Limits...
Infectious prions in pre-clinical deer and transmission of chronic wasting disease solely by environmental exposure.

Mathiason CK, Hays SA, Powers J, Hayes-Klug J, Langenberg J, Dahmes SJ, Osborn DA, Miller KV, Warren RJ, Mason GL, Hoover EA - PLoS ONE (2009)

Bottom Line: Key to understanding the epidemiology and pathogenesis of prion diseases, including chronic wasting disease (CWD) of cervids, is determining the mode of transmission from one individual to another.Here we again use bioassays in deer to show that blood and saliva of pre-symptomatic deer contain infectious prions capable of infecting naïve deer and that naïve deer exposed only to environmental fomites from the suites of CWD-infected deer acquired CWD infection after a period of 15 months post initial exposure.These results help to further explain the basis for the facile transmission of CWD, highlight the complexities associated with CWD transmission among cervids in their natural environment, emphasize the potential utility of blood-based testing to detect pre-clinical CWD infection, and could augur similar transmission dynamics in other prion infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT
Key to understanding the epidemiology and pathogenesis of prion diseases, including chronic wasting disease (CWD) of cervids, is determining the mode of transmission from one individual to another. We have previously reported that saliva and blood from CWD-infected deer contain sufficient infectious prions to transmit disease upon passage into naïve deer. Here we again use bioassays in deer to show that blood and saliva of pre-symptomatic deer contain infectious prions capable of infecting naïve deer and that naïve deer exposed only to environmental fomites from the suites of CWD-infected deer acquired CWD infection after a period of 15 months post initial exposure. These results help to further explain the basis for the facile transmission of CWD, highlight the complexities associated with CWD transmission among cervids in their natural environment, emphasize the potential utility of blood-based testing to detect pre-clinical CWD infection, and could augur similar transmission dynamics in other prion infections.

Show MeSH

Related in: MedlinePlus

PrPCWD detection by longitudinal tonsil biopsy and terminal necropsy.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2691594&req=5

pone-0005916-g001: PrPCWD detection by longitudinal tonsil biopsy and terminal necropsy.

Mentions: Each naïve deer received blood by the IV route from pre-clinical CWD+ source deer that were 10–12 months post inoculation (pi) (Tables 1, 2). Two of three recipient deer became PrPCWD tonsil biopsy positive at 12 months pi, but not at earlier sampling intervals (Fig. 1). At 19 months pi, when the cohort was necropsied, all three deer were CWD+, as indicated by detection of PrPCWD in the medulla oblongata at the level of the obex (medulla at obex) and in lymphoid tissue (Fig. 2).


Infectious prions in pre-clinical deer and transmission of chronic wasting disease solely by environmental exposure.

Mathiason CK, Hays SA, Powers J, Hayes-Klug J, Langenberg J, Dahmes SJ, Osborn DA, Miller KV, Warren RJ, Mason GL, Hoover EA - PLoS ONE (2009)

PrPCWD detection by longitudinal tonsil biopsy and terminal necropsy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2691594&req=5

pone-0005916-g001: PrPCWD detection by longitudinal tonsil biopsy and terminal necropsy.
Mentions: Each naïve deer received blood by the IV route from pre-clinical CWD+ source deer that were 10–12 months post inoculation (pi) (Tables 1, 2). Two of three recipient deer became PrPCWD tonsil biopsy positive at 12 months pi, but not at earlier sampling intervals (Fig. 1). At 19 months pi, when the cohort was necropsied, all three deer were CWD+, as indicated by detection of PrPCWD in the medulla oblongata at the level of the obex (medulla at obex) and in lymphoid tissue (Fig. 2).

Bottom Line: Key to understanding the epidemiology and pathogenesis of prion diseases, including chronic wasting disease (CWD) of cervids, is determining the mode of transmission from one individual to another.Here we again use bioassays in deer to show that blood and saliva of pre-symptomatic deer contain infectious prions capable of infecting naïve deer and that naïve deer exposed only to environmental fomites from the suites of CWD-infected deer acquired CWD infection after a period of 15 months post initial exposure.These results help to further explain the basis for the facile transmission of CWD, highlight the complexities associated with CWD transmission among cervids in their natural environment, emphasize the potential utility of blood-based testing to detect pre-clinical CWD infection, and could augur similar transmission dynamics in other prion infections.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT
Key to understanding the epidemiology and pathogenesis of prion diseases, including chronic wasting disease (CWD) of cervids, is determining the mode of transmission from one individual to another. We have previously reported that saliva and blood from CWD-infected deer contain sufficient infectious prions to transmit disease upon passage into naïve deer. Here we again use bioassays in deer to show that blood and saliva of pre-symptomatic deer contain infectious prions capable of infecting naïve deer and that naïve deer exposed only to environmental fomites from the suites of CWD-infected deer acquired CWD infection after a period of 15 months post initial exposure. These results help to further explain the basis for the facile transmission of CWD, highlight the complexities associated with CWD transmission among cervids in their natural environment, emphasize the potential utility of blood-based testing to detect pre-clinical CWD infection, and could augur similar transmission dynamics in other prion infections.

Show MeSH
Related in: MedlinePlus