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The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance.

Ma QH - J. Exp. Bot. (2009)

Bottom Line: TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages.These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages.Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, Beijing 100093, China. mqh@ibcas.ac.cn

ABSTRACT
Stem lodging-resistance is an important phenotype in crop production. In the present study, the expression of the wheat COMT gene (TaCM) was determined in basal second internodes of lodging-resistant (H4564) and lodging-susceptible (C6001) cultivars at stem elongation, heading, and milky endosperm corresponding to Zadoks stages Z37, Z60, and Z75, respectively. The TaCM protein levels were analysed by protein gel blot and COMT enzyme activity was determined during the same stem developmental stages. TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages. The TaCM protein levels and COMT activity were also higher in H4564 than that in C6001 at the heading and milky stages. These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars. Southern analysis in a different population suggested that a TaCM locus was located in the distal region of chromosome 3BL, which has less investigated by QTL for lodging-resistant phenotype.

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RT-PCR analyses of TaCM gene expression in various wheat tissues. RT-PCR was performed using the gene-specific primers. Alpha-tubulin RT-PCR was included as a control.
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fig1: RT-PCR analyses of TaCM gene expression in various wheat tissues. RT-PCR was performed using the gene-specific primers. Alpha-tubulin RT-PCR was included as a control.

Mentions: RT-PCR analysis showed that the TaCM gene was constitutively expression in stem, leaf, and root tissues (Fig. 1), indicating that TaCM is associated with the constitutive lignification process in various vegetative growing tissues. TaCM gene expression was analysed in the basal second internodes at different stem developmental stages by Northern blot hybridization (Fig. 2). At the elongation stage, the TaCM mRNA levels in C6001 and H4564 were similar high. At the heading and milky stages, the TaCM mRNA levels in H4564 stem still remained very high, while these mRNA levels declined very markedly in C6001. Quantification of the TaCM1 mRNA levels with a Phosphor Image with 18S rRNA normalization confirmed these results. By contrast to the stem tissues, the TaCM mRNA levels in leaf tissues of the two wheat cultivars did not show much difference at different developmental stages (data not shown).


The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance.

Ma QH - J. Exp. Bot. (2009)

RT-PCR analyses of TaCM gene expression in various wheat tissues. RT-PCR was performed using the gene-specific primers. Alpha-tubulin RT-PCR was included as a control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2692018&req=5

fig1: RT-PCR analyses of TaCM gene expression in various wheat tissues. RT-PCR was performed using the gene-specific primers. Alpha-tubulin RT-PCR was included as a control.
Mentions: RT-PCR analysis showed that the TaCM gene was constitutively expression in stem, leaf, and root tissues (Fig. 1), indicating that TaCM is associated with the constitutive lignification process in various vegetative growing tissues. TaCM gene expression was analysed in the basal second internodes at different stem developmental stages by Northern blot hybridization (Fig. 2). At the elongation stage, the TaCM mRNA levels in C6001 and H4564 were similar high. At the heading and milky stages, the TaCM mRNA levels in H4564 stem still remained very high, while these mRNA levels declined very markedly in C6001. Quantification of the TaCM1 mRNA levels with a Phosphor Image with 18S rRNA normalization confirmed these results. By contrast to the stem tissues, the TaCM mRNA levels in leaf tissues of the two wheat cultivars did not show much difference at different developmental stages (data not shown).

Bottom Line: TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages.These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages.Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, Beijing 100093, China. mqh@ibcas.ac.cn

ABSTRACT
Stem lodging-resistance is an important phenotype in crop production. In the present study, the expression of the wheat COMT gene (TaCM) was determined in basal second internodes of lodging-resistant (H4564) and lodging-susceptible (C6001) cultivars at stem elongation, heading, and milky endosperm corresponding to Zadoks stages Z37, Z60, and Z75, respectively. The TaCM protein levels were analysed by protein gel blot and COMT enzyme activity was determined during the same stem developmental stages. TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages. The TaCM protein levels and COMT activity were also higher in H4564 than that in C6001 at the heading and milky stages. These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars. Southern analysis in a different population suggested that a TaCM locus was located in the distal region of chromosome 3BL, which has less investigated by QTL for lodging-resistant phenotype.

Show MeSH