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Expression of novel p53 isoforms in oral lichen planus.

Ebrahimi M, Boldrup L, Coates PJ, Wahlin YB, Bourdon JC, Nylander K - Oral Oncol. (2007)

Bottom Line: In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions.The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples.Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Odontology, Umeå University, SE - 901 85 Umeå, Sweden. majid.ebrahimi@medbio.umu.se <majid.ebrahimi@medbio.umu.se>

ABSTRACT
Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin, showing little spontaneous regression. WHO classifies OLP as a premalignant condition, however, the underlying mechanisms initiating development of cancer in OLP lesions are not understood. The p53 tumour suppressor plays an important role in many tumours, and an increased expression of p53 protein has been seen in OLP lesions. Recently it was shown that the human TP53 gene encodes at least nine different isoforms. Another member of the p53 family, p63, comprises six different isoforms and plays a crucial role in the formation of oral mucosa, salivary glands, teeth and skin. It has also been suggested that p63 is involved in development of squamous cell carcinoma of the head and neck (SCCHN). In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions. In this study, we mapped the expression of five novel p53 isoforms at RNA and protein levels in OLP and matched normal controls. In the same samples we also measured levels of p63 isoforms using quantitative RT-PCR. Results showed p53 to be expressed in all OLP lesions and normal tissues. The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples. Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

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Quantitative RT/PCR of the different p63 isoforms in OLP lesions and controls. Black bars represent OLP lesions and white bars normal controls. Results are expressed as mean for all samples analysed in each group and are based on the relative amount of p63 RNA for each sample analysed adjusted for the level of internal control amplification (β-actin) obtained for each sample. The most pronounced decrease was seen in levels of ΔNp63, p63α and p63γ. Levels of TAp63 were basically unchanged when comparing OLP lesions and controls, while a slight reduction in p63β was seen in OLP. Note that different scales are used for the two groups TAp63, p63β, p63γ and ΔNp63, p63α, respectively.
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fig2: Quantitative RT/PCR of the different p63 isoforms in OLP lesions and controls. Black bars represent OLP lesions and white bars normal controls. Results are expressed as mean for all samples analysed in each group and are based on the relative amount of p63 RNA for each sample analysed adjusted for the level of internal control amplification (β-actin) obtained for each sample. The most pronounced decrease was seen in levels of ΔNp63, p63α and p63γ. Levels of TAp63 were basically unchanged when comparing OLP lesions and controls, while a slight reduction in p63β was seen in OLP. Note that different scales are used for the two groups TAp63, p63β, p63γ and ΔNp63, p63α, respectively.

Mentions: Using quantitative RT–PCR analysis of p63 showed that p63α had highest and ΔNp63 second highest level of all p63 mRNAs. TAp63 had lowest levels in both OLP and corresponding normal tissue. For all isoforms individual variations could be seen (results not shown). Results also showed a general decrease in levels of p63 isoforms in OLP compared to normal tissue. The most pronounced decrease seen in expression of p63γ, p63α and ΔNp63 (P = 0.116; 0.187 and 0.205, respectively). The level of TAp63 was unchanged while a slight reduction in p63β was found in OLP lesions compared to normal tissue. None of these changes were, however, statistically significant (Fig. 2).


Expression of novel p53 isoforms in oral lichen planus.

Ebrahimi M, Boldrup L, Coates PJ, Wahlin YB, Bourdon JC, Nylander K - Oral Oncol. (2007)

Quantitative RT/PCR of the different p63 isoforms in OLP lesions and controls. Black bars represent OLP lesions and white bars normal controls. Results are expressed as mean for all samples analysed in each group and are based on the relative amount of p63 RNA for each sample analysed adjusted for the level of internal control amplification (β-actin) obtained for each sample. The most pronounced decrease was seen in levels of ΔNp63, p63α and p63γ. Levels of TAp63 were basically unchanged when comparing OLP lesions and controls, while a slight reduction in p63β was seen in OLP. Note that different scales are used for the two groups TAp63, p63β, p63γ and ΔNp63, p63α, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2691586&req=5

fig2: Quantitative RT/PCR of the different p63 isoforms in OLP lesions and controls. Black bars represent OLP lesions and white bars normal controls. Results are expressed as mean for all samples analysed in each group and are based on the relative amount of p63 RNA for each sample analysed adjusted for the level of internal control amplification (β-actin) obtained for each sample. The most pronounced decrease was seen in levels of ΔNp63, p63α and p63γ. Levels of TAp63 were basically unchanged when comparing OLP lesions and controls, while a slight reduction in p63β was seen in OLP. Note that different scales are used for the two groups TAp63, p63β, p63γ and ΔNp63, p63α, respectively.
Mentions: Using quantitative RT–PCR analysis of p63 showed that p63α had highest and ΔNp63 second highest level of all p63 mRNAs. TAp63 had lowest levels in both OLP and corresponding normal tissue. For all isoforms individual variations could be seen (results not shown). Results also showed a general decrease in levels of p63 isoforms in OLP compared to normal tissue. The most pronounced decrease seen in expression of p63γ, p63α and ΔNp63 (P = 0.116; 0.187 and 0.205, respectively). The level of TAp63 was unchanged while a slight reduction in p63β was found in OLP lesions compared to normal tissue. None of these changes were, however, statistically significant (Fig. 2).

Bottom Line: In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions.The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples.Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Odontology, Umeå University, SE - 901 85 Umeå, Sweden. majid.ebrahimi@medbio.umu.se <majid.ebrahimi@medbio.umu.se>

ABSTRACT
Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin, showing little spontaneous regression. WHO classifies OLP as a premalignant condition, however, the underlying mechanisms initiating development of cancer in OLP lesions are not understood. The p53 tumour suppressor plays an important role in many tumours, and an increased expression of p53 protein has been seen in OLP lesions. Recently it was shown that the human TP53 gene encodes at least nine different isoforms. Another member of the p53 family, p63, comprises six different isoforms and plays a crucial role in the formation of oral mucosa, salivary glands, teeth and skin. It has also been suggested that p63 is involved in development of squamous cell carcinoma of the head and neck (SCCHN). In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions. In this study, we mapped the expression of five novel p53 isoforms at RNA and protein levels in OLP and matched normal controls. In the same samples we also measured levels of p63 isoforms using quantitative RT-PCR. Results showed p53 to be expressed in all OLP lesions and normal tissues. The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples. Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

Show MeSH
Related in: MedlinePlus