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Multivariate modeling of chromium-induced oxidative stress and biochemical changes in plants of Pistia stratiotes L.

Sinha S, Basant A, Malik A, Singh KP - Ecotoxicology (2009)

Bottom Line: Principal components analysis results suggested that malondialdehyde (MDA), superoxide dismutase (SOD), APX, non-protein thiols (NP-SH), cysteine, ascorbic acid, and Cr-accumulation are dominant in root tissues, whereas, protein and guaiacol peroxidase (GPX) in shoots of the plant.Discriminant partial least squares analysis results further confirmed that MDA, SOD, NP-SH, cysteine, GPX, APX, ascorbic acid and Cr-accumulation dominated in the root tissues, while protein in the shoot.The multivariate approaches, thus, allowed for the interpretation of the induced biochemical changes in the plant tissues exposed to chromium, which otherwise using the conventional approaches is difficult.

View Article: PubMed Central - PubMed

Affiliation: Ecotoxicology and Bioremediation Group, National Botanical Research Institute (Council of Scientific & Industrial Research), Rana Pratap Marg, Lucknow 226001, India. sinha_sarita52@yahoo.com

ABSTRACT
Biochemical changes in the plants of Pistia stratiotes L., a free floating macrophyte exposed to different concentrations of hexavalent chromium (0, 10, 40, 60, 80 and 160 microM) for 48, 96 and 144 h were studied. Chromium-induced oxidative stress in macrophyte was investigated using the multivariate modeling approaches. Cluster analysis rendered two fairly distinct clusters (roots and shoots) of similar characteristics in terms of their biochemical responses. Discriminant analysis identified ascorbate peroxidase (APX) as discriminating variable between the root and shoot tissues. Principal components analysis results suggested that malondialdehyde (MDA), superoxide dismutase (SOD), APX, non-protein thiols (NP-SH), cysteine, ascorbic acid, and Cr-accumulation are dominant in root tissues, whereas, protein and guaiacol peroxidase (GPX) in shoots of the plant. Discriminant partial least squares analysis results further confirmed that MDA, SOD, NP-SH, cysteine, GPX, APX, ascorbic acid and Cr-accumulation dominated in the root tissues, while protein in the shoot. Three-way analysis helped in visualizing simultaneous influence of metal concentration and exposure duration on biochemical variables in plant tissues. The multivariate approaches, thus, allowed for the interpretation of the induced biochemical changes in the plant tissues exposed to chromium, which otherwise using the conventional approaches is difficult.

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Plot of loadings values for the components extracted by Tucker3 model applied to the P. stratiotes data set for a metal concentrations (A1, A2, A3), b biochemical variables (B1, B2, B3), and c exposure time (C) mode
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Fig4: Plot of loadings values for the components extracted by Tucker3 model applied to the P. stratiotes data set for a metal concentrations (A1, A2, A3), b biochemical variables (B1, B2, B3), and c exposure time (C) mode

Mentions: Simultaneous influence of the chromium concentrations and exposure durations on biochemical changes in root and shoot tissues of the plant was studied through the multi-way component analysis of the data. The three-way data comprised of metal concentrations, biochemical variables and exposure durations was modeled using the Tucker3 model. Finally, the Tucker model of complexity, (3,3,1), that is three components each in first (concentrations) and second (biochemical variables) modes and single component in third mode (exposures) was selected, which captured 70% of the data variance. The loadings of metal concentrations mode (A), biochemical parameters (B) and exposure durations (C) are displayed in Fig. 4a–c.Fig. 4


Multivariate modeling of chromium-induced oxidative stress and biochemical changes in plants of Pistia stratiotes L.

Sinha S, Basant A, Malik A, Singh KP - Ecotoxicology (2009)

Plot of loadings values for the components extracted by Tucker3 model applied to the P. stratiotes data set for a metal concentrations (A1, A2, A3), b biochemical variables (B1, B2, B3), and c exposure time (C) mode
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2690848&req=5

Fig4: Plot of loadings values for the components extracted by Tucker3 model applied to the P. stratiotes data set for a metal concentrations (A1, A2, A3), b biochemical variables (B1, B2, B3), and c exposure time (C) mode
Mentions: Simultaneous influence of the chromium concentrations and exposure durations on biochemical changes in root and shoot tissues of the plant was studied through the multi-way component analysis of the data. The three-way data comprised of metal concentrations, biochemical variables and exposure durations was modeled using the Tucker3 model. Finally, the Tucker model of complexity, (3,3,1), that is three components each in first (concentrations) and second (biochemical variables) modes and single component in third mode (exposures) was selected, which captured 70% of the data variance. The loadings of metal concentrations mode (A), biochemical parameters (B) and exposure durations (C) are displayed in Fig. 4a–c.Fig. 4

Bottom Line: Principal components analysis results suggested that malondialdehyde (MDA), superoxide dismutase (SOD), APX, non-protein thiols (NP-SH), cysteine, ascorbic acid, and Cr-accumulation are dominant in root tissues, whereas, protein and guaiacol peroxidase (GPX) in shoots of the plant.Discriminant partial least squares analysis results further confirmed that MDA, SOD, NP-SH, cysteine, GPX, APX, ascorbic acid and Cr-accumulation dominated in the root tissues, while protein in the shoot.The multivariate approaches, thus, allowed for the interpretation of the induced biochemical changes in the plant tissues exposed to chromium, which otherwise using the conventional approaches is difficult.

View Article: PubMed Central - PubMed

Affiliation: Ecotoxicology and Bioremediation Group, National Botanical Research Institute (Council of Scientific & Industrial Research), Rana Pratap Marg, Lucknow 226001, India. sinha_sarita52@yahoo.com

ABSTRACT
Biochemical changes in the plants of Pistia stratiotes L., a free floating macrophyte exposed to different concentrations of hexavalent chromium (0, 10, 40, 60, 80 and 160 microM) for 48, 96 and 144 h were studied. Chromium-induced oxidative stress in macrophyte was investigated using the multivariate modeling approaches. Cluster analysis rendered two fairly distinct clusters (roots and shoots) of similar characteristics in terms of their biochemical responses. Discriminant analysis identified ascorbate peroxidase (APX) as discriminating variable between the root and shoot tissues. Principal components analysis results suggested that malondialdehyde (MDA), superoxide dismutase (SOD), APX, non-protein thiols (NP-SH), cysteine, ascorbic acid, and Cr-accumulation are dominant in root tissues, whereas, protein and guaiacol peroxidase (GPX) in shoots of the plant. Discriminant partial least squares analysis results further confirmed that MDA, SOD, NP-SH, cysteine, GPX, APX, ascorbic acid and Cr-accumulation dominated in the root tissues, while protein in the shoot. Three-way analysis helped in visualizing simultaneous influence of metal concentration and exposure duration on biochemical variables in plant tissues. The multivariate approaches, thus, allowed for the interpretation of the induced biochemical changes in the plant tissues exposed to chromium, which otherwise using the conventional approaches is difficult.

Show MeSH
Related in: MedlinePlus