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Tissue expression of Squamous Cellular Carcinoma Antigen (SCCA) is inversely correlated to tumor size in HCC.

Trerotoli P, Fransvea E, Angelotti U, Antonaci G, Lupo L, Mazzocca A, Mangia A, Antonaci S, Giannelli G - Mol. Cancer (2009)

Bottom Line: All pairwise comparisons between groups yielded statistically significant differences.There was no statistically significant correlation between tissue and serum levels of SCCA.The significantly stronger expression of SCCA in smaller compared to larger HCC could be important for early HCC detection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Internal Medicine, Immunology and Infectious Diseases, Section of Internal Medicine, University of Bari Medical School, Bari, Italy. ptrerotoli@stamed.uniba.it

ABSTRACT

Background: This study aimed to investigate squamous cellular carcinoma antigen (SCCA) in serum and in tumoral and paired peritumoral tissues. We studied 27 patients with liver cirrhosis (LC) and 55 with HCC: 20 with a single nodule < 3 cm (s-HCC) and 35 with a single nodule > 3 cm or multifocal (l-HCC).

Methods: Serum SCCA was measured by the ELISA kit, and in frozen tissues by immunohistochemistry, quantified with appropriate imaging analysis software and expressed in square microns. Continuous variables are reported as means and 95% confidence intervals. Comparisons between independent groups were performed with a generalized linear model and Tukey grouping. Pearson's correlation coefficients were determined to evaluate relations between markers. Qualitative variables were summarized as count and percentage. Statistical significance was set at p-value < 0.05.

Results: Serum SCCA values in LC patients were 0.41 (0.31-0.55) ng/ml and statistically different from both HCC groups: 1.6 (1.0-2.6) ng/ml in s-HCC, 2.2 (1.28-2.74) ng/ml in l-HCC. SCCA in hepatic tissue was 263.8 (176.6-394.01) microm2 in LC patients, statistically different from values in s-HCC: 1163.2 (863.6-1566.8) microm2 and l-HCC: 625.8 (534.5-732.6). All pairwise comparisons between groups yielded statistically significant differences. Tumoral SCCA resulted linearly related with nodule size, showing a statistically significant inverse relation between the two variables (b = -0.099, p = 0.024).

Conclusion: There was no statistically significant correlation between tissue and serum levels of SCCA. The significantly stronger expression of SCCA in smaller compared to larger HCC could be important for early HCC detection. However, the increased expression in peritumoral tissue could affect the significance of serological detection.

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Related in: MedlinePlus

Tissue expression of SCCA. In Figure 1A, immunohistochemistry of SCCA in small and large HCC at low (A, C) and high (B, D) magnification. Paired peritumoral tissue at low magnification of small (E) and large (F) HCC. In A, C, E and F scale bar = 100 μm, in B and D scale bar = 50 μm. In Figure 1B, 95% Confidence intervals of neoplastic tissue SCCA in the different HCC patients groups.
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Figure 1: Tissue expression of SCCA. In Figure 1A, immunohistochemistry of SCCA in small and large HCC at low (A, C) and high (B, D) magnification. Paired peritumoral tissue at low magnification of small (E) and large (F) HCC. In A, C, E and F scale bar = 100 μm, in B and D scale bar = 50 μm. In Figure 1B, 95% Confidence intervals of neoplastic tissue SCCA in the different HCC patients groups.

Mentions: SCCA was detected by immunohistochemistry in all the patients, although with some differences, in both tumoral and peritumoral tissues of small and large HCC (Figure 1A). In particular, the mean (95% CI) expression in neoplastic tissue was 1163.2 (863.6–1566.8) μm2 in s-HCC and 625.8 (534.5–732.6) μm2 in l-HCC. There was a statistically significant difference among the groups (F = 17.45, p = 0.002) and Tukey grouping showed a significant difference (p < 0.05) between s-HCC vs l-HCC (Figure 1B). To further investigate the tissue expression of SCCA, paired peritumoral tissues were analyzed as well as LC samples used as proper control. The mean (95% CI) of SCCA tissue expression was 263.8 (176.6–394.01) μm2 in s-HCC peritumoral tissue, 345.49 (263,98 – 452,16) μm2 in l-HCC and 232.63 (163.3–331.38) μm2 in LC, Figure 2. The model did not result statistically significant (F = 1.84, p = 0.16). In conclusion, SCCA was more strongly expressed in the neoplastic tissue of smaller compared to larger HCC. In addition, it is noteworthy that the ratio between tumoral and peritumoral SCCA shows a trend ranging from 4.4 in s-HCC to 1.8 of l-HCC.


Tissue expression of Squamous Cellular Carcinoma Antigen (SCCA) is inversely correlated to tumor size in HCC.

Trerotoli P, Fransvea E, Angelotti U, Antonaci G, Lupo L, Mazzocca A, Mangia A, Antonaci S, Giannelli G - Mol. Cancer (2009)

Tissue expression of SCCA. In Figure 1A, immunohistochemistry of SCCA in small and large HCC at low (A, C) and high (B, D) magnification. Paired peritumoral tissue at low magnification of small (E) and large (F) HCC. In A, C, E and F scale bar = 100 μm, in B and D scale bar = 50 μm. In Figure 1B, 95% Confidence intervals of neoplastic tissue SCCA in the different HCC patients groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2693114&req=5

Figure 1: Tissue expression of SCCA. In Figure 1A, immunohistochemistry of SCCA in small and large HCC at low (A, C) and high (B, D) magnification. Paired peritumoral tissue at low magnification of small (E) and large (F) HCC. In A, C, E and F scale bar = 100 μm, in B and D scale bar = 50 μm. In Figure 1B, 95% Confidence intervals of neoplastic tissue SCCA in the different HCC patients groups.
Mentions: SCCA was detected by immunohistochemistry in all the patients, although with some differences, in both tumoral and peritumoral tissues of small and large HCC (Figure 1A). In particular, the mean (95% CI) expression in neoplastic tissue was 1163.2 (863.6–1566.8) μm2 in s-HCC and 625.8 (534.5–732.6) μm2 in l-HCC. There was a statistically significant difference among the groups (F = 17.45, p = 0.002) and Tukey grouping showed a significant difference (p < 0.05) between s-HCC vs l-HCC (Figure 1B). To further investigate the tissue expression of SCCA, paired peritumoral tissues were analyzed as well as LC samples used as proper control. The mean (95% CI) of SCCA tissue expression was 263.8 (176.6–394.01) μm2 in s-HCC peritumoral tissue, 345.49 (263,98 – 452,16) μm2 in l-HCC and 232.63 (163.3–331.38) μm2 in LC, Figure 2. The model did not result statistically significant (F = 1.84, p = 0.16). In conclusion, SCCA was more strongly expressed in the neoplastic tissue of smaller compared to larger HCC. In addition, it is noteworthy that the ratio between tumoral and peritumoral SCCA shows a trend ranging from 4.4 in s-HCC to 1.8 of l-HCC.

Bottom Line: All pairwise comparisons between groups yielded statistically significant differences.There was no statistically significant correlation between tissue and serum levels of SCCA.The significantly stronger expression of SCCA in smaller compared to larger HCC could be important for early HCC detection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Internal Medicine, Immunology and Infectious Diseases, Section of Internal Medicine, University of Bari Medical School, Bari, Italy. ptrerotoli@stamed.uniba.it

ABSTRACT

Background: This study aimed to investigate squamous cellular carcinoma antigen (SCCA) in serum and in tumoral and paired peritumoral tissues. We studied 27 patients with liver cirrhosis (LC) and 55 with HCC: 20 with a single nodule < 3 cm (s-HCC) and 35 with a single nodule > 3 cm or multifocal (l-HCC).

Methods: Serum SCCA was measured by the ELISA kit, and in frozen tissues by immunohistochemistry, quantified with appropriate imaging analysis software and expressed in square microns. Continuous variables are reported as means and 95% confidence intervals. Comparisons between independent groups were performed with a generalized linear model and Tukey grouping. Pearson's correlation coefficients were determined to evaluate relations between markers. Qualitative variables were summarized as count and percentage. Statistical significance was set at p-value < 0.05.

Results: Serum SCCA values in LC patients were 0.41 (0.31-0.55) ng/ml and statistically different from both HCC groups: 1.6 (1.0-2.6) ng/ml in s-HCC, 2.2 (1.28-2.74) ng/ml in l-HCC. SCCA in hepatic tissue was 263.8 (176.6-394.01) microm2 in LC patients, statistically different from values in s-HCC: 1163.2 (863.6-1566.8) microm2 and l-HCC: 625.8 (534.5-732.6). All pairwise comparisons between groups yielded statistically significant differences. Tumoral SCCA resulted linearly related with nodule size, showing a statistically significant inverse relation between the two variables (b = -0.099, p = 0.024).

Conclusion: There was no statistically significant correlation between tissue and serum levels of SCCA. The significantly stronger expression of SCCA in smaller compared to larger HCC could be important for early HCC detection. However, the increased expression in peritumoral tissue could affect the significance of serological detection.

Show MeSH
Related in: MedlinePlus