Limits...
Automated detection of residual cells after sex-mismatched stem-cell transplantation - evidence for presence of disease-marker negative residual cells.

Erlecke J, Hartmann I, Hoffmann M, Kroll T, Starke H, Heller A, Gloria A, Sayer HG, Johannes T, Claussen U, Liehr T, Loncarevic IF - Mol Cytogenet (2009)

Bottom Line: As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month).Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jena University Hospital, Institute of Human Genetics and Anthropology, Kollegiengasse 10, D-07743 Jena, Germany. i8lith@mti.uni-jena.de.

ABSTRACT

Background: A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.

Results: Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1-1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.

Conclusion: The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

No MeSH data available.


Related in: MedlinePlus

Sample plots for the conditional probability distribution  for the number of true acceptor cells MA in the sample for sample sizes M = 100 (A) and 500 (B). The prior is uninformative. The different curves correspond to different measured values M*A as indicated in the legends. These values correspond to 0%, 1%, 2% and 3% of the corresponding sample size. The distributions are biased to lower values of MA (see text).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2696465&req=5

Figure 3: Sample plots for the conditional probability distribution for the number of true acceptor cells MA in the sample for sample sizes M = 100 (A) and 500 (B). The prior is uninformative. The different curves correspond to different measured values M*A as indicated in the legends. These values correspond to 0%, 1%, 2% and 3% of the corresponding sample size. The distributions are biased to lower values of MA (see text).

Mentions: Sample plots of the conditional probability distribution are shown in Fig. 3 for the empirical prior. From these plots it becomes clear that the number of acceptor cells is rather overestimated by the measurements since the great majority of cells are donor cells that are occasionally classified as acceptor cells. The opposite case, i.e. acceptor cells being classified as donor cells, is very rare simply because there are only very few acceptor cells.


Automated detection of residual cells after sex-mismatched stem-cell transplantation - evidence for presence of disease-marker negative residual cells.

Erlecke J, Hartmann I, Hoffmann M, Kroll T, Starke H, Heller A, Gloria A, Sayer HG, Johannes T, Claussen U, Liehr T, Loncarevic IF - Mol Cytogenet (2009)

Sample plots for the conditional probability distribution  for the number of true acceptor cells MA in the sample for sample sizes M = 100 (A) and 500 (B). The prior is uninformative. The different curves correspond to different measured values M*A as indicated in the legends. These values correspond to 0%, 1%, 2% and 3% of the corresponding sample size. The distributions are biased to lower values of MA (see text).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2696465&req=5

Figure 3: Sample plots for the conditional probability distribution for the number of true acceptor cells MA in the sample for sample sizes M = 100 (A) and 500 (B). The prior is uninformative. The different curves correspond to different measured values M*A as indicated in the legends. These values correspond to 0%, 1%, 2% and 3% of the corresponding sample size. The distributions are biased to lower values of MA (see text).
Mentions: Sample plots of the conditional probability distribution are shown in Fig. 3 for the empirical prior. From these plots it becomes clear that the number of acceptor cells is rather overestimated by the measurements since the great majority of cells are donor cells that are occasionally classified as acceptor cells. The opposite case, i.e. acceptor cells being classified as donor cells, is very rare simply because there are only very few acceptor cells.

Bottom Line: As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month).Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jena University Hospital, Institute of Human Genetics and Anthropology, Kollegiengasse 10, D-07743 Jena, Germany. i8lith@mti.uni-jena.de.

ABSTRACT

Background: A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.

Results: Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1-1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.

Conclusion: The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

No MeSH data available.


Related in: MedlinePlus