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The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance.

Ma QH - J. Exp. Bot. (2009)

Bottom Line: TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages.These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages.Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, Beijing 100093, China. mqh@ibcas.ac.cn

ABSTRACT
Stem lodging-resistance is an important phenotype in crop production. In the present study, the expression of the wheat COMT gene (TaCM) was determined in basal second internodes of lodging-resistant (H4564) and lodging-susceptible (C6001) cultivars at stem elongation, heading, and milky endosperm corresponding to Zadoks stages Z37, Z60, and Z75, respectively. The TaCM protein levels were analysed by protein gel blot and COMT enzyme activity was determined during the same stem developmental stages. TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages. The TaCM protein levels and COMT activity were also higher in H4564 than that in C6001 at the heading and milky stages. These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars. Southern analysis in a different population suggested that a TaCM locus was located in the distal region of chromosome 3BL, which has less investigated by QTL for lodging-resistant phenotype.

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Linkage maps of TaCM in the chromosome of wheat. CentiMorgan (cM) distances are indicated at the left of the chromosome and marker loci to the right. The map positions of TaCM are indicated in bold face.
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fig4: Linkage maps of TaCM in the chromosome of wheat. CentiMorgan (cM) distances are indicated at the left of the chromosome and marker loci to the right. The map positions of TaCM are indicated in bold face.

Mentions: TaCM protein levels in the basal second internodes of wheat stems were determined by protein gel blot analysis by using alfalfa monospecific polyclonal antisera against COMT (Fig. 3). A single band was detected from wheat stem samples; indicating that only one COMT protein isozyme was present in the stem tissue of wheat. This is in accordance with the genetic mapping results (Fig. 4), suggesting that TaCM exists as a single-copy gene in the wheat genome. The immunological reactivity of COMT protein from wheat stem extracts was similar to that of recombinant TaCM protein expressed in E. coli. This further supports a single COMT protein relating to the TaCM gene is present in stem tissues of wheat.


The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance.

Ma QH - J. Exp. Bot. (2009)

Linkage maps of TaCM in the chromosome of wheat. CentiMorgan (cM) distances are indicated at the left of the chromosome and marker loci to the right. The map positions of TaCM are indicated in bold face.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2692018&req=5

fig4: Linkage maps of TaCM in the chromosome of wheat. CentiMorgan (cM) distances are indicated at the left of the chromosome and marker loci to the right. The map positions of TaCM are indicated in bold face.
Mentions: TaCM protein levels in the basal second internodes of wheat stems were determined by protein gel blot analysis by using alfalfa monospecific polyclonal antisera against COMT (Fig. 3). A single band was detected from wheat stem samples; indicating that only one COMT protein isozyme was present in the stem tissue of wheat. This is in accordance with the genetic mapping results (Fig. 4), suggesting that TaCM exists as a single-copy gene in the wheat genome. The immunological reactivity of COMT protein from wheat stem extracts was similar to that of recombinant TaCM protein expressed in E. coli. This further supports a single COMT protein relating to the TaCM gene is present in stem tissues of wheat.

Bottom Line: TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages.These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages.Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, Beijing 100093, China. mqh@ibcas.ac.cn

ABSTRACT
Stem lodging-resistance is an important phenotype in crop production. In the present study, the expression of the wheat COMT gene (TaCM) was determined in basal second internodes of lodging-resistant (H4564) and lodging-susceptible (C6001) cultivars at stem elongation, heading, and milky endosperm corresponding to Zadoks stages Z37, Z60, and Z75, respectively. The TaCM protein levels were analysed by protein gel blot and COMT enzyme activity was determined during the same stem developmental stages. TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages. The TaCM protein levels and COMT activity were also higher in H4564 than that in C6001 at the heading and milky stages. These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars. Southern analysis in a different population suggested that a TaCM locus was located in the distal region of chromosome 3BL, which has less investigated by QTL for lodging-resistant phenotype.

Show MeSH