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Genetic analysis of pigmented tuber flesh in potato.

Zhang Y, Jung CS, De Jong WS - Theor. Appl. Genet. (2009)

Bottom Line: QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9.The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9.All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853-1901, USA.

ABSTRACT
Interest in anthocyanin-pigmented potato tuber flesh is increasing. To genetically map and characterize loci that influence this trait, diploid potato clone 10618-01, which has partially pigmented flesh, was crossed with diploid 320-02, which has white flesh. Almost all progeny exhibited purple coloration in the flesh, with some clones having only a small percentage of tissue pigmented, other clones having most tissue pigmented, and the majority of clones showing intermediate color phenotypes. The two parents and 228 progeny were genotyped with 493 AFLP, 8 CAPS, and 13 SSR markers. QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9. The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9. A CAPS marker based on this gene was used to evaluate a collection of 21 tetraploid potato clones with highly or fully pigmented red or purple flesh, as well as 53 cultivars with white or yellow flesh. All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

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Location of QTLs that influenced extent of tuber flesh coloration in 2006. Map locations for anthocyanin-related genes chi, chs, and Stan1 are also shown
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Fig2: Location of QTLs that influenced extent of tuber flesh coloration in 2006. Map locations for anthocyanin-related genes chi, chs, and Stan1 are also shown

Mentions: Marker and year 2006 trait data were then analyzed using both nonparametric (Kruskal–Wallis) and parametric (interval mapping) approaches. Kruskal–Wallis analysis revealed significant (P < 0.001) loci on chromosome 5 of both parents: for 10618–01, at AFLP marker E32M49-442, and for 320–02, at marker E53M54-162 (Table 2). In addition, highly significant loci were detected on chromosome 8 of 320-02 at marker P14M37-134 (P < 0.0005) and on chromosome 9 of 10618-01 at marker E32M48-233 (P < 0.0001) (Table 2). QTLs at comparable locations were identified by interval mapping (Fig. 2, Table 2). The same loci were detected when phenotypic data for year 2007 was analyzed separately. Tuber pigmentation scores were not identical in 2006 and 2007, but were highly correlated (r2 = 0.61). Two additional loci were detected only in 2007, on chromosome 8 of 10618-01 at marker E32M48-318 (LOD 3.1) and chromosome 3 of 320-02 at marker E39M50-249 (also with LOD 3.1) (data not shown).Table 2


Genetic analysis of pigmented tuber flesh in potato.

Zhang Y, Jung CS, De Jong WS - Theor. Appl. Genet. (2009)

Location of QTLs that influenced extent of tuber flesh coloration in 2006. Map locations for anthocyanin-related genes chi, chs, and Stan1 are also shown
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2690854&req=5

Fig2: Location of QTLs that influenced extent of tuber flesh coloration in 2006. Map locations for anthocyanin-related genes chi, chs, and Stan1 are also shown
Mentions: Marker and year 2006 trait data were then analyzed using both nonparametric (Kruskal–Wallis) and parametric (interval mapping) approaches. Kruskal–Wallis analysis revealed significant (P < 0.001) loci on chromosome 5 of both parents: for 10618–01, at AFLP marker E32M49-442, and for 320–02, at marker E53M54-162 (Table 2). In addition, highly significant loci were detected on chromosome 8 of 320-02 at marker P14M37-134 (P < 0.0005) and on chromosome 9 of 10618-01 at marker E32M48-233 (P < 0.0001) (Table 2). QTLs at comparable locations were identified by interval mapping (Fig. 2, Table 2). The same loci were detected when phenotypic data for year 2007 was analyzed separately. Tuber pigmentation scores were not identical in 2006 and 2007, but were highly correlated (r2 = 0.61). Two additional loci were detected only in 2007, on chromosome 8 of 10618-01 at marker E32M48-318 (LOD 3.1) and chromosome 3 of 320-02 at marker E39M50-249 (also with LOD 3.1) (data not shown).Table 2

Bottom Line: QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9.The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9.All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY 14853-1901, USA.

ABSTRACT
Interest in anthocyanin-pigmented potato tuber flesh is increasing. To genetically map and characterize loci that influence this trait, diploid potato clone 10618-01, which has partially pigmented flesh, was crossed with diploid 320-02, which has white flesh. Almost all progeny exhibited purple coloration in the flesh, with some clones having only a small percentage of tissue pigmented, other clones having most tissue pigmented, and the majority of clones showing intermediate color phenotypes. The two parents and 228 progeny were genotyped with 493 AFLP, 8 CAPS, and 13 SSR markers. QTLs influencing extent of flesh pigmentation were detected on chromosomes 5, 8, and 9. The potato homolog of Petunia an1, a basic helix-loop-helix (bHLH) transcriptional regulator of anthocyanin biosynthesis, was found to co-localize with the QTL on chromosome 9. A CAPS marker based on this gene was used to evaluate a collection of 21 tetraploid potato clones with highly or fully pigmented red or purple flesh, as well as 53 cultivars with white or yellow flesh. All 21 pigmented-flesh clones shared a marker allele that was present in only 21 of the 53 white and yellow clones, suggesting that a common bHLH allele contributes toward, although it is clearly not sufficient for, highly or fully pigmented tuber flesh in cultivated potato.

Show MeSH
Related in: MedlinePlus