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Expression of novel p53 isoforms in oral lichen planus.

Ebrahimi M, Boldrup L, Coates PJ, Wahlin YB, Bourdon JC, Nylander K - Oral Oncol. (2007)

Bottom Line: In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions.The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples.Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Odontology, Umeå University, SE - 901 85 Umeå, Sweden. majid.ebrahimi@medbio.umu.se <majid.ebrahimi@medbio.umu.se>

ABSTRACT
Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin, showing little spontaneous regression. WHO classifies OLP as a premalignant condition, however, the underlying mechanisms initiating development of cancer in OLP lesions are not understood. The p53 tumour suppressor plays an important role in many tumours, and an increased expression of p53 protein has been seen in OLP lesions. Recently it was shown that the human TP53 gene encodes at least nine different isoforms. Another member of the p53 family, p63, comprises six different isoforms and plays a crucial role in the formation of oral mucosa, salivary glands, teeth and skin. It has also been suggested that p63 is involved in development of squamous cell carcinoma of the head and neck (SCCHN). In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions. In this study, we mapped the expression of five novel p53 isoforms at RNA and protein levels in OLP and matched normal controls. In the same samples we also measured levels of p63 isoforms using quantitative RT-PCR. Results showed p53 to be expressed in all OLP lesions and normal tissues. The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples. Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

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Results from nested RT–PCR analysis of six p53 isoforms. Bars show percentage of positive samples. In total 40 samples were analysed, 20 OLP lesions and 20 sex and aged matched normal tissues. P53 mRNA was detected in all OLP lesions and normal tissue, either in all replicates analysed (positive) or in one or two of the replicates (positive/variable). Of the five novel p53 isoforms analysed, p53β and Δ133p53 were detected in the majority of samples, while the remaining three novel isoforms only were detected in a few samples.
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fig1: Results from nested RT–PCR analysis of six p53 isoforms. Bars show percentage of positive samples. In total 40 samples were analysed, 20 OLP lesions and 20 sex and aged matched normal tissues. P53 mRNA was detected in all OLP lesions and normal tissue, either in all replicates analysed (positive) or in one or two of the replicates (positive/variable). Of the five novel p53 isoforms analysed, p53β and Δ133p53 were detected in the majority of samples, while the remaining three novel isoforms only were detected in a few samples.

Mentions: By the use of RT–PCR, six different p53 isoforms could be amplified from OLP tissue as well as normal tissue: p53, p53β, p53γ, Δ133p53, Δ133p53β, and Δ133p53γ. All samples were analysed as matched pairs, meaning that one OLP lesion and corresponding matched control were analysed in parallel, though coded, as to hide which sample was lesion and which was control. p53 mRNA could be detected in replicate analyses of all but 1 of the 20 OLP samples as well as all but 1 of the 20 controls. These two samples were positive in one analysis, but negative in the other. The majority of OLP and control samples also expressed the p53β and Δ133p53 isoforms in at least one of the replicate analyses. Expression of p53γ, Δ133p53β and Δ133p53γ was, however, limited to only a few samples. In general the novel p53 isoforms studied were detected in fewer OLP samples compared to matched controls (Fig. 1).


Expression of novel p53 isoforms in oral lichen planus.

Ebrahimi M, Boldrup L, Coates PJ, Wahlin YB, Bourdon JC, Nylander K - Oral Oncol. (2007)

Results from nested RT–PCR analysis of six p53 isoforms. Bars show percentage of positive samples. In total 40 samples were analysed, 20 OLP lesions and 20 sex and aged matched normal tissues. P53 mRNA was detected in all OLP lesions and normal tissue, either in all replicates analysed (positive) or in one or two of the replicates (positive/variable). Of the five novel p53 isoforms analysed, p53β and Δ133p53 were detected in the majority of samples, while the remaining three novel isoforms only were detected in a few samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2691586&req=5

fig1: Results from nested RT–PCR analysis of six p53 isoforms. Bars show percentage of positive samples. In total 40 samples were analysed, 20 OLP lesions and 20 sex and aged matched normal tissues. P53 mRNA was detected in all OLP lesions and normal tissue, either in all replicates analysed (positive) or in one or two of the replicates (positive/variable). Of the five novel p53 isoforms analysed, p53β and Δ133p53 were detected in the majority of samples, while the remaining three novel isoforms only were detected in a few samples.
Mentions: By the use of RT–PCR, six different p53 isoforms could be amplified from OLP tissue as well as normal tissue: p53, p53β, p53γ, Δ133p53, Δ133p53β, and Δ133p53γ. All samples were analysed as matched pairs, meaning that one OLP lesion and corresponding matched control were analysed in parallel, though coded, as to hide which sample was lesion and which was control. p53 mRNA could be detected in replicate analyses of all but 1 of the 20 OLP samples as well as all but 1 of the 20 controls. These two samples were positive in one analysis, but negative in the other. The majority of OLP and control samples also expressed the p53β and Δ133p53 isoforms in at least one of the replicate analyses. Expression of p53γ, Δ133p53β and Δ133p53γ was, however, limited to only a few samples. In general the novel p53 isoforms studied were detected in fewer OLP samples compared to matched controls (Fig. 1).

Bottom Line: In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions.The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples.Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

View Article: PubMed Central - PubMed

Affiliation: Department of Odontology, Umeå University, SE - 901 85 Umeå, Sweden. majid.ebrahimi@medbio.umu.se <majid.ebrahimi@medbio.umu.se>

ABSTRACT
Oral lichen planus (OLP) is a chronic inflammatory disease of unknown origin, showing little spontaneous regression. WHO classifies OLP as a premalignant condition, however, the underlying mechanisms initiating development of cancer in OLP lesions are not understood. The p53 tumour suppressor plays an important role in many tumours, and an increased expression of p53 protein has been seen in OLP lesions. Recently it was shown that the human TP53 gene encodes at least nine different isoforms. Another member of the p53 family, p63, comprises six different isoforms and plays a crucial role in the formation of oral mucosa, salivary glands, teeth and skin. It has also been suggested that p63 is involved in development of squamous cell carcinoma of the head and neck (SCCHN). In contrast to p53, a decreased expression of p63 protein has been seen in OLP lesions. In this study, we mapped the expression of five novel p53 isoforms at RNA and protein levels in OLP and matched normal controls. In the same samples we also measured levels of p63 isoforms using quantitative RT-PCR. Results showed p53 to be expressed in all OLP lesions and normal tissues. The p53 beta and delta 133p53 isoforms were expressed in the majority of samples whereas the remaining three novel isoforms analysed were expressed in only a few samples. Levels of p63 isoforms were lower in OLP lesions compared with normal tissue, however, changes were not statistically significant.

Show MeSH
Related in: MedlinePlus