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Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.

Li WF, Ding Q, Chen JJ, Cui KM, He XQ - J. Exp. Bot. (2009)

Bottom Line: Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns.The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation.The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

View Article: PubMed Central - PubMed

Affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.

ABSTRACT
Cell cycle progression requires interaction between cyclin-dependent kinase B (CDKB) and cyclin B (CYCB). The seasonal expression patterns of the CDKB and CYCB homologues from Populus tomentosa Carr. were investigated, and effects of temperature and exogenous indole-3-acetic acid (IAA) on their expression were further studied in water culture experiments. Based on the differential responses of dormant cambium cells to exogenous IAA, four stages of cambium dormancy were confirmed for P. tomentosa: quiescence 1 (Q1), rest, quiescence 2-1 (Q2-1), and quiescence 2-2 (Q2-2). PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases, weakly in Q1, and almost undetectable from rest until late Q2-2. Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns. Water culture experiments with temperature treatment further showed that a low temperature (4 degrees C) kept PtoCDKB and PtoCYCB transcripts at undetectable levels, while a warm temperature (25 degrees C) induced their expression in the cambium region. Meanwhile, water culture experiments with exogenous IAA treatment showed that induction of PtoCDKB and PtoCYCB transcription was independent of exogenous IAA. The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation. The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

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CLUSTALX (1.81) multiple sequence alignment of CDKB2; 2 and CYCB2; 4 amino acid sequences. Family-specific protein signatures are shaded grey. Identical residues are indicated by asterisks. (A) Multiple sequence alignment of CDKB2; 2. The GenBank accession no. for AtCDKB2; 2 is NP_173517.1. (B) Multiple sequence alignment of CYCB2; 4.
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fig3: CLUSTALX (1.81) multiple sequence alignment of CDKB2; 2 and CYCB2; 4 amino acid sequences. Family-specific protein signatures are shaded grey. Identical residues are indicated by asterisks. (A) Multiple sequence alignment of CDKB2; 2. The GenBank accession no. for AtCDKB2; 2 is NP_173517.1. (B) Multiple sequence alignment of CYCB2; 4.

Mentions: The full-length cDNAs for the CDKB and CYCB homologues from P. tomentosa, PtoCDKB and PtoCYCB, were cloned. The deduced amino acid sequence for PtoCDKB displayed high homology to PttCDKB from P. tremula×P. tremuloides and PtCDKB from P. trichocarpa, with sequence identity of up to 99% (303/306 amino acids) (Fig. 3A). The sequence was also 306 amino acids long and contained a PPTTLRE motif characteristic of the CDKB2; 2 protein type (Vandepoele et al., 2002) (Fig. 3A).


Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.

Li WF, Ding Q, Chen JJ, Cui KM, He XQ - J. Exp. Bot. (2009)

CLUSTALX (1.81) multiple sequence alignment of CDKB2; 2 and CYCB2; 4 amino acid sequences. Family-specific protein signatures are shaded grey. Identical residues are indicated by asterisks. (A) Multiple sequence alignment of CDKB2; 2. The GenBank accession no. for AtCDKB2; 2 is NP_173517.1. (B) Multiple sequence alignment of CYCB2; 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC2692011&req=5

fig3: CLUSTALX (1.81) multiple sequence alignment of CDKB2; 2 and CYCB2; 4 amino acid sequences. Family-specific protein signatures are shaded grey. Identical residues are indicated by asterisks. (A) Multiple sequence alignment of CDKB2; 2. The GenBank accession no. for AtCDKB2; 2 is NP_173517.1. (B) Multiple sequence alignment of CYCB2; 4.
Mentions: The full-length cDNAs for the CDKB and CYCB homologues from P. tomentosa, PtoCDKB and PtoCYCB, were cloned. The deduced amino acid sequence for PtoCDKB displayed high homology to PttCDKB from P. tremula×P. tremuloides and PtCDKB from P. trichocarpa, with sequence identity of up to 99% (303/306 amino acids) (Fig. 3A). The sequence was also 306 amino acids long and contained a PPTTLRE motif characteristic of the CDKB2; 2 protein type (Vandepoele et al., 2002) (Fig. 3A).

Bottom Line: Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns.The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation.The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

View Article: PubMed Central - PubMed

Affiliation: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China.

ABSTRACT
Cell cycle progression requires interaction between cyclin-dependent kinase B (CDKB) and cyclin B (CYCB). The seasonal expression patterns of the CDKB and CYCB homologues from Populus tomentosa Carr. were investigated, and effects of temperature and exogenous indole-3-acetic acid (IAA) on their expression were further studied in water culture experiments. Based on the differential responses of dormant cambium cells to exogenous IAA, four stages of cambium dormancy were confirmed for P. tomentosa: quiescence 1 (Q1), rest, quiescence 2-1 (Q2-1), and quiescence 2-2 (Q2-2). PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases, weakly in Q1, and almost undetectable from rest until late Q2-2. Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns. Water culture experiments with temperature treatment further showed that a low temperature (4 degrees C) kept PtoCDKB and PtoCYCB transcripts at undetectable levels, while a warm temperature (25 degrees C) induced their expression in the cambium region. Meanwhile, water culture experiments with exogenous IAA treatment showed that induction of PtoCDKB and PtoCYCB transcription was independent of exogenous IAA. The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation. The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division.

Show MeSH
Related in: MedlinePlus