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Automated detection of residual cells after sex-mismatched stem-cell transplantation - evidence for presence of disease-marker negative residual cells.

Erlecke J, Hartmann I, Hoffmann M, Kroll T, Starke H, Heller A, Gloria A, Sayer HG, Johannes T, Claussen U, Liehr T, Loncarevic IF - Mol Cytogenet (2009)

Bottom Line: As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month).Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jena University Hospital, Institute of Human Genetics and Anthropology, Kollegiengasse 10, D-07743 Jena, Germany. i8lith@mti.uni-jena.de.

ABSTRACT

Background: A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.

Results: Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1-1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.

Conclusion: The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

No MeSH data available.


Related in: MedlinePlus

Histogram for the misclassification error as estimated from the number of wrongly classified cells per sample. Blood samples were obtained from 10 healthy females and 11 healthy males. The histogram is fitted by a sum of two beta distributions.
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Figure 2: Histogram for the misclassification error as estimated from the number of wrongly classified cells per sample. Blood samples were obtained from 10 healthy females and 11 healthy males. The histogram is fitted by a sum of two beta distributions.

Mentions: Second statistical aspect, the error bounds for the fraction of acceptor cells in the blood sample were calculated with respect to classification errors introduced by the automated cell recognition device. The measured number of acceptor cells may not reflect the real number of acceptor cells in the sample due to measurement errors of the automated device. In order to assess the error rate of the device we classified 10 and 11 samples from healthy females and males, respectively, and obtained histograms for the fraction of misclassified cells. These were similar for females and males and thus pooled in a single distribution as shown in Fig. 2. Using this error distribution we calculated the probability density function for the true number of acceptor cells in the sample as described in the following paragraph. The resulting probability density function indicates that the measurements rather overestimate the number of true acceptor states.


Automated detection of residual cells after sex-mismatched stem-cell transplantation - evidence for presence of disease-marker negative residual cells.

Erlecke J, Hartmann I, Hoffmann M, Kroll T, Starke H, Heller A, Gloria A, Sayer HG, Johannes T, Claussen U, Liehr T, Loncarevic IF - Mol Cytogenet (2009)

Histogram for the misclassification error as estimated from the number of wrongly classified cells per sample. Blood samples were obtained from 10 healthy females and 11 healthy males. The histogram is fitted by a sum of two beta distributions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2696465&req=5

Figure 2: Histogram for the misclassification error as estimated from the number of wrongly classified cells per sample. Blood samples were obtained from 10 healthy females and 11 healthy males. The histogram is fitted by a sum of two beta distributions.
Mentions: Second statistical aspect, the error bounds for the fraction of acceptor cells in the blood sample were calculated with respect to classification errors introduced by the automated cell recognition device. The measured number of acceptor cells may not reflect the real number of acceptor cells in the sample due to measurement errors of the automated device. In order to assess the error rate of the device we classified 10 and 11 samples from healthy females and males, respectively, and obtained histograms for the fraction of misclassified cells. These were similar for females and males and thus pooled in a single distribution as shown in Fig. 2. Using this error distribution we calculated the probability density function for the true number of acceptor cells in the sample as described in the following paragraph. The resulting probability density function indicates that the measurements rather overestimate the number of true acceptor states.

Bottom Line: As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month).Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

View Article: PubMed Central - HTML - PubMed

Affiliation: Jena University Hospital, Institute of Human Genetics and Anthropology, Kollegiengasse 10, D-07743 Jena, Germany. i8lith@mti.uni-jena.de.

ABSTRACT

Background: A new chimerism analysis based on automated interphase fluorescence in situ hybridization (FISH) evaluation was established to detect residual cells after allogene sex-mismatched bone marrow or blood stem-cell transplantation.Cells of 58 patients were characterized as disease-associated due to presence of a bcr/abl-gene-fusion or a trisomy 8 and/or a simultaneous hybridization of gonosome-specific centromeric probes. The automatic slide scanning platform Metafer with its module MetaCyte was used to analyse 3,000 cells per sample.

Results: Overall 454 assays of 58 patients were analyzed. 13 of 58 patients showed residual recipient cells at one stage of more than 4% and 12 of 58 showed residual recipient cells less than 4%, respectively. As to be expected, patients of the latter group were associated with a higher survival rate (48 vs. 34 month). In only two of seven patients with disease-marker positive residual cells between 0.1-1.3% a relapse was observed. Besides, disease-marker negative residual cells were found in two patients without relapse at a rate of 2.8% and 3.3%, respectively.

Conclusion: The definite origin and meaning of disease-marker negative residual cells is still unclear. Overall, with the presented automatic chimerism analysis of interphase FISH slides, a sensitive method for detection of disease-marker positive residual cells is on hand.

No MeSH data available.


Related in: MedlinePlus