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Exercise-training in young Drosophila melanogaster reduces age-related decline in mobility and cardiac performance.

Piazza N, Gosangi B, Devilla S, Arking R, Wessells R - PLoS ONE (2009)

Bottom Line: Exercise-based therapies have shown great promise in sustaining mobility in elderly patients, as well as in rodent models.When young flies are subjected to a carefully controlled, ramped paradigm of exercise-training, they display significant reduction in age-related decline in mobility and cardiac performance.Fly lines with improved mitochondrial efficiency display some of the phenotypes observed in wild-type exercised flies.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Institute of Gerontology, University of Michigan Medical School, Ann Arbor, MI, USA.

ABSTRACT
Declining mobility is a major concern, as well as a major source of health care costs, among the elderly population. Lack of mobility is a primary cause of entry into managed care facilities, and a contributing factor to the frequency of damaging falls. Exercise-based therapies have shown great promise in sustaining mobility in elderly patients, as well as in rodent models. However, the genetic basis of the changing physiological responses to exercise during aging is not well understood. Here, we describe the first exercise-training paradigm in an invertebrate genetic model system. Flies are exercised by a mechanized platform, known as the Power Tower, that rapidly, repeatedly, induces their innate instinct for negative geotaxis. When young flies are subjected to a carefully controlled, ramped paradigm of exercise-training, they display significant reduction in age-related decline in mobility and cardiac performance. Fly lines with improved mitochondrial efficiency display some of the phenotypes observed in wild-type exercised flies. The exercise response in flies is influenced by the amount of protein and lipid, but not carbohydrate, in the diet. The development of an exercise-training model in Drosophila melanogaster opens the way to direct testing of single-gene based genetic therapies for improved mobility in aged animals, as well as unbiased genetic screens for loci involved in the changing response to exercise during aging.

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Cardiac stress resistance tests for exercise-trained and unexercised flies.(A): There is no significant difference in fibrillation rate of y1w67c23 flies after cardiac electrical pacing between exercised (blue diamonds) and unexercised flies (red squares) (multivariate regression, treatment-by-age: p = 0.1947, chi-square = 1.682). (B): y1w67c23 cardiac arrest rate in response to electrical pacing is shown for exercise and unexercised flies across five weeks. Exercise-trained flies (blue diamonds) display a lower arrest rate than unexercised flies (red squares) by five weeks (t-test, p = 0.001). (C): y1w67c23 exercise-trained flies (blue diamonds) have a higher arrest-recovery rate at five weeks than unexercised flies (red squares) (t-test, p = 0.008). All lines represent straight connective lines between data points, and are not fitted to a best-fit model.
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pone-0005886-g003: Cardiac stress resistance tests for exercise-trained and unexercised flies.(A): There is no significant difference in fibrillation rate of y1w67c23 flies after cardiac electrical pacing between exercised (blue diamonds) and unexercised flies (red squares) (multivariate regression, treatment-by-age: p = 0.1947, chi-square = 1.682). (B): y1w67c23 cardiac arrest rate in response to electrical pacing is shown for exercise and unexercised flies across five weeks. Exercise-trained flies (blue diamonds) display a lower arrest rate than unexercised flies (red squares) by five weeks (t-test, p = 0.001). (C): y1w67c23 exercise-trained flies (blue diamonds) have a higher arrest-recovery rate at five weeks than unexercised flies (red squares) (t-test, p = 0.008). All lines represent straight connective lines between data points, and are not fitted to a best-fit model.

Mentions: y1w67c23 flies showed a significant difference in fibrillation rate in response to pacing (Fig. 3A), but showed a statistically significant improvement in rate of pacing-induced arrest at advanced ages (t-test at five weeks, y1w67c23, p = .001, Fig. 3B). Furthermore, among those that experienced arrest, a significantly higher percentage was able to recover a normal heartbeat within two minutes (t-test at five weeks, y1w67c23, p = .008, Fig. 3C).


Exercise-training in young Drosophila melanogaster reduces age-related decline in mobility and cardiac performance.

Piazza N, Gosangi B, Devilla S, Arking R, Wessells R - PLoS ONE (2009)

Cardiac stress resistance tests for exercise-trained and unexercised flies.(A): There is no significant difference in fibrillation rate of y1w67c23 flies after cardiac electrical pacing between exercised (blue diamonds) and unexercised flies (red squares) (multivariate regression, treatment-by-age: p = 0.1947, chi-square = 1.682). (B): y1w67c23 cardiac arrest rate in response to electrical pacing is shown for exercise and unexercised flies across five weeks. Exercise-trained flies (blue diamonds) display a lower arrest rate than unexercised flies (red squares) by five weeks (t-test, p = 0.001). (C): y1w67c23 exercise-trained flies (blue diamonds) have a higher arrest-recovery rate at five weeks than unexercised flies (red squares) (t-test, p = 0.008). All lines represent straight connective lines between data points, and are not fitted to a best-fit model.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2691613&req=5

pone-0005886-g003: Cardiac stress resistance tests for exercise-trained and unexercised flies.(A): There is no significant difference in fibrillation rate of y1w67c23 flies after cardiac electrical pacing between exercised (blue diamonds) and unexercised flies (red squares) (multivariate regression, treatment-by-age: p = 0.1947, chi-square = 1.682). (B): y1w67c23 cardiac arrest rate in response to electrical pacing is shown for exercise and unexercised flies across five weeks. Exercise-trained flies (blue diamonds) display a lower arrest rate than unexercised flies (red squares) by five weeks (t-test, p = 0.001). (C): y1w67c23 exercise-trained flies (blue diamonds) have a higher arrest-recovery rate at five weeks than unexercised flies (red squares) (t-test, p = 0.008). All lines represent straight connective lines between data points, and are not fitted to a best-fit model.
Mentions: y1w67c23 flies showed a significant difference in fibrillation rate in response to pacing (Fig. 3A), but showed a statistically significant improvement in rate of pacing-induced arrest at advanced ages (t-test at five weeks, y1w67c23, p = .001, Fig. 3B). Furthermore, among those that experienced arrest, a significantly higher percentage was able to recover a normal heartbeat within two minutes (t-test at five weeks, y1w67c23, p = .008, Fig. 3C).

Bottom Line: Exercise-based therapies have shown great promise in sustaining mobility in elderly patients, as well as in rodent models.When young flies are subjected to a carefully controlled, ramped paradigm of exercise-training, they display significant reduction in age-related decline in mobility and cardiac performance.Fly lines with improved mitochondrial efficiency display some of the phenotypes observed in wild-type exercised flies.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Institute of Gerontology, University of Michigan Medical School, Ann Arbor, MI, USA.

ABSTRACT
Declining mobility is a major concern, as well as a major source of health care costs, among the elderly population. Lack of mobility is a primary cause of entry into managed care facilities, and a contributing factor to the frequency of damaging falls. Exercise-based therapies have shown great promise in sustaining mobility in elderly patients, as well as in rodent models. However, the genetic basis of the changing physiological responses to exercise during aging is not well understood. Here, we describe the first exercise-training paradigm in an invertebrate genetic model system. Flies are exercised by a mechanized platform, known as the Power Tower, that rapidly, repeatedly, induces their innate instinct for negative geotaxis. When young flies are subjected to a carefully controlled, ramped paradigm of exercise-training, they display significant reduction in age-related decline in mobility and cardiac performance. Fly lines with improved mitochondrial efficiency display some of the phenotypes observed in wild-type exercised flies. The exercise response in flies is influenced by the amount of protein and lipid, but not carbohydrate, in the diet. The development of an exercise-training model in Drosophila melanogaster opens the way to direct testing of single-gene based genetic therapies for improved mobility in aged animals, as well as unbiased genetic screens for loci involved in the changing response to exercise during aging.

Show MeSH
Related in: MedlinePlus