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Comprehensive behavioral phenotyping of ryanodine receptor type 3 (RyR3) knockout mice: decreased social contact duration in two social interaction tests.

Matsuo N, Tanda K, Nakanishi K, Yamasaki N, Toyama K, Takao K, Takeshima H, Miyakawa T - Front Behav Neurosci (2009)

Bottom Line: Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum.They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests.These results indicate that RyR3 has an important role in locomotor activity and social behavior.

View Article: PubMed Central - PubMed

Affiliation: Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University Toyoake, Japan.

ABSTRACT
Dynamic regulation of the intracellular Ca2+ concentration is crucial for various neuronal functions such as synaptic transmission and plasticity, and gene expression. Ryanodine receptors (RyRs) are a family of intracellular calcium release channels that mediate calcium-induced calcium release from the endoplasmic reticulum. Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum. To investigate the behavioral effects of RyR3 deficiency, we subjected RyR3 knockout (RyR3-/-) mice to a battery of behavioral tests. RyR3-/- mice exhibited significantly decreased social contact duration in two different social interaction tests, where two mice can freely move and make contacts with each other. They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests. These results indicate that RyR3 has an important role in locomotor activity and social behavior.

No MeSH data available.


Related in: MedlinePlus

Increased locomotor activity of RyR3–/– mice in the open field test. (A) Total locomotion distance was significantly increased in the KO mice. (B) Count of vertical activity. (C) Count of stereotypic behavior. (D) Time spent in the center of the compartment. WT and KO stand for RyR3+/+ and RyR3–/– mice, respectively.
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Figure 2: Increased locomotor activity of RyR3–/– mice in the open field test. (A) Total locomotion distance was significantly increased in the KO mice. (B) Count of vertical activity. (C) Count of stereotypic behavior. (D) Time spent in the center of the compartment. WT and KO stand for RyR3+/+ and RyR3–/– mice, respectively.

Mentions: Examination of the locomotor activity of RyR3–/– mice in several behavioral tasks consistently revealed a hyperlocomotor activity phenotype. Total distance traveled by RyR3–/– mice was significantly greater than that of controls during an open field test (Figure 2A; F1,158 = 18.945, p < 0.0001), light–dark transition test (Figure 3A; dark: F1,171 = 69.155, light: F1,171 = 18.083, p < 0.0001), elevated plus-maze test (Figure 4A; F1,83 = 8.864, p = 0.0038), and the social interaction test in a novel environment (Figure 6E; F1,80 = 39.033, p < 0.0001). The number of transitions in the light–dark transition test (Figure 3B; F1,171 = 10.440, p = 0.0015) and the number of total entries in the elevated plus-maze test (Figure 4B; F1,83 = 12.707, p = 0.0006) of the RyR3–/– mice were also significantly higher than those of controls. Vertical activity and stereotypic counts did not differ between the genotypes in the open field test (Figures 2B,C; p = 0.8906, and 0.5725, respectively). Even in the homecage, which is not a novel environment, the activity level of RyR3–/– mice was significantly greater relative to controls during the dark phase of the circadian cycle (Figure 7A; F1,30 = 10.046, p = 0.0035), indicating that the hyperactivity phenotype is caused by an increased general locomotor activity rather than an increased response to novelty.


Comprehensive behavioral phenotyping of ryanodine receptor type 3 (RyR3) knockout mice: decreased social contact duration in two social interaction tests.

Matsuo N, Tanda K, Nakanishi K, Yamasaki N, Toyama K, Takao K, Takeshima H, Miyakawa T - Front Behav Neurosci (2009)

Increased locomotor activity of RyR3–/– mice in the open field test. (A) Total locomotion distance was significantly increased in the KO mice. (B) Count of vertical activity. (C) Count of stereotypic behavior. (D) Time spent in the center of the compartment. WT and KO stand for RyR3+/+ and RyR3–/– mice, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2691151&req=5

Figure 2: Increased locomotor activity of RyR3–/– mice in the open field test. (A) Total locomotion distance was significantly increased in the KO mice. (B) Count of vertical activity. (C) Count of stereotypic behavior. (D) Time spent in the center of the compartment. WT and KO stand for RyR3+/+ and RyR3–/– mice, respectively.
Mentions: Examination of the locomotor activity of RyR3–/– mice in several behavioral tasks consistently revealed a hyperlocomotor activity phenotype. Total distance traveled by RyR3–/– mice was significantly greater than that of controls during an open field test (Figure 2A; F1,158 = 18.945, p < 0.0001), light–dark transition test (Figure 3A; dark: F1,171 = 69.155, light: F1,171 = 18.083, p < 0.0001), elevated plus-maze test (Figure 4A; F1,83 = 8.864, p = 0.0038), and the social interaction test in a novel environment (Figure 6E; F1,80 = 39.033, p < 0.0001). The number of transitions in the light–dark transition test (Figure 3B; F1,171 = 10.440, p = 0.0015) and the number of total entries in the elevated plus-maze test (Figure 4B; F1,83 = 12.707, p = 0.0006) of the RyR3–/– mice were also significantly higher than those of controls. Vertical activity and stereotypic counts did not differ between the genotypes in the open field test (Figures 2B,C; p = 0.8906, and 0.5725, respectively). Even in the homecage, which is not a novel environment, the activity level of RyR3–/– mice was significantly greater relative to controls during the dark phase of the circadian cycle (Figure 7A; F1,30 = 10.046, p = 0.0035), indicating that the hyperactivity phenotype is caused by an increased general locomotor activity rather than an increased response to novelty.

Bottom Line: Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum.They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests.These results indicate that RyR3 has an important role in locomotor activity and social behavior.

View Article: PubMed Central - PubMed

Affiliation: Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University Toyoake, Japan.

ABSTRACT
Dynamic regulation of the intracellular Ca2+ concentration is crucial for various neuronal functions such as synaptic transmission and plasticity, and gene expression. Ryanodine receptors (RyRs) are a family of intracellular calcium release channels that mediate calcium-induced calcium release from the endoplasmic reticulum. Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum. To investigate the behavioral effects of RyR3 deficiency, we subjected RyR3 knockout (RyR3-/-) mice to a battery of behavioral tests. RyR3-/- mice exhibited significantly decreased social contact duration in two different social interaction tests, where two mice can freely move and make contacts with each other. They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests. These results indicate that RyR3 has an important role in locomotor activity and social behavior.

No MeSH data available.


Related in: MedlinePlus