongue squamous cell carcinoma (OTSCC) and lymph node metastasis tissue samples. Immunohistochemistry analyses for Hsp27 were performed as described in Material and Methods on A: normal tongue mucosa (n = 15), B: dysplastic lesions (n = 31), C: well differentiated primary OTSCC (n = 46), D: moderately differentiated primary OTSCC (n = 20), E: poorly differentiated primary OTSCC (n = 14), and F: lymph node metastasis (n = 32). Representative Images (×200) were shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2696470&req=5

Figure 1: Immunohistochemistry analyses of Hsp27 expression on normal tongue, dysplastic lesions, primary oral tongue squamous cell carcinoma (OTSCC) and lymph node metastasis tissue samples. Immunohistochemistry analyses for Hsp27 were performed as described in Material and Methods on A: normal tongue mucosa (n = 15), B: dysplastic lesions (n = 31), C: well differentiated primary OTSCC (n = 46), D: moderately differentiated primary OTSCC (n = 20), E: poorly differentiated primary OTSCC (n = 14), and F: lymph node metastasis (n = 32). Representative Images (×200) were shown.
Mentions: As illustrated in Figure 1, predominantly cytoplasmic staining for Hsp27 was observed in suprabasal keratinocytes in normal mucosa (Figure 1A). In general, the basal keratinocytes were not immunolabelled by anti-Hsp27 antibody. Among 15 normal mucosa that we examined, 4 cases (26.67%) showed no Hsp27 staining, 8 cases (53.33%) showed low staining, 2 cases (13.33%) showed moderate staining, and 1 case (6.67%) showed high staining. In dysplastic lesions (Figure 1B), the Hsp27 staining was observed in both suprabasal and basal keratinocytes. Of the 31 dysplastic lesions, 1 case (3.23%) showed no Hsp27 staining, 5 cases (16.13%) showed low staining, 11 cases (35.48%) showed moderate staining, and 14 cases (45.16%) showed high staining. In SCC (Figure 1C, D, E), diffuse Hsp27 staining was observed, where the high Hsp27 expression appears to be associated with differentiated areas. Of the 80 cases of primary OTSCC cases, 1 case (1.25%) showed no Hsp27 staining, 20 cases (25%) showed low staining, 42 cases (52.5%) showed moderate staining, and 17 cases (21.25%) showed high staining. For the 32 cases of lymph node metastatic disease (Figure 1F), low staining was observed in 8 cases (25%), moderate staining was observed in 12 cases (37.5%), and high staining was observed in 12 cases (37.5%). Thus, the dysregulation of Hsp27 consists of both the changes in cell population expressing this gene and the alterations in expression level. This change in Hsp27 expression pattern is consistent with our previous observations [22].

Bottom Line: Statistical analysis revealed that the reduced Hsp27 expression in primary tumor tissue was associated with poor differentiation.Furthermore, the higher expression of Hsp27 was correlated with better overall survival.Our study confirmed that the dysregulation of Hsp27 expression is a frequent event during the progression of OTSCC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, USA. anxunwang@yahoo.com

ABSTRACT

Background: Recent proteomic studies identified Hsp27 as a highly over-expressed protein in oral squamous cell carcinoma (OSCC). Clinical studies that attempted to evaluate the prognostic values of Hsp27 yielded inconsistent results, which may be due to inclusion of OSCC cases from multiple anatomic sites. In this study, to determine the utility of Hsp27 for prognosis, we focused on oral tongue SCC (OTSCC), one of the most aggressive forms of OSCC.

Methods: Archival clinical samples of 15 normal oral tongue mucosa, 31 dysplastic lesions, 80 primary OTSCC, and 32 lymph node metastases were examined for Hsp27 expression by immunohistochemistry (IHC). Statistical analyses were carried out to assess the prognostic value of Hsp27 expression for patients with this disease.

Results: Dysregulation of Hsp27 expression was observed in dysplastic lesions, primary OTSCC, and lymph node metastases, and appears to be associated with disease progression. Statistical analysis revealed that the reduced Hsp27 expression in primary tumor tissue was associated with poor differentiation. Furthermore, the higher expression of Hsp27 was correlated with better overall survival.

Conclusion: Our study confirmed that the dysregulation of Hsp27 expression is a frequent event during the progression of OTSCC. The expression of Hsp27 appears to be an independent prognostic marker for patients with this disease.

Show MeSH
Related in: MedlinePlus