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Chikungunya virus and Aedes mosquitoes: saliva is infectious as soon as two days after oral infection.

Dubrulle M, Mousson L, Moutailler S, Vazeille M, Failloux AB - PLoS ONE (2009)

Bottom Line: The recent CHIKV outbreaks were caused by a new variant characterized by a mutation in the E1 glycoprotein gene (E1-226V) which has favored a better transmissibility by Ae. albopictus.Ae. albopictus is slightly more efficient than Ae. aegypti to transmit the variant E1-226V of CHIKV.These results will help to design an efficient vector control to limit transmission as soon as the first human cases are diagnosed.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur, Génétique moléculaire des Bunyavirus, Paris, France.

ABSTRACT

Background: Aedes aegypti and Aedes albopictus are potential vectors of chikungunya virus (CHIKV). The recent CHIKV outbreaks were caused by a new variant characterized by a mutation in the E1 glycoprotein gene (E1-226V) which has favored a better transmissibility by Ae. albopictus. As Ae. albopictus tends to replace Ae. aegypti in many regions, one question remained: is Ae. albopictus as efficient as Ae. aegypti to transmit the variant E1-226V of CHIKV?

Methodology and findings: We infected orally both species with the variant E1-226V and estimated the infection, the viral dissemination, and the transmission rate by real time RT-PCR. Additionally, we used an in vitro assay to determine the amount of virus delivered by mosquitoes in their saliva. We found that Ae. aegypti as well as Ae. albopictus ensured a high replication of the virus which underwent an efficient dissemination as detectable in the salivary glands at day 2 post-infection (pi). Infectious CHIKV particles were delivered by salivary glands from day 2 with a maximum at day 6 pi for Ae. albopictus (10(3.3) PFU) and day 7 pi for Ae. aegypti (10(2.5) PFU).

Conclusions: Ae. albopictus is slightly more efficient than Ae. aegypti to transmit the variant E1-226V of CHIKV. These results will help to design an efficient vector control to limit transmission as soon as the first human cases are diagnosed.

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Related in: MedlinePlus

CHIKV dissemination in Aedes albopictus Providence (ALPROV) and Aedes aegypti Petite-Terre (AAPT).At different days after oral infection, viral dissemination was measured by quantification of viral RNA in mosquito wings of 5 females by real-time RT-PCR.
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pone-0005895-g002: CHIKV dissemination in Aedes albopictus Providence (ALPROV) and Aedes aegypti Petite-Terre (AAPT).At different days after oral infection, viral dissemination was measured by quantification of viral RNA in mosquito wings of 5 females by real-time RT-PCR.

Mentions: When examining viral dissemination inside female by RNA quantification in wings at different days after exposure to infectious meal, viral RNA started to be detectable at day 2 pi for both species: values ranged from 101.1 to 102.7 (101.1±101.3) for Ae. albopictus and from 102.0 to 103.3 (101.5±101.6) for Ae. aegypti (Figure 2). It reached a maximum (103.4–104.8 with 104±100.5) at day 7 pi for Ae. albopictus and at day 3 pi (102.0–105.3 with 103.7±101.4) for Ae. aegypti. Values were more variable between Ae. aegypti females at different days pi.


Chikungunya virus and Aedes mosquitoes: saliva is infectious as soon as two days after oral infection.

Dubrulle M, Mousson L, Moutailler S, Vazeille M, Failloux AB - PLoS ONE (2009)

CHIKV dissemination in Aedes albopictus Providence (ALPROV) and Aedes aegypti Petite-Terre (AAPT).At different days after oral infection, viral dissemination was measured by quantification of viral RNA in mosquito wings of 5 females by real-time RT-PCR.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2690823&req=5

pone-0005895-g002: CHIKV dissemination in Aedes albopictus Providence (ALPROV) and Aedes aegypti Petite-Terre (AAPT).At different days after oral infection, viral dissemination was measured by quantification of viral RNA in mosquito wings of 5 females by real-time RT-PCR.
Mentions: When examining viral dissemination inside female by RNA quantification in wings at different days after exposure to infectious meal, viral RNA started to be detectable at day 2 pi for both species: values ranged from 101.1 to 102.7 (101.1±101.3) for Ae. albopictus and from 102.0 to 103.3 (101.5±101.6) for Ae. aegypti (Figure 2). It reached a maximum (103.4–104.8 with 104±100.5) at day 7 pi for Ae. albopictus and at day 3 pi (102.0–105.3 with 103.7±101.4) for Ae. aegypti. Values were more variable between Ae. aegypti females at different days pi.

Bottom Line: The recent CHIKV outbreaks were caused by a new variant characterized by a mutation in the E1 glycoprotein gene (E1-226V) which has favored a better transmissibility by Ae. albopictus.Ae. albopictus is slightly more efficient than Ae. aegypti to transmit the variant E1-226V of CHIKV.These results will help to design an efficient vector control to limit transmission as soon as the first human cases are diagnosed.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur, Génétique moléculaire des Bunyavirus, Paris, France.

ABSTRACT

Background: Aedes aegypti and Aedes albopictus are potential vectors of chikungunya virus (CHIKV). The recent CHIKV outbreaks were caused by a new variant characterized by a mutation in the E1 glycoprotein gene (E1-226V) which has favored a better transmissibility by Ae. albopictus. As Ae. albopictus tends to replace Ae. aegypti in many regions, one question remained: is Ae. albopictus as efficient as Ae. aegypti to transmit the variant E1-226V of CHIKV?

Methodology and findings: We infected orally both species with the variant E1-226V and estimated the infection, the viral dissemination, and the transmission rate by real time RT-PCR. Additionally, we used an in vitro assay to determine the amount of virus delivered by mosquitoes in their saliva. We found that Ae. aegypti as well as Ae. albopictus ensured a high replication of the virus which underwent an efficient dissemination as detectable in the salivary glands at day 2 post-infection (pi). Infectious CHIKV particles were delivered by salivary glands from day 2 with a maximum at day 6 pi for Ae. albopictus (10(3.3) PFU) and day 7 pi for Ae. aegypti (10(2.5) PFU).

Conclusions: Ae. albopictus is slightly more efficient than Ae. aegypti to transmit the variant E1-226V of CHIKV. These results will help to design an efficient vector control to limit transmission as soon as the first human cases are diagnosed.

Show MeSH
Related in: MedlinePlus