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Comprehensive behavioral phenotyping of ryanodine receptor type 3 (RyR3) knockout mice: decreased social contact duration in two social interaction tests.

Matsuo N, Tanda K, Nakanishi K, Yamasaki N, Toyama K, Takao K, Takeshima H, Miyakawa T - Front Behav Neurosci (2009)

Bottom Line: Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum.They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests.These results indicate that RyR3 has an important role in locomotor activity and social behavior.

View Article: PubMed Central - PubMed

Affiliation: Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University Toyoake, Japan.

ABSTRACT
Dynamic regulation of the intracellular Ca2+ concentration is crucial for various neuronal functions such as synaptic transmission and plasticity, and gene expression. Ryanodine receptors (RyRs) are a family of intracellular calcium release channels that mediate calcium-induced calcium release from the endoplasmic reticulum. Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum. To investigate the behavioral effects of RyR3 deficiency, we subjected RyR3 knockout (RyR3-/-) mice to a battery of behavioral tests. RyR3-/- mice exhibited significantly decreased social contact duration in two different social interaction tests, where two mice can freely move and make contacts with each other. They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests. These results indicate that RyR3 has an important role in locomotor activity and social behavior.

No MeSH data available.


Related in: MedlinePlus

Normal physical characteristics of RyR3–/– mice. (A) Body weight. (B) Body temperature. (C) Wire hang test. (D) Grip strength test. (E) Hot plate test. (F) Rotarod test.
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Figure 1: Normal physical characteristics of RyR3–/– mice. (A) Body weight. (B) Body temperature. (C) Wire hang test. (D) Grip strength test. (E) Hot plate test. (F) Rotarod test.

Mentions: To address the behavioral effects of RyR3 deficiency, we subjected RyR3–/– mice and their wild-type littermates to a comprehensive battery of behavioral tests (Takao and Miyakawa, 2006a; Takao et al., 2007). RyR3–/– mice appeared healthy and showed no obvious differences in physical characteristics, with the exception of a slight decrease in body weight relative to wild-type mice (Figure 1A; F1,101 = 5.397, p = 0.0224). There were no significant differences in body temperature (Figure 1B; F1,101 = 0.001, p = 0.9808), neuromuscular strength (wire hang and grip strength tests) (Figures 1C,D; F1,101 = 0.239, p = 0.6257, and F1,101 = 0.842, p = 0.3610, respectively), sensitivity to a painful stimulus (hot plate test) (Figure 1E; F1,45 = 2.717, p = 0.1062), or motor functions (rotarod test) (Figure 1F; F1,45 = 0.243, p = 0.6246) between the wild-type and the knockout mice.


Comprehensive behavioral phenotyping of ryanodine receptor type 3 (RyR3) knockout mice: decreased social contact duration in two social interaction tests.

Matsuo N, Tanda K, Nakanishi K, Yamasaki N, Toyama K, Takao K, Takeshima H, Miyakawa T - Front Behav Neurosci (2009)

Normal physical characteristics of RyR3–/– mice. (A) Body weight. (B) Body temperature. (C) Wire hang test. (D) Grip strength test. (E) Hot plate test. (F) Rotarod test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2691151&req=5

Figure 1: Normal physical characteristics of RyR3–/– mice. (A) Body weight. (B) Body temperature. (C) Wire hang test. (D) Grip strength test. (E) Hot plate test. (F) Rotarod test.
Mentions: To address the behavioral effects of RyR3 deficiency, we subjected RyR3–/– mice and their wild-type littermates to a comprehensive battery of behavioral tests (Takao and Miyakawa, 2006a; Takao et al., 2007). RyR3–/– mice appeared healthy and showed no obvious differences in physical characteristics, with the exception of a slight decrease in body weight relative to wild-type mice (Figure 1A; F1,101 = 5.397, p = 0.0224). There were no significant differences in body temperature (Figure 1B; F1,101 = 0.001, p = 0.9808), neuromuscular strength (wire hang and grip strength tests) (Figures 1C,D; F1,101 = 0.239, p = 0.6257, and F1,101 = 0.842, p = 0.3610, respectively), sensitivity to a painful stimulus (hot plate test) (Figure 1E; F1,45 = 2.717, p = 0.1062), or motor functions (rotarod test) (Figure 1F; F1,45 = 0.243, p = 0.6246) between the wild-type and the knockout mice.

Bottom Line: Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum.They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests.These results indicate that RyR3 has an important role in locomotor activity and social behavior.

View Article: PubMed Central - PubMed

Affiliation: Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University Toyoake, Japan.

ABSTRACT
Dynamic regulation of the intracellular Ca2+ concentration is crucial for various neuronal functions such as synaptic transmission and plasticity, and gene expression. Ryanodine receptors (RyRs) are a family of intracellular calcium release channels that mediate calcium-induced calcium release from the endoplasmic reticulum. Among the three RyR isoforms, RyR3 is preferentially expressed in the brain especially in the hippocampus and striatum. To investigate the behavioral effects of RyR3 deficiency, we subjected RyR3 knockout (RyR3-/-) mice to a battery of behavioral tests. RyR3-/- mice exhibited significantly decreased social contact duration in two different social interaction tests, where two mice can freely move and make contacts with each other. They also exhibited hyperactivity and mildly impaired prepulse inhibition and latent inhibition while they did not show significant abnormalities in motor function and working and reference memory tests. These results indicate that RyR3 has an important role in locomotor activity and social behavior.

No MeSH data available.


Related in: MedlinePlus