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A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis.

Finkers-Tomczak A, Danan S, van Dijk T, Beyene A, Bouwman L, Overmars H, van Eck H, Goverse A, Bakker J, Bakker E - Theor. Appl. Genet. (2009)

Bottom Line: A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes.Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432.It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nematology, Plant Science Group, Wageningen University, Wageningen, The Netherlands.

ABSTRACT
The Grp1 locus confers broad-spectrum resistance to the potato cyst nematode species Globodera pallida and Globodera rostochiensis and is located in the GP21-GP179 interval on the short arm of chromosome V of potato. A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes. The flanking markers GP21 and GP179 have been used to screen the 1,536 genotypes for recombination events. Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432. Detailed analysis of the 44 recombinant genotypes showed that G. pallida and G. rostochiensis resistance could not be separated and map to the same location between marker SPUD838 and TG432. It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

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Related in: MedlinePlus

Fine mapping of the GP21–GP179 interval on chromosome V of the diploid potato clone AM. Markers are presented in the correct genetic order. SPUD839, TG432, BA47F2, SD1, BA87D17, P3F8, BA43A11, SD4, SD5, SPUD1636, SD7 and GP179 are in coupling with GP21, and SD2, BA213C14, BA76O11, SD3 and SD6 are in repulsion with GP21. Bold horizontal lines represent chromosomal regions derived from the haplotype harbouring GP21 and thin horizontal lines represent chromosomal regions derived from the other haplotype. In the column on the right, the number of genotypes for each recombination event is given
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Fig1: Fine mapping of the GP21–GP179 interval on chromosome V of the diploid potato clone AM. Markers are presented in the correct genetic order. SPUD839, TG432, BA47F2, SD1, BA87D17, P3F8, BA43A11, SD4, SD5, SPUD1636, SD7 and GP179 are in coupling with GP21, and SD2, BA213C14, BA76O11, SD3 and SD6 are in repulsion with GP21. Bold horizontal lines represent chromosomal regions derived from the haplotype harbouring GP21 and thin horizontal lines represent chromosomal regions derived from the other haplotype. In the column on the right, the number of genotypes for each recombination event is given

Mentions: To identify markers closely linked to the Grp1 locus, primers were designed on sequences derived from this locus in other potato genotypes that were retrieved from Genbank and the GABI database. Thirteen primer combinations resulted in a segregating the AM allele. Table 1 presents the markers in more detail. Five markers were AM allele specific, showing the presence or absence of the amplification product. Eight markers were polymorphic after digestion with an appropriate enzyme (CAPS markers). In addition, polymorphisms were found for markers SPUD839 and TG432 (De Jong et al. 1997) and for SPUD1636 (Bryan et al. 2002). The 16 markers that revealed a polymorphism between AM and RH were subsequently tested on a subset of 44 genotypes that showed a recombination event in the GP21–GP179 interval. All markers were placed between GP21 and GP179 (Fig. 1). Some markers were not separated by recombination events and were grouped together.Fig. 1


A high-resolution map of the Grp1 locus on chromosome V of potato harbouring broad-spectrum resistance to the cyst nematode species Globodera pallida and Globodera rostochiensis.

Finkers-Tomczak A, Danan S, van Dijk T, Beyene A, Bouwman L, Overmars H, van Eck H, Goverse A, Bakker J, Bakker E - Theor. Appl. Genet. (2009)

Fine mapping of the GP21–GP179 interval on chromosome V of the diploid potato clone AM. Markers are presented in the correct genetic order. SPUD839, TG432, BA47F2, SD1, BA87D17, P3F8, BA43A11, SD4, SD5, SPUD1636, SD7 and GP179 are in coupling with GP21, and SD2, BA213C14, BA76O11, SD3 and SD6 are in repulsion with GP21. Bold horizontal lines represent chromosomal regions derived from the haplotype harbouring GP21 and thin horizontal lines represent chromosomal regions derived from the other haplotype. In the column on the right, the number of genotypes for each recombination event is given
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2690855&req=5

Fig1: Fine mapping of the GP21–GP179 interval on chromosome V of the diploid potato clone AM. Markers are presented in the correct genetic order. SPUD839, TG432, BA47F2, SD1, BA87D17, P3F8, BA43A11, SD4, SD5, SPUD1636, SD7 and GP179 are in coupling with GP21, and SD2, BA213C14, BA76O11, SD3 and SD6 are in repulsion with GP21. Bold horizontal lines represent chromosomal regions derived from the haplotype harbouring GP21 and thin horizontal lines represent chromosomal regions derived from the other haplotype. In the column on the right, the number of genotypes for each recombination event is given
Mentions: To identify markers closely linked to the Grp1 locus, primers were designed on sequences derived from this locus in other potato genotypes that were retrieved from Genbank and the GABI database. Thirteen primer combinations resulted in a segregating the AM allele. Table 1 presents the markers in more detail. Five markers were AM allele specific, showing the presence or absence of the amplification product. Eight markers were polymorphic after digestion with an appropriate enzyme (CAPS markers). In addition, polymorphisms were found for markers SPUD839 and TG432 (De Jong et al. 1997) and for SPUD1636 (Bryan et al. 2002). The 16 markers that revealed a polymorphism between AM and RH were subsequently tested on a subset of 44 genotypes that showed a recombination event in the GP21–GP179 interval. All markers were placed between GP21 and GP179 (Fig. 1). Some markers were not separated by recombination events and were grouped together.Fig. 1

Bottom Line: A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes.Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432.It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Nematology, Plant Science Group, Wageningen University, Wageningen, The Netherlands.

ABSTRACT
The Grp1 locus confers broad-spectrum resistance to the potato cyst nematode species Globodera pallida and Globodera rostochiensis and is located in the GP21-GP179 interval on the short arm of chromosome V of potato. A high-resolution map has been developed using the diploid mapping population RHAM026, comprising 1,536 genotypes. The flanking markers GP21 and GP179 have been used to screen the 1,536 genotypes for recombination events. Interval mapping of the resistances to G. pallida Pa2 and G. rostochiensis Ro5 resulted in two nearly identical LOD graphs with the highest LOD score just north of marker TG432. Detailed analysis of the 44 recombinant genotypes showed that G. pallida and G. rostochiensis resistance could not be separated and map to the same location between marker SPUD838 and TG432. It is suggested that the quantitative resistance to both nematode species at the Grp1 locus is mediated by one or more tightly linked R genes that might belong to the NBS-LRR class.

Show MeSH
Related in: MedlinePlus