Limits...
Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.

Li WF, Ding Q, Chen JJ, Cui KM, He XQ - J. Exp. Bot. (2009)

Bottom Line: Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns.The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation.The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

View Article: PubMed Central - PubMed

Affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.

ABSTRACT
Cell cycle progression requires interaction between cyclin-dependent kinase B (CDKB) and cyclin B (CYCB). The seasonal expression patterns of the CDKB and CYCB homologues from Populus tomentosa Carr. were investigated, and effects of temperature and exogenous indole-3-acetic acid (IAA) on their expression were further studied in water culture experiments. Based on the differential responses of dormant cambium cells to exogenous IAA, four stages of cambium dormancy were confirmed for P. tomentosa: quiescence 1 (Q1), rest, quiescence 2-1 (Q2-1), and quiescence 2-2 (Q2-2). PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases, weakly in Q1, and almost undetectable from rest until late Q2-2. Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns. Water culture experiments with temperature treatment further showed that a low temperature (4 degrees C) kept PtoCDKB and PtoCYCB transcripts at undetectable levels, while a warm temperature (25 degrees C) induced their expression in the cambium region. Meanwhile, water culture experiments with exogenous IAA treatment showed that induction of PtoCDKB and PtoCYCB transcription was independent of exogenous IAA. The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation. The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

Show MeSH

Related in: MedlinePlus

Seasonal expression patterns of PtoCDKB and PtoCYCB in the P. tomentosa cambium region and the climatic daily temperatures during the sampling period. (A) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR using UBO-L as the internal control in 2004 and 2005. (B) Climatic daily temperatures in 2004 and 2005. (C) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR in 2005 and 2006. (D) Climatic daily temperatures in 2005 and 2006. During the time between the left and right arrows, the PtoCDKB and PtoCYCB transcript levels were almost undetectable by RT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC2692011&req=5

fig4: Seasonal expression patterns of PtoCDKB and PtoCYCB in the P. tomentosa cambium region and the climatic daily temperatures during the sampling period. (A) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR using UBO-L as the internal control in 2004 and 2005. (B) Climatic daily temperatures in 2004 and 2005. (C) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR in 2005 and 2006. (D) Climatic daily temperatures in 2005 and 2006. During the time between the left and right arrows, the PtoCDKB and PtoCYCB transcript levels were almost undetectable by RT-PCR.

Mentions: The seasonal expression patterns of PtoCDKB and PtoCYCB in the cambium region were assayed by RT-PCR from March 2004 to March 2006. The PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases from May to August (Fig. 4A, C). The PtoCYCB transcripts were weakly expressed from September and undetectable after late November until early March of 2005 (Fig. 4A). Likewise, the PtoCDKB transcripts were weakly expressed from September and undetectable after the middle of November until early March of 2005 (Fig. 4A). Although a small difference existed in PtoCYCB expression, almost the same expression patterns of PtoCDKB and PtoCYCB were observed from 2005 to 2006 (Fig. 4C). The qRT-PCR analysis of PtoCDKB and PtoCYCB transcripts from May 2005 to March 2006 further confirmed these expression patterns (Fig. 5).


Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.

Li WF, Ding Q, Chen JJ, Cui KM, He XQ - J. Exp. Bot. (2009)

Seasonal expression patterns of PtoCDKB and PtoCYCB in the P. tomentosa cambium region and the climatic daily temperatures during the sampling period. (A) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR using UBO-L as the internal control in 2004 and 2005. (B) Climatic daily temperatures in 2004 and 2005. (C) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR in 2005 and 2006. (D) Climatic daily temperatures in 2005 and 2006. During the time between the left and right arrows, the PtoCDKB and PtoCYCB transcript levels were almost undetectable by RT-PCR.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2692011&req=5

fig4: Seasonal expression patterns of PtoCDKB and PtoCYCB in the P. tomentosa cambium region and the climatic daily temperatures during the sampling period. (A) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR using UBO-L as the internal control in 2004 and 2005. (B) Climatic daily temperatures in 2004 and 2005. (C) Periodic expression patterns of PtoCDKB and PtoCYCB investigated by RT-PCR in 2005 and 2006. (D) Climatic daily temperatures in 2005 and 2006. During the time between the left and right arrows, the PtoCDKB and PtoCYCB transcript levels were almost undetectable by RT-PCR.
Mentions: The seasonal expression patterns of PtoCDKB and PtoCYCB in the cambium region were assayed by RT-PCR from March 2004 to March 2006. The PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases from May to August (Fig. 4A, C). The PtoCYCB transcripts were weakly expressed from September and undetectable after late November until early March of 2005 (Fig. 4A). Likewise, the PtoCDKB transcripts were weakly expressed from September and undetectable after the middle of November until early March of 2005 (Fig. 4A). Although a small difference existed in PtoCYCB expression, almost the same expression patterns of PtoCDKB and PtoCYCB were observed from 2005 to 2006 (Fig. 4C). The qRT-PCR analysis of PtoCDKB and PtoCYCB transcripts from May 2005 to March 2006 further confirmed these expression patterns (Fig. 5).

Bottom Line: Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns.The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation.The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

View Article: PubMed Central - PubMed

Affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.

ABSTRACT
Cell cycle progression requires interaction between cyclin-dependent kinase B (CDKB) and cyclin B (CYCB). The seasonal expression patterns of the CDKB and CYCB homologues from Populus tomentosa Carr. were investigated, and effects of temperature and exogenous indole-3-acetic acid (IAA) on their expression were further studied in water culture experiments. Based on the differential responses of dormant cambium cells to exogenous IAA, four stages of cambium dormancy were confirmed for P. tomentosa: quiescence 1 (Q1), rest, quiescence 2-1 (Q2-1), and quiescence 2-2 (Q2-2). PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases, weakly in Q1, and almost undetectable from rest until late Q2-2. Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns. Water culture experiments with temperature treatment further showed that a low temperature (4 degrees C) kept PtoCDKB and PtoCYCB transcripts at undetectable levels, while a warm temperature (25 degrees C) induced their expression in the cambium region. Meanwhile, water culture experiments with exogenous IAA treatment showed that induction of PtoCDKB and PtoCYCB transcription was independent of exogenous IAA. The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation. The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

Show MeSH
Related in: MedlinePlus