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Genetic analysis of pigmented tuber flesh in potato.

Zhang Y, Jung CS, De Jong WS - Theor. Appl. Genet. (2009)

Bottom Line: QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9.The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9.All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853-1901, USA.

ABSTRACT
Interest in anthocyanin-pigmented potato tuber flesh is increasing. To genetically map and characterize loci that influence this trait, diploid potato clone 10618-01, which has partially pigmented flesh, was crossed with diploid 320-02, which has white flesh. Almost all progeny exhibited purple coloration in the flesh, with some clones having only a small percentage of tissue pigmented, other clones having most tissue pigmented, and the majority of clones showing intermediate color phenotypes. The two parents and 228 progeny were genotyped with 493 AFLP, 8 CAPS, and 13 SSR markers. QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9. The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9. A CAPS marker based on this gene was used to evaluate a collection of 21 tetraploid potato clones with highly or fully pigmented red or purple flesh, as well as 53 cultivars with white or yellow flesh. All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

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Association between colored tuber flesh and a CAPS marker allele based on the potato homolog of Petunia an1. Genomic DNA was amplified with Stan1 primers (Table 1), restricted with Taq I, and electrophoresed through a 2% agarose gel. An arrow denotes the approximately 980 bp band present in all clones tested with red or purple tuber flesh
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Fig3: Association between colored tuber flesh and a CAPS marker allele based on the potato homolog of Petunia an1. Genomic DNA was amplified with Stan1 primers (Table 1), restricted with Taq I, and electrophoresed through a 2% agarose gel. An arrow denotes the approximately 980 bp band present in all clones tested with red or purple tuber flesh

Mentions: CAPS markers based on ans, chi, Stan1, and jaf13 were tested for possible relationship with pigmented flesh in a panel of diverse potato germplasm consisting of 21 tetraploid potato clones with red or purple flesh and 53 clones with white or yellow tuber flesh. The Stan1 CAPS marker revealed a common digestion product, about 980 bp in size, in all 21 of the clones with pigmented flesh (Fig. 3 and Table 3). This same digestion product was present in only 21 of 53 white- and yellow-fleshed clones (Fig. 3 and Table 3), suggesting that a common bHLH allele contributes toward, but is not sufficient, for the ability to accumulate anthocyanin in potato tuber flesh. No association with flesh color was observed with CAPS markers based on jaf13, ans or chi in the same panel.Fig. 3


Genetic analysis of pigmented tuber flesh in potato.

Zhang Y, Jung CS, De Jong WS - Theor. Appl. Genet. (2009)

Association between colored tuber flesh and a CAPS marker allele based on the potato homolog of Petunia an1. Genomic DNA was amplified with Stan1 primers (Table 1), restricted with Taq I, and electrophoresed through a 2% agarose gel. An arrow denotes the approximately 980 bp band present in all clones tested with red or purple tuber flesh
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2690854&req=5

Fig3: Association between colored tuber flesh and a CAPS marker allele based on the potato homolog of Petunia an1. Genomic DNA was amplified with Stan1 primers (Table 1), restricted with Taq I, and electrophoresed through a 2% agarose gel. An arrow denotes the approximately 980 bp band present in all clones tested with red or purple tuber flesh
Mentions: CAPS markers based on ans, chi, Stan1, and jaf13 were tested for possible relationship with pigmented flesh in a panel of diverse potato germplasm consisting of 21 tetraploid potato clones with red or purple flesh and 53 clones with white or yellow tuber flesh. The Stan1 CAPS marker revealed a common digestion product, about 980 bp in size, in all 21 of the clones with pigmented flesh (Fig. 3 and Table 3). This same digestion product was present in only 21 of 53 white- and yellow-fleshed clones (Fig. 3 and Table 3), suggesting that a common bHLH allele contributes toward, but is not sufficient, for the ability to accumulate anthocyanin in potato tuber flesh. No association with flesh color was observed with CAPS markers based on jaf13, ans or chi in the same panel.Fig. 3

Bottom Line: QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9.The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9.All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853-1901, USA.

ABSTRACT
Interest in anthocyanin-pigmented potato tuber flesh is increasing. To genetically map and characterize loci that influence this trait, diploid potato clone 10618-01, which has partially pigmented flesh, was crossed with diploid 320-02, which has white flesh. Almost all progeny exhibited purple coloration in the flesh, with some clones having only a small percentage of tissue pigmented, other clones having most tissue pigmented, and the majority of clones showing intermediate color phenotypes. The two parents and 228 progeny were genotyped with 493 AFLP, 8 CAPS, and 13 SSR markers. QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9. The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9. A CAPS marker based on this gene was used to evaluate a collection of 21 tetraploid potato clones with highly or fully pigmented red or purple flesh, as well as 53 cultivars with white or yellow flesh. All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

Show MeSH
Related in: MedlinePlus