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A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis.

Finkers-Tomczak A, Danan S, van Dijk T, Beyene A, Bouwman L, Overmars H, van Eck H, Goverse A, Bakker J, Bakker E - Theor. Appl. Genet. (2009)

Bottom Line: A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes.Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432.It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nematology, Plant Science Group, Wageningen University, Wageningen, The Netherlands.

ABSTRACT
The Grp1 locus confers broad-spectrum resistance to the potato cyst nematode species Globodera pallida and Globodera rostochiensis and is located in the GP21-GP179 interval on the short arm of chromosome V of potato. A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes. The flanking markers GP21 and GP179 have been used to screen the 1,536 genotypes for recombination events. Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432. Detailed analysis of the 44 recombinant genotypes showed that G. pallida and G. rostochiensis resistance could not be separated and map to the same location between marker SPUD838 and TG432. It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

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LOD graphs of G. rostochiensis Ro5 resistance (dashed line) and G. pallida Pa2 resistance (continuous line). The vertical axis represents the LOD score and the horizontal axis represents the genetic map. The threshold LOD of 2.5 is indicated by the dotted horizontal line
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Fig2: LOD graphs of G. rostochiensis Ro5 resistance (dashed line) and G. pallida Pa2 resistance (continuous line). The vertical axis represents the LOD score and the horizontal axis represents the genetic map. The threshold LOD of 2.5 is indicated by the dotted horizontal line

Mentions: Analysis of variance on normalised cyst counts revealed that the genetic variance for both G. pallida and G. rostochiensis resistance was significant (P < 0.0001). The log-transformed values of the averaged number of cysts per genotype showed a continuous distribution skewed towards susceptibility for both nematode populations. The broad-sense heritability was estimated to be 0.95 for G. rostochiensis and 0.93 for G. pallida. Interval mapping in the GP21–GP179 region was applied on the average number of cysts per genotype. For both G. pallida and G. rostochiensis, a significant QTL was detected with a maximum LOD score of 9.03 and 7.90, respectively. The QTL for G. pallida resistance explains 67% of total G. pallida resistance and the QTL for G. rostochiensis resistance explains 62% of total G. rostochiensis resistance. LOD score graphs for G. rostochiensis and G. pallida resistance are presented in Fig. 2.Fig. 2


A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis.

Finkers-Tomczak A, Danan S, van Dijk T, Beyene A, Bouwman L, Overmars H, van Eck H, Goverse A, Bakker J, Bakker E - Theor. Appl. Genet. (2009)

LOD graphs of G. rostochiensis Ro5 resistance (dashed line) and G. pallida Pa2 resistance (continuous line). The vertical axis represents the LOD score and the horizontal axis represents the genetic map. The threshold LOD of 2.5 is indicated by the dotted horizontal line
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2690855&req=5

Fig2: LOD graphs of G. rostochiensis Ro5 resistance (dashed line) and G. pallida Pa2 resistance (continuous line). The vertical axis represents the LOD score and the horizontal axis represents the genetic map. The threshold LOD of 2.5 is indicated by the dotted horizontal line
Mentions: Analysis of variance on normalised cyst counts revealed that the genetic variance for both G. pallida and G. rostochiensis resistance was significant (P < 0.0001). The log-transformed values of the averaged number of cysts per genotype showed a continuous distribution skewed towards susceptibility for both nematode populations. The broad-sense heritability was estimated to be 0.95 for G. rostochiensis and 0.93 for G. pallida. Interval mapping in the GP21–GP179 region was applied on the average number of cysts per genotype. For both G. pallida and G. rostochiensis, a significant QTL was detected with a maximum LOD score of 9.03 and 7.90, respectively. The QTL for G. pallida resistance explains 67% of total G. pallida resistance and the QTL for G. rostochiensis resistance explains 62% of total G. rostochiensis resistance. LOD score graphs for G. rostochiensis and G. pallida resistance are presented in Fig. 2.Fig. 2

Bottom Line: A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes.Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432.It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nematology, Plant Science Group, Wageningen University, Wageningen, The Netherlands.

ABSTRACT
The Grp1 locus confers broad-spectrum resistance to the potato cyst nematode species Globodera pallida and Globodera rostochiensis and is located in the GP21-GP179 interval on the short arm of chromosome V of potato. A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes. The flanking markers GP21 and GP179 have been used to screen the 1,536 genotypes for recombination events. Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432. Detailed analysis of the 44 recombinant genotypes showed that G. pallida and G. rostochiensis resistance could not be separated and map to the same location between marker SPUD838 and TG432. It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

Show MeSH
Related in: MedlinePlus