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Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines.

Müller-Richter UD, Dowejko A, Reuther T, Kleinheinz J, Reichert TE, Driemel O - Head Face Med (2009)

Bottom Line: The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference.MAGE-A antigens are expressed in oral squamous cell carcinomas.MAGE-A antigens are still an interesting aim for immunotherapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dpt of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Würzburg, Germany. mueller_u2@klinik.uni-wuerzburg.de

ABSTRACT

Background: The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression profiles also modulate the course of the cancer disease and its response to antineoplastic drugs.

Methods: The expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in five own oral squamous cell carcinoma cell lines were characterised by rt-PCR, qrt-PCR and immunocytochemistry with a global MAGE-A antibody (57B) and compared with those of an adult keratinocyte cell line (NHEK).

Results: All tumour cell lines expressed MAGE-A antigens. The antigens were expressed in groups with different preferences. The predominant antigens expressed were MAGE-A2, -A3 and -A6. MAGE-A10 was not expressed in the cell lines tested. The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference.

Conclusion: MAGE-A antigens are expressed in oral squamous cell carcinomas. The expression profiles measured facilitate distinct examinations in forthcoming studies on responses to antineoplastic drugs or radiation therapy. MAGE-A antigens are still an interesting aim for immunotherapy.

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Slide with NHEK cells stained immunocytochemically for MAGE-A and counterstained with hematoxylin (400× magnification). Only a single cell was found with antibody staining.
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Figure 6: Slide with NHEK cells stained immunocytochemically for MAGE-A and counterstained with hematoxylin (400× magnification). Only a single cell was found with antibody staining.

Mentions: For further analysis, immunocytochemical staining and hematoxylin counterstaining of cultured cells was performed (Figures 6, 7, 8, 9, 10, 11). The staining patterns differed significantly. In the NHEK adult keratinocyte cell line, only a sole cell in the whole slide was stained with the 57B antibody (Figure 6). The results for each cell line are described in detail.


Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines.

Müller-Richter UD, Dowejko A, Reuther T, Kleinheinz J, Reichert TE, Driemel O - Head Face Med (2009)

Slide with NHEK cells stained immunocytochemically for MAGE-A and counterstained with hematoxylin (400× magnification). Only a single cell was found with antibody staining.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2690579&req=5

Figure 6: Slide with NHEK cells stained immunocytochemically for MAGE-A and counterstained with hematoxylin (400× magnification). Only a single cell was found with antibody staining.
Mentions: For further analysis, immunocytochemical staining and hematoxylin counterstaining of cultured cells was performed (Figures 6, 7, 8, 9, 10, 11). The staining patterns differed significantly. In the NHEK adult keratinocyte cell line, only a sole cell in the whole slide was stained with the 57B antibody (Figure 6). The results for each cell line are described in detail.

Bottom Line: The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference.MAGE-A antigens are expressed in oral squamous cell carcinomas.MAGE-A antigens are still an interesting aim for immunotherapy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dpt of Oral and Maxillofacial Plastic Surgery, University Hospital Würzburg, Würzburg, Germany. mueller_u2@klinik.uni-wuerzburg.de

ABSTRACT

Background: The immunological response to solid tumours is insufficient. Therefore, tumour specific antigens have been explored to facilitate the activation of the immune system. The cancer/testis antigen class of MAGE-A antigens is a possible target for vaccination. Their differential expression profiles also modulate the course of the cancer disease and its response to antineoplastic drugs.

Methods: The expression profiles of MAGE-A2, -A3, -A4, -A6 and -A10 in five own oral squamous cell carcinoma cell lines were characterised by rt-PCR, qrt-PCR and immunocytochemistry with a global MAGE-A antibody (57B) and compared with those of an adult keratinocyte cell line (NHEK).

Results: All tumour cell lines expressed MAGE-A antigens. The antigens were expressed in groups with different preferences. The predominant antigens expressed were MAGE-A2, -A3 and -A6. MAGE-A10 was not expressed in the cell lines tested. The MAGE-A gene products detected in the adult keratinocyte cell line NHEK were used as a reference.

Conclusion: MAGE-A antigens are expressed in oral squamous cell carcinomas. The expression profiles measured facilitate distinct examinations in forthcoming studies on responses to antineoplastic drugs or radiation therapy. MAGE-A antigens are still an interesting aim for immunotherapy.

Show MeSH
Related in: MedlinePlus